Changes in Quality of Hanwoo Bottom Round under Different Freezing and Thawing Conditions

한우육의 냉동 및 해동 조건에 따른 품질 변화

  • Chun, Ho Hyun (Advanced Process Technology and Fermentation Research Group, World Institute of Kimchi) ;
  • Choi, Eun Ji (Advanced Process Technology and Fermentation Research Group, World Institute of Kimchi) ;
  • Han, Ae Ri (Advanced Process Technology and Fermentation Research Group, World Institute of Kimchi) ;
  • Chung, Young Bae (Advanced Process Technology and Fermentation Research Group, World Institute of Kimchi) ;
  • Kim, Jin Se (Postharvest Engineering Division, National Academy of Agricultural Science, RDA) ;
  • Park, Suk Ho (Postharvest Engineering Division, National Academy of Agricultural Science, RDA)
  • 천호현 (세계김치연구소 신공정발효연구단) ;
  • 최은지 (세계김치연구소 신공정발효연구단) ;
  • 한애리 (세계김치연구소 신공정발효연구단) ;
  • 정영배 (세계김치연구소 신공정발효연구단) ;
  • 김진세 (농촌진흥청 국립농업과학원 수확후관리공학과) ;
  • 박석호 (농촌진흥청 국립농업과학원 수확후관리공학과)
  • Received : 2015.10.16
  • Accepted : 2015.12.23
  • Published : 2016.02.29


This study examined the effects of freezing and thawing conditions on quality of Hanwoo bottom round. The beef samples were frozen by air blast freezing at $-20^{\circ}C$ or ethanol immersion freezing at $-70^{\circ}C$ and then stored at $-20^{\circ}C$ for 10 days. After 10 days of storage, the frozen samples were thawed with air blast thawing at $4^{\circ}C$ or water immersion thawing at $4^{\circ}C$ and subjected to subsequent analyses of drip loss, water holding capacity, thiobarbituric acid reactive substance (TBARS), volatile basic nitrogen (VBN), total aerobic bacteria, and microstructure. Drip loss significantly increased in samples treated with air blast freezing compared to ethanol immersion freezing, whereas freezing and thawing processes had no significant impact on water holding capacity of the samples. Thawing conditions had a much stronger influence on the TBARS and VBN of the samples than freezing conditions. There was no significant difference in the population of total aerobic bacteria among the four samples subjected to one freeze-thaw cycle. In addition, to analyze the effects of freeze-thaw cycle on the quality of beef, three freeze-thaw cycles were performed during storage. Multiple freeze-thaw cycles increased drip loss, TBARS, and VBN and decreased water holding capacity, accelerating microstructural damage. These data indicate that Hanwoo bottom round can be rapidly frozen and thawed by using ethanol immersion freezing and water immersion thawing methods with minimal impact on meat quality.


Supported by : 농촌진흥청


  1. Choi YS, Jeong TJ, Hwang KE, Kim HW, Kim CJ, Sung JM, Oh NS, Kim YB. 2015. Effects of emulsion mapping in different parts of pork and beef. Korean J Food Cook Sci 31: 241-247.
  2. Moon JH, Sung M, Kim JH, Kim BS, Kim Y. 2013. Quality factors of freshness and palatability of Hanwoo from their physicochemical and sensorial properties. Korean J Food Sci An 33: 796-805.
  3. Ministry of Agriculture, Food and Rural Affairs. 2014. Agriculture, food, and rural affairs statistics yearbook. Ministry of Agriculture, Food and Rural Affairs, Sejong, Korea.
  4. Moon YH. 2012. Comparison of quality characteristics among chilled loins obtained from Jeju Black Cattle, Hanwoo and imported Australian beef. J East Asian Soc Dietary Life 22: 497-505.
  5. Kang HJ, Lee HY, Park JD, Kum JS. 2013. Effect of microwave treatment on the physicochemical and microbiological characteristics of beef loin during storage at $4^{\circ}C$. Korean J Food Sci Technol 45: 161-166.
  6. Kim CJ, Lee CH, Lee ES, Ma KJ. 1998. Studies on physicochemical characteristics of frozen beef at as influenced by thawing rates. Korean J Food Sci Ani Resour 18: 142-148.
  7. Park MH, Kwon JE, Kim SR, Won JH, Ji JY, Hwang IK, Kim MR. 2012. Physicochemical and microbiological properties of pork by various thawing methods. J East Asian Soc Dietary Life 22: 298-304.
  8. Kim YB, Woo SM, Jeong JY, Ku SK, Jeong JW, Kum JS, Kim EM. 2013. Temperature changes during freezing and effect of physicochemical properties after thawing on meat by air blast and magnetic resonance quick freezing. Korean J Food Sci An 33: 763-771.
  9. Moon YH. 2012. Effects of dietary mugwort on nutritional composition and physicochemical characteristics of thawed Hanwoo beef. J East Asian Soc Dietary Life 22: 290-297.
  10. Li B, Sun DW. 2002. Novel methods for rapid freezing and thawing of foods-a review. J Food Eng 54: 175-182.
  11. He X, Liu R, Nirasawa S, Zheng D, Liu H. 2013. Effect of high voltage electrostatic field treatment on thawing characteristics and post-thawing quality of frozen pork tenderloin meat. J Food Eng 115: 245-250.
  12. Soyer A, Ozalp B, Dalmis U, Bilgin V. 2010. Effects of freezing temperature and duration of frozen storage on lipid and protein oxidation in chicken meat. Food Chem 120: 1025-1030.
  13. Alizadeh E, Chapleau N, de Lamballerie M, Lebail A. 2007. Effects of freezing and thawing processes on the quality of Atlantic salmon (Salmo salar) fillets. J Food Sci 72: E279-E284.
  14. Ban C, Choi YJ. 2012. Innovative techniques and trends in freezing technology of bakery products. Food Science and Industry 45(4): 9-15.
  15. Yun CG, Lee DH, Park J. 1998. Ohmic thawing of a frozen meat chunk. Korean J Food Sci Technol 30: 842-847.
  16. Xia X, Kong B, Liu J, Diao X, Liu Q. 2012. Influence of different thawing methods on physicochemical changes and protein oxidation of porcine longissimus muscle. LWT-Food Sci Technol 46: 280-286.
  17. Boonsumrej S, Chaiwanichsiri S, Tantratian S, Suzuki T, Takai R. 2007. Effects of freezing and thawing on the quality changes of tiger shrimp (Penaeus monodon) frozen by air-blast and cryogenic freezing. J Food Eng 80: 292-299.
  18. Lee JK, Park J. 1999. Rapid thawing of frozen pork by 915 MHz microwave. Korean J Food Sci Technol 31: 54-61.
  19. Ko SH, Hong GP, Park SH, Choi MJ, Min SG. 2006. Studies on physical properties of pork frozen by various high pressure freezing process. Korean J Food Sci Ani Resour 26: 464-470.
  20. Nam JH, Song HI, Kim MS, Moon YH, Jung IC. 1998. Effects of freezing temperature on quality of thawed beef. Korean J Food & Nutr 11: 482-487.
  21. Jung IC. 1999. Effect of freezing temperature on the quality of beef loin aged after thawing. J Korean Soc Food Sci Nutr 28: 871-875.
  22. AOAC. 1990. Official methods of analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 788.
  23. Rodezno LAE, Sundararajan S, Solval KM, Chotiko A, Li J, Zhang J, Alfaro L, Bankston JD, Sathivel S. 2013. Cryogenic and air blast freezing techniques and their effect on the quality of catfish fillets. LWT-Food Sci Technol 54: 377-382.
  24. Liang D, Lin F, Yang G, Yue X, Zhang Q, Zhang Z, Chen H. 2015. Advantages of immersion freezing for quality preservation of litchi fruit during frozen storage. LWT-Food Sci Technol 60: 948-956.
  25. Ahn DU, Olson DG, Jo C, Chen X, Wu C, Lee JI. 1998. Effect of muscle type, packaging, and irradiation on lipid oxidation, volatile production, and color in raw pork patties. Meat Sci 49: 27-39.
  26. Kim S, Ma Y, Gu K, Lee Y, Kim E, Song KB. 2005. Effect of chlorine dioxide treatment on microbial safety and qaulity of saury during storage. J Korean Soc Food Sci Nutr 34: 1258-1264.
  27. Moon YH. 2013. Changes in physical properties of ham and loin from low-fat pork cuts during chilling after thawing. J East Asian Soc Dietary Life 23: 487-495.
  28. Hwang EG, Bea MJ, Kim BK. 2010. Research on consumers purchasing characteristics and satisfaction for Hanwoo beef. J Korean Soc Food Sci Nutr 39: 709-718.
  29. Jeong GG, Park NY, Lee SH. 2006. Quality characteristics of high and low grade Hanwoo beef during storage at $1^{\circ}C$. Korean J Food Sci Technol 38: 10-15.
  30. Xanthakis E, Le-Bail A, Ramaswamy H. 2014. Development of an innovative microwave assisted food freezing process. Innovative Food Sci Emerging Technol 26: 176-181.
  31. Anese M, Manzocco L, Panozzo A, Beraldo P, Foschia M, Nicoli MC. 2012. Effect of radiofrequency assisted freezing on meat microstructure and quality. Food Res Int 46: 50-54.
  32. Leygonie C, Britz TJ, Hoffman LC. 2012. Impact of freezing and thawing on the quality of meat: review. Meat Sci 91: 93-98.
  33. Eastridge JS, Bowker BC. 2011. Effect of rapid thawing on the meat quality attributes of USDA select beef strip loin steaks. J Food Sci 76: S156-S162.
  34. Kim YH, Liesse C, Kemp R, Balan P. 2015. Evaluation of combined effects of ageing period and freezing rate on quality attributes of beef loins. Meat Sci 110: 40-45.
  35. Kaale LD, Eikevik TM, Rustad T, Nordtvedt TS. 2014. Changes in water holding capacity and drip loss of Atlantic salmon (Salmo salar) muscle during superchilled storage. LWT-Food Sci Technol 55: 528-535.
  36. Hughes JM, Oiseth SK, Purslow PP, Warner RD. 2014. A structural approach to understanding the interactions between colour, water-holding capacity and tenderness. Meat Sci 98: 520-532.
  37. Ali S, Zhang W, Rajput N, Khan MA, Li C, Zhou G. 2015. Effect of multiple freeze-thaw cycles on the quality of chicken breast meat. Food Chem 173: 808-814.
  38. Shin HY, Ku KJ, Park SK, Song KB. 2006. Use of freshness indicator for determination of freshness and quality change of beef and pork during storage. Korean J Food Sci Technol 38: 325-330.
  39. Yamamoto SA, Harris LJ. 2001. The effects of freezing and thawing on the survival of Escherichia coli O157:H7 in apple juice. Int J Food Microbiol 67: 89-96.
  40. Haughton PN, Lyng J, Cronin D, Fanning S, Whyte P. 2012. Effect of crust freezing applied alone and in combination with ultraviolet light on the survival of Campylobacter on raw chicken. Food Microbiol 32: 147-151.
  41. Speck ML, Ray B. 1977. Effects of freezing and storage on microorganisms in frozen foods: A review. J Food Prot 40: 333-336.

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