Toxic Effects of Alumina Nanoparticles in Rat Cerebrums and Kidneys

산화알루미늄 나노물질이 랫드의 대뇌와 신장에 미치는 영향

Jo, Eunhye;Seo, Gyun-Baek;Kim, Hyunmi;Choi, Kyunghee;Kwon, Jung-Taek;Kim, Philje;Eom, Igchun

  • Received : 2015.10.29
  • Accepted : 2015.12.09
  • Published : 2016.02.29


Objectives: Alumina nanoparticles ($Al_2O_3$, Al-NPs) are used for various purposes, including as coating agents and paint additives. Their potential toxicity has raised concern for human health. This study focuses on exploring the toxic effects on the brain and kidneys caused by Al-NPs exposure in rats. Methods: The animals were orally administered Al-NPs at 10, 50 and 100 mg/kg body weight for 28 days following OECD TG 407. To determine the targeted toxicity of Al-NPs, histopathological examination and gene expression analysis were conducted on the rats. Results: The Al-NPs treatment induced kidney tubular dilatation. In the rat cerebrums, the expression levels of 126 genes experienced two-fold or greater increases in response to Al-NPs, including other genes encoding proteins involved in cell differentiation, transcription and signal transduction. In the rat kidneys, the expression levels of 152 genes also showed two-fold or greater increases in response to Al-NPs, including other genes encoding proteins involved in apoptosis, transcription and signal transduction. Conclusion: These results suggest that exposure to Al-NPs influences cellular signal pathways of kidney and cerebrum, and it can be a toxic indicators of nanometrials.


Aluminum oxide;nanoparticles;toxicogenomics


  1. Krewski D, Yokel RA, Nieboer E, Borchelt D, Cohen J, Harry J, et al. Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide. J Toxicol Environ Health B Crit Rev 2007; 10 Suppl 1:1-269.
  2. Oesterling E, Chopra N, Gavalas V, Arzuaga X, Lim EJ, Sultana R, et al. Alumina nanoparticles induce expression of endothelial cell adhesion molecules. Toxicol Lett 2008; 178(3): 160-166.
  3. Zhang QL, Li MQ, Ji JW, Gao FP, Bai R, Chen CY, et al. In vivo toxicity of nano-alumina on mice neurobehavioral profiles and the potential mechanisms. Int J Immunopathol Pharmacol 2011; 24(1 Suppl): 23S-29S.
  4. Zhang Q, Xu L, Wang J, Sabbioni E, Piao L, Di Gioacchino M, et al. Lysosomes involved in the cellular toxicity of nano-alumina: combined effects of particle size and chemical composition. J Biol Regul Homeost Agents 2013; 27(2): 365-375.
  5. Park EJ, Lee GH, Yoon C, Jeong U, Kim Y, Cho MH, et al. Biodistribution and toxicity of spherical aluminum oxide nanoparticles. J Appl Toxicol 2015; Epub ahead of print.
  6. Kura AU, Saifullah B, Cheah PS, Hussein MZ, Azmi N, Fakurazi S. Acute oral toxicity and biodistribution study of zinc-aluminium-levodopa nanocomposite. Nanoscale Res Lett 2015; 10: 105.
  7. Chen L, Yokel RA, Hennig B, Toborek M. Manufactured aluminum oxide nanoparticles decrease expression of tight junction proteins in brain vasculature. J Neuroimmune Pharmacol 2008; 3(4): 286-295.
  8. Shatkin JA, Abbott LC, Bradley AE, Canady RA, Guidotti T, Kulinowski KM, et al. Nano risk analysis: advancing the science for nanomaterials risk management. Risk Anal 2010; 30(11): 1680-1687.
  9. Rittner MN. Market analysis of nanostructured materials. Am. Ceram. Soc. Bull. 2002; 81: 33-26.
  10. Bar-Ilan O, Albrecht RM, Fako VE, Furgeson DY. Toxicity assessments of multisized gold and silver nanoparticles in zebrafish embryos. Small 2009; 5(16): 1897-910.
  11. Lee YS. Laboratory Annimal Medicine. SNU Press 1997.
  12. Kwon JT, Seo GB, Lee MM, Kim HM, Sim IS, Jo EH, et al. Pulmonary toxicity assessment of aluminum oxide nanoparticles via nasal instillation exposure. Korean Journal of Environmental Health Sciences 2013; 39(1): 48-55.
  13. Field MJ, Stanton BA, Giebisch GH. Differential acute effects of aldosterone, dexamethasone, and hyperkalemia on distal tubular potassium secretion in the rat kidney. J Clin Invest 1984; 74(5): 1792-1802.
  14. National Institute of Environment Research. HPV Chemical Initial Risk Assessment : Potassium monohydregen phosphate. Ministry of Environment 2011.
  15. Kim HJ, Park KS. Excretion, tissue distribution and toxicities of titanium oxide nanoparticles in rats after oral administration over five consecutive days. Korean Journal of Environmental Health Sciences 2014; 40(4): 294-303.