Analysis of Swine Leukocyte Antigen Haplotypes in Yucatan Miniature Pigs Used as Biomedical Model Animal

Choi, Nu-Ri;Seo, Dong-Won;Choi, Ki-Myung;Ko, Na-Young;Kim, Ji-Ho;Kim, Hyun-Il;Jung, Woo-Young;Lee, Jun-Heon

  • Received : 2015.04.17
  • Accepted : 2015.08.12
  • Published : 2016.03.01


The porcine major histocompatibility complex (MHC) is called swine leukocyte antigen (SLA), which controls immune responses and transplantation reactions. The SLA is mapped on pig chromosome 7 (SSC7) near the centromere. In this study, 3 class I (SLA-1, SLA-3, and SLA-2) and 3 class II (DRB1, DQB1, and DQA) genes were used for investigation of SLA haplotypes in Yucatan miniature pigs in Korea. This pig breed is a well-known model organism for biomedical research worldwide. The current study indicated that Korean Yucatan pig population had 3 Class I haplotypes (Lr-4.0, Lr-6.0, and Lr-25.0) and 3 class II haplotypes (Lr-0.5, Lr-0.7, and Lr-0.25). The combinations of SLA class I and II haplotype together, 2 homozygous (Lr-4.5/4.5 and Lr-6.7/6.7) and 3 heterozygous (Lr-4.5/6.7, Lr-4.5/25.25, and Lr-6.7/25.25) haplotypes were identified, including previously unidentified new heterozygous haplotypes (Lr-4.5/4.7). In addition, a new SLA allele typing method using Agilent 2100 bioanalyzer was developed that permitted more rapid identification of SLA haplotypes. These results will facilitate the breeding of SLA homozygous Yucatan pigs and will expedite the possible use of these pigs for the biomedical research, especially xenotransplantation research.


Haplotype;Major Histocompatibility Complex [MHC];Swine Leukocyte Antigen [SLA];Yucatan Miniature Pig


  1. Ando, A., M. Ota, M. Sada, Y. Katsuyama, R. Goto, A. Shigenari, H. Kawata, T. Anzai, T. Iwanaga, and Y. Miyoshi et al. 2005. Rapid assignment of the swine major histocompatibility complex (SLA) class I and II genotypes in Clawn miniature swine using PCR-SSP and PCR-RFLP methods. Xenotransplantation 12:121-126.
  2. Ando, A., N. Imaeda, S. Ohshima, A. Miyamoto, N. Kaneko, M. Takasu, T. Shiina, J. K. Kulski, H. Inoko, and H. Kitagawa. 2014. Characterization of swine leukocyte antigen alleles and haplotypes on a novel miniature pig line, microminipig. Anim. Genet. 45:791-798.
  3. Chardon, P., C. Renard, M. Kirszenbaum, C. Gefotin, D. Cohen, and M. Vaiman. 1985. Molecular genetic analyses of the major histocompatibility complex in pig families and recombinants. Int. J. Immunogenet. 12:139-149.
  4. Fang, M., M. Braunschweig, X. Hu, L. Hu, J. Feng, N. Li, and C. Wu. 2005. Genetic variation of exon 2 of SLA-DQB gene in chinese pigs. Biochem. Genet. 43:119-125.
  5. Gautschi, C. and C. Gaillard. 1990. Influence of major histocompatibility complex on reproduction and production traits in swine. Anim. Genet. 21:161-170.
  6. Ho, C. S., J. K. Lunney, A. Ando, C. Rogel-Gaillard, J. H. Lee, L. B. Schook, and D. M. Smith. 2009a. Nomenclature for factors of the SLA system, update 2008. Tissue Antigens 73:307-315.
  7. Ho, C. S., J. K. Lunney, M. H. Franzo-Romain, G. W. Martens, Y. J. Lee, J. H. Lee, M. Wysocki, R. R. R. Rowland, and D. M. Smith. 2009b. Molecular characterization of swine leucocyte antigen class I genes in outbred pig populations. Anim. Genet. 40:468-478.
  8. Ho, C. S., J. K. Lunney, J. H. Lee, M. H. Franzo-Romain, G. W. Martens, R. R. R. Rowland, and D. M. Smith. 2010. Molecular characterization of swine leucocyte antigen class II genes in outbred pig populations. Anim. Genet. 41:428-432.
  9. Lee, J. H., D. Simond, W. J. Hawthorne, S. N. Walters, A. T. Patel, D. M. Smith, P. J. O'Connell, and C. Moran. 2005. Characterization of the swine major histocompatibility complex alleles at eight loci in westran pigs. Xenotransplantation 12:303-307.
  10. Lunney, J. K. and K. D. Murrell. 1988. Immunogenetic analysis of Trichinella spiralis infections in swine. Vet. Parasitol. 29:179-193.
  11. Mallard, B., B. Wilkie, and B. Kennedy. 1989. Genetic and other effects on antibody and cell mediated immune response in swine leucocyte antigen (SLA)-defined miniature pigs. Anim. Genet. 20:167-178.
  12. Michel, S. G., M. L. L. Madariaga, V. Villani, and K. Shanmugarajah. 2015. Current progress in xenotransplantation and organ bioengineering. Int. J. Surg. 13:239-244.
  13. Naziruddin, B., S. Durriya, D. Phelan, B. F. Duffy, B. Olack, D. Smith, T. Howard, and T. Mohanakumar. 1998. HLA antibodies present in the sera of sensitized patients awaiting renal transplant are also reactive to swine leukocyte antigens 1, 2. Transplantation 66:1074-1080.
  14. Park, K., H. Choi, L. M. Thong, O. J. Kwon, J. H. Kim, H. T. Lee, Y. B. Kim, S. B. Park, and C. Park. 2010. Simple and comprehensive SLA-DQB1 genotyping using genomic PCR and direct sequencing. Tissue antigens 76:301-310.
  15. Renard, C. and M. Vaiman. 1989. Possible relationships between SLA and porcine reproduction. Reprod. Nutr. Dev. 29:569-576.
  16. Sachs, D. H., G. Leight, J. Cone, S. Schwarz, L. Stuart, and S. Rosenberg. 1976. Transplantation in miniature swine: I. Fixation of the major histocompatibility complex. Transplantation 22:559-567.
  17. Shishido, S., B. Naziruddin, T. Howard, and T. Mohanakumar. 1997. Recognition of porcine major histocompatibility complex class I antigens by human CD8+ cytolytic T cell clones. Transplantation 64:340-346.
  18. Smith, D., J. Lunney, G. Martens, A. Ando, J. H. Lee, C. S. Ho, L. Schook, C. Renard, and P. Chardon. 2005a. Nomenclature for factors of the SLA class-I system, 2004. Tissue Antigens 65:136-149.
  19. Smith, D. M., J. K. Lunney, C. S. Ho, G. W. Martens, A. Ando, J. H. Lee, L. Schook, C. Renard, and P. Chardon. 2005b. Nomenclature for factors of the swine leukocyte antigen class II system, 2005. Tissue Antigens 66:623-639.
  20. Thong, L. M., H. Choi, O. J. Kwon, J. H. Kim, Y. B. Kim, J. W. Oh, K. Seo, S. C. Yeom, W. J. Lee, and C. Park. 2011. Systematic analysis of swine leukocyte antigen-DRB1 nucleotide polymorphisms using genomic DNA-based high-resolution genotyping and identification of new alleles. Tissue Antigens 77:572-583.
  21. Tosky, E, T. Engle, E. Davis, G. S. Plastow, S. Premaraj, S. D. kachman, and D. C. Ciobanu. 2015, Swine leukocyte antigen class II Region is associated with variation in Porcine Circovirus 2b susceptibility. In: Proceedings of the Plant & Animal Genome XXIII, San Diego, CA, USA. 234 p.
  22. Xu, X.-C., B. Naziruddin, H. Sasaki, D. M. Smith, and T. Mohanakumar. 1999. Allele-specific and peptide-dependent recognition of swine leukocyte antigen class I by human cytotoxic T-cell clones. Transplantation 68:473-479.
  23. Yamada, K., M. Tasaki, M. Sekijima, R. A. Wilkinson, V. Villani, S. G. Moran, T. A. Cormack, I. M. Hanekamp, J. S. Arn, J. A. Fishman, A. Shimizu, and D. H. Sachs. 2014. Porcine cytomegalovirus infection is associated with early rejection of kidney grafts in a pig to baboon xenotransplantation model. Transplantation 98:411-418.

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Supported by : National Research Foundation of Korea