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The Anti-melanogenic Effect of Geranium krameri Extract

선이질풀 추출물의 멜라닌 생성 억제 효과

  • Kim, Hyun-Soo (Department of Food science and Technology, Jungwon University)
  • 김현수 (중원대학교 식품공학과)
  • Received : 2015.11.11
  • Accepted : 2015.12.04
  • Published : 2016.02.29

Abstract

We investigated the application of functional materials by examining a variety of physiological activities of Geranium krameri extract obtained from the National Institute of Horticultural and Herbal Science. Geranium krameri extract had a low cytotoxicity against murine melanoma B16F10 cells. At concentrations with little or no cytotoxicity, Geranium krameri extract showed high DPPH radical scavenging activity ($IC_{50}$, $8.72{\mu}g/mL$) and anti-microbial activities against Bacillus subtilis, Escherichia coli, and Candida albicans. Additionally, Geranium krameri extract inhibited tyrosinase activity ($IC_{50}$, $456.86{\mu}g/mL$) and decreased melanin content ($IC_{50}$, $50.35{\mu}g/mL$). The treatment of B16F10 cells with Geranium krameri extract suppressed the protein expression of tyrosinase in a dose-dependent manner. These findings suggest that Geranium krameri extract inhibits melanin synthesis in murine melanoma B16F10 cells by suppressing intracellular tyrosinase expression, as well as directly inhibits tyrosinase activity simultaneously.

Acknowledgement

Supported by : 한국연구재단(NRF)

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