Delphinidin Suppresses Angiogenesis via the Inhibition of HIF-1α and STAT3 Expressions in PC3M Cells

전립선 암세포에서 delphinidin에 의한 HIF-1α와 STAT3 억제를 통한 혈관내피 성장 인자 발현 저해 효과

  • Kim, Mun-Hyeon (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine) ;
  • Kim, Mi-Hyun (Department of Physical Therapy, Inje University) ;
  • Park, Young-Ja (Department of Clinical Pathology, Sorabol College) ;
  • Chang, Young-Chae (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine) ;
  • Park, Yoon-Yub (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine) ;
  • Song, Hyun-Ouk (Research Institute of Biomedical Engineering, Catholic University of Daegu School of Medicine)
  • 김문현 (대구가톨릭대학교 의용생체공학연구소) ;
  • 김미현 (인제대학교 물리치료학과) ;
  • 박영자 (서라벌대학교 임상병리과) ;
  • 장영채 (대구가톨릭대학교 의용생체공학연구소) ;
  • 박윤엽 (대구가톨릭대학교 의용생체공학연구소) ;
  • 송현욱 (대구가톨릭대학교 의용생체공학연구소)
  • Received : 2015.10.01
  • Accepted : 2015.12.25
  • Published : 2016.02.29


Delphinidin is a blue-red pigment and one of the major anthocyanins in plants. It plays an important role in anti-oxidant, anti-inflammatory, anti-mutagenic and anti-cancer properties. In this study, we investigated the inhibitory effects of delphinidin on vascular endothelial growth factor (VEGF) gene expression, an important factor involved in angiogenesis and tumor progression in human prostate cancer. Delphinidin decreased levels of epidermal growth factor (EGF)-induced VEGF mRNA expression in PC-3M cells. The expression of the EGF-induced hypoxia inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) and signaling transducer and activator of transcription 3 (STAT3) proteins, which are the major transcription factors for VEGF, were inhibited by delphinidin. In addition, delphinidin decreases HRE-promoter reporter gene activity, suggesting that delphinidin can suppress the transcription of HIF-$1{\alpha}$ under EGF induction, leading to a decrease in the expression of VEGF. Delphinidin specifically suppressed the phosphorylation of Akt, p70S6K, and 4EBP1, but not the phosphorylation of EGFR. Therefore, our results suggest that delphinidin may inhibit human prostate cancer progression and angiogenesis by inhibiting HIF-$1{\alpha}$, STAT3 and VEGF gene expression.


Supported by : 대구가톨릭대학교


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