Effect of protected Methionine and Lysine on Milk yield and Composition in Holstein Dairy Cow under Different Dietary Crude Protein Levels : Meta-analysis

사료 내 수준별 조단백질 조건에서 보호메티오닌과 보호라이신의 사료첨가 급여가 착유우유생산성 및 유성분에 미치는 영향 : 메타분석

  • 최낙진 (전북대학교 동물자원과학과)
  • Received : 2016.11.07
  • Accepted : 2016.11.17
  • Published : 2016.11.30


The present study was conducted to investigate effect of dietary protected amino acid on milk yield and composition in dairy cow using meta-analysis. Total 21 research papers were employed in analysis, and mixed model was used for the analysis of effects. Effect of protected methionine (PM) and combination of protected methionine and lysine (PML) were investigated under two different levels of dietary crude protein (CP, <18% and >18%). For performance of dairy cow, milk yield, milk composition including milk fat and protein content and yield and 4% FCM (fat corrected milk) production were used for analysis. In case of milk yield, a trend of increment was found at PM supplementation at low CP (P=0.055). However, the effect of PM at high CP was detected as not significant (P>0.05). In case of milk protein, inclusion of PM at low CP showed significant decrement (P<0.05). However, there was no significant effect of MP on milk protein at high CP (P>0.05). Supplementation of MP at high CP level showed significant increment of milk fat (P<0.05). MP supplementation represented significant increment of 4% FCM production (P<0.05) regardless of dietary CP levels. Effects of PML on milk yield and composition at both of low and high dietary CP were not significant in this study. However, it seem to be that there was a possible positive effect of MPL application at high dietary CP on performance of dairy cow.


  1. Armentano, L., S. Bertics, and G. Ducharme. 1997. Response of Lactating Cows to Methionine or Methionine Plus Lysine Added to High Protein Diets Based on Alfalfa and Heated Soybeans. J. Dairy Sci. 80: 1194-1199.
  2. Bach, A., G. B. Huntington, S. Calsamiglia, and M. Stern. 2000. Nitrogen Metabolism of Early Lactation Cows Fed Diets with Two Different Levels of Protein and Different Amino Acid Profiles. J. Dairy Sci. 83: 2585-2595.
  3. Baker, M., H. Amos, A. Nelson, C. Williams, and M. Froetschel. 1996. Undegraded intake Protein: Effects on Milk Production and Amino Acid Utilization by Cows Fed Wheat Silage. Canadian J. Anim. Sci. 76: 367-376.
  4. Christensen, R., G. Lynch, J. Clark, and Y. Yu. 1993. influence of Amount and Degradability of Protein on Production of Milk and Milk Components by Lactating Holstein Cows. J. Dairy Sci. 76: 3490-3496.
  5. Clark, J. H. 1975. Lactational Responses to Postruminal Administration of Proteins and Amino Acids. J. Dairy Sci. 58: 1178-1197.
  6. Cressman, S. G., D. Grieve, G. Macleod, E. E. Wheeler, and L. Young. 1980. Influence of Dietary Protein Concentration on Milk Production by Dairy Cattle in Early Lactation. J. Dairy Sci. 63: 1839-1847.
  7. Cunningham, K., M. Cecava, T. Johnson, and P. Ludden. 1996. Influence of Source and Amount of Dietary Protein on Milk Yield by Cows in Early Lactation. J. Dairy Sci. 79: 620-630.
  8. Dinn, N., J. Shelford, and L. Fisher. 1998. Use of the Cornell Net Carbohydrate and Protein System and Rumen-Protected Lysine and Methionine to Reduce Nitrogen Excretion from Lactating Dairy Cows. J. Dairy Sci. 81: 229-237.
  9. Doepel, L., D. Pacheco, J. J. Kennelly, M. D. Hanigan, I. F. Lopez, and H. Lapierre. 2004. Milk Protein Synthesis as a Function of Amino Acid Supply. J. Dairy Sci. 87: 1279-1297.
  10. Erdman, R., and J. Vandersall. 1983. Effect of Rumen Protein Degradability on Milk Yield of Dairy Cows in Early Lactation. J. Dairy Sci. 66: 1873-1880.
  11. Forster, R. J., D. Grieve, J. Buchanan-Smith, and G. Macleod. 1983. Effect of Dietary Protein Degradability on Cows in Early Lactation. J. Dairy Sci. 66: 1653-1662.
  12. Henderson, S. J., H. Amos, and J. Evans. 1985. Influence of Dietary Protein Concentration and Degradability on Milk Production, Composition, and Ruminal Protein Metabolism. J. Dairy Sci. 68: 2227-2237.
  13. Henson, J. E., D. J. Schingoethe, and H. A. Maiga. 1997. Lactational Evaluation of Protein Supplements of Varying Ruminal Degradabilities. J. Dairy Sci. 80: 385-392.
  14. Holter, J., H. Hayes, W. Urban, S. Ramsey, and H. Rideout. 1992. Response of Holstein Cows to Corn Gluten Meal Used to Increase Undegradable Protein in Early or Later Lactation. J. Dairy Sci. 75: 1495-1506.
  15. Hristov, A., R. Etter, J. Ropp, and K. Grandeen. 2004. Effect of Dietary Crude Protein Level and Degradability on Ruminal Fermentation and Nitrogen Utilization in Lactating Dairy Cows. J. Anim. Sci. 82: 3219-3229.
  16. Huber, J. and L. Kung. 1981. Protein and Nonprotein Nitrogen Utilization in Dairy Cattle. J. Dairy Sci. 64: 1170-1195.
  17. Kudrna, V., P. Lang, and P. Mlazovska. 1998. The Effect of Rumen-Protected Methionine on Performance of Dairy Cows. Zivocisna Vyroba-Uzpi (Czech Republic).
  18. Leonardi, C., M. Stevenson, and L. Armentano. 2003. Effect of Two Levels of Crude Protein and Methionine Supplementation on Performance of Dairy Cows. J. Dairy Sci. 86: 4033-4042.
  19. National Research Council. 1971. Nutrient Requirements of Domestic Animals. No. 3. Nutrient Requirements of Dairy Cattle. 4Th Rev. Ed. Nat. Acad. Sci., Washington, DC.
  20. Nichols, J., D. Schingoethe, H. Maiga, M. Brouk, and M. Piepenbrink. 1998. Evaluation of Corn Distillers Grains and Ruminally Protected Lysine and Methionine for Lactating Dairy Cows. J. Dairy Sci. 81: 482-491.
  21. Papas, A., C. Sniffen, and T. V. Muscato. 1984. Effectiveness of Rumen-Protected Methionine for Delivering Methionine Postruminally in Dairy Cows. J. Dairy Sci. 67: 545-552.
  22. Piepenbrink, M., T. Overton, and J. Clark. 1996. Response of Cows Fed a Low Crude Protein Diet to Ruminally Protected Methionine and Lysine. J. Dairy Sci. 79: 1638-1646.
  23. Pisulewski, P., H. Rulquin, J. Peyraud, and R. Verite. 1996. Lactational and Systemic Responses of Dairy Cows to Postruminal Infusions of Increasing Amounts of Methionine. J. Dairy Sci. 79: 1781-1791.
  24. Robinson, P. 1996. Rumen Protected Amino Acids for Dairy Cattle: What Is the Future? Anim. Feed Sci. Tech. 59: 81-86.
  25. Robinson, P., W. Chalupa, C. Sniffen, W. Julien, H. Sato, T. Fujieda, K. Watanabe and H. Suzuki. 1999. Influence of Postruminal Supplementation of Methionine and Lysine, Isoleucine, or All Three Amino Acids on Intake and Chewing Behavior, Ruminal Fermentation, and Milk and Milk Component Production. J. Anim. Sci. 77: 2781-2792.
  26. Rogers, J., U. Krishnamoorthy, and C. Sniffen. 1987. Plasma Amino Acids and Milk Protein Production by Cows Fed Rumen-Protected Methionine and Lysine. J. Dairy Sci. 70: 789-798.
  27. Satter, L. D. and R. E. Roffler. 1975. Nitrogen Utilization in the Dairy Cow. J. Dairy Sci. 58: 1219-1237.
  28. Schwab, C. G., L. Satter, and A. Clay. 1976. Response of Lactating Dairy Cows to Abomasal Infusion of Amino Acids. J. Dairy Sci. 59: 1254-1270.
  29. Schwab, C., M. Socha, and N. Whitehouse. 1993. Opportunities for Rumen Protected Lysine and Methionine in Lactating Dairy Cow Nutrition. In: Rhone-Poulenc Animal Nutrition Symposium. pp. 3-28.
  30. Seymour, W. M., C. E. Polan, and J. H. Herbein. 1990. Effects of Dietary Protein Degradability and Casein or Amino Acid Infusions on Production and Plasma Amino Acids in Dairy Cows. J. Dairy Sci. 73: 735-748.
  31. St-Pierre, N. 2001. Invited Review: Integrating Quantitative Findings from Multiple Studies using Mixed Model Methodology. J. Dairy Sci. 84: 741-755.
  32. Stokes, M., J. Clark, and L. M. Steinmetz. 1981. Performance of Lactating Dairy Cows Fed Methionine or Methionine Analog at Two Concentrations of Dietary Crude Protein. J. Dairy Sci. 64: 1686-1694.
  33. Trinacty, J., L. Krizova, M. Richter, V. Cerny, and J. Riha. 2009. Effect of Rumen-Protected Methionine, Lysine or Both on Milk Production and Plasma Amino Acids of High-Yielding Dairy Cows. Czech J. Anim. Sci. 54: 239-248.
  34. Volden, H., W. Velle, O. M. Harstad, A. Aulie, and O. V. Sjaastad. 1998. Apparent Ruminal Degradation and Rumen Escape of Lysine, Methionine, and Threonine Administered Intraruminally in Mixtures to High-Yielding Cows. J. Anim. Sci. 76: 1232-1240.
  35. Wang, C., H. Liu, Y. Wang, Z. Yang, J. Liu, Y. Wu, T. Yan, and H. Ye. 2010. Effects of Dietary Supplementation of Methionine and Lysine on Milk Production and Nitrogen Utilization in Dairy Cows. J. Dairy Sci. 93: 3661-3670.
  36. Yan, T., J. Frost, R. Agnew, R. Binnie, and C. Mayne. 2006. Relationships among Manure Nitrogen Output and Dietary and Animal Factors in Lactating Dairy Cows. J. Dairy Sci. 89: 3981-3991.