DOI QR코드

DOI QR Code

Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal

Choi, Nu-Ri;Seo, Dong-Won;Choi, Ki-Myung;Ko, Na-Young;Kim, Ji-Ho;Kim, Hyun-Il;Jung, Woo-Young;Lee, Jun-Heon

  • 투고 : 2015.04.17
  • 심사 : 2015.08.12
  • 발행 : 2016.03.01

초록

The porcine major histocompatibility complex (MHC) is called swine leukocyte antigen (SLA), which controls immune responses and transplantation reactions. The SLA is mapped on pig chromosome 7 (SSC7) near the centromere. In this study, 3 class I (SLA-1, SLA-3, and SLA-2) and 3 class II (DRB1, DQB1, and DQA) genes were used for investigation of SLA haplotypes in Yucatan miniature pigs in Korea. This pig breed is a well-known model organism for biomedical research worldwide. The current study indicated that Korean Yucatan pig population had 3 Class I haplotypes (Lr-4.0, Lr-6.0, and Lr-25.0) and 3 class II haplotypes (Lr-0.5, Lr-0.7, and Lr-0.25). The combinations of SLA class I and II haplotype together, 2 homozygous (Lr-4.5/4.5 and Lr-6.7/6.7) and 3 heterozygous (Lr-4.5/6.7, Lr-4.5/25.25, and Lr-6.7/25.25) haplotypes were identified, including previously unidentified new heterozygous haplotypes (Lr-4.5/4.7). In addition, a new SLA allele typing method using Agilent 2100 bioanalyzer was developed that permitted more rapid identification of SLA haplotypes. These results will facilitate the breeding of SLA homozygous Yucatan pigs and will expedite the possible use of these pigs for the biomedical research, especially xenotransplantation research.

키워드

Haplotype;Major Histocompatibility Complex [MHC];Swine Leukocyte Antigen [SLA];Yucatan Miniature Pig

참고문헌

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피인용 문헌

  1. Swine Leukocyte Antigen Diversity in Canadian Specific Pathogen-Free Yorkshire and Landrace Pigs vol.8, pp.1664-3224, 2017, https://doi.org/10.5713/ajas.15.0331

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea