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Effects of Opuntia ficus-indica var. saboten Ripe Fruits on Protection of Neuronal PC-12 Cells and Cholinesterase Inhibition

백년초의 PC-12 신경세포 보호 및 콜린가수분해효소(cholinesterase) 저해 효과

  • Hwang, Jeong-Seung (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Im, Sungbin (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lee, Inil (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Kim, Tae-Rahk (R&D Center, Cellbion Co., Ltd.) ;
  • Kim, Dae-Ok (Department of Food Science and Biotechnology, Kyung Hee University)
  • 황정승 (경희대학교 식품생명공학과) ;
  • 임성빈 (경희대학교 식품생명공학과) ;
  • 이인일 (경희대학교 식품생명공학과) ;
  • 김태락 ((주)셀비온) ;
  • 김대옥 (경희대학교 식품생명공학과)
  • Received : 2016.01.09
  • Accepted : 2016.02.21
  • Published : 2016.02.29

Abstract

Oxidative stress caused by reactive oxygen species is ascribed to many neurodegenerative diseases like Alzheimer's disease. Phenolic antioxidants can reduce the oxidative stress. In this study, ripe fruits of Opuntia ficus-indica var. saboten (OFS) were extracted using 80% (v/v) aqueous ethanol. Total phenolic and flavonoid contents of the OFS fruits (100 g) were 409.9 mg gallic acid equivalents and 72.2 mg catechin equivalents, respectively. The OFS fruits had antioxidant capacity at 381.2, 298.2, and 3,219.9 mg vitamin C equivalents/100 g in ABTS, DPPH, and ORAC assays, respectively. The OFS fruits showed protective effects on PC-12 cells against oxidative stress in a dose-dependent manner, partly due to decrease of intracellular oxidative stress. Furthermore, the OFS fruits inhibited both acetylcholinesterase and butyrylcholinesterase. Consequently, these results suggest that the OFS fruits might be served as a source of functional materials to reduce oxidative stress in neuronal cells and to inhibit cholinesterases.

Keywords

acetylcholinesterase;Opuntia ficus-indica var. saboten;oxidative stress;total phenolic compound;vitamin C equivalent

Acknowledgement

Supported by : 농림수산식품부

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  1. Phytochemical Contents and Antioxidant Activities of Opuntia ficus-indica var. saboten vol.29, pp.5, 2016, https://doi.org/10.9799/ksfan.2016.29.5.767