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Inhibitory Effects of Transglycoslyation Products of Soy Isoflavones on Cholesterol Biosynthesis

대두 이소플라본 당전이 반응 산물의 콜레스테롤 생합성 저해 효과

  • Yoo, Lang Kuk (Department of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Seung Jun (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Moon, Tae Wha (Department of Agricultural Biotechnology, Seoul National University) ;
  • Shim, Jae-Hoon (Department of Food Science and Nutrition, Hallym University)
  • 유랑국 (서울대학교 농생명공학부) ;
  • 최승준 (서울과학기술대학교 식품공학과) ;
  • 문태화 (서울대학교 농생명공학부) ;
  • 심재훈 (한림대학교 식품영양학과)
  • Received : 2015.08.13
  • Accepted : 2015.09.25
  • Published : 2016.02.29

Abstract

Hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) is the rate-limiting enzyme in biosynthesis of cholesterol in animals. In this study, inhibitory effects of isoflavone glycosides on HMG-CoA reductase were investigated. At sample concentration of $100{\mu}M$, genistein-7-O-triglucoside (G2-genistin) inhibited HMG-CoA reductase activity by approximately 18%, whereas daidzein-7-O-triglucoside had no inhibitory effect. In the kinetic experiments with Syrian hamster HMG-CoA reductase, G2-genistin showed inhibitory efficacy with an invariable $V_{max}$ value, suggesting that G2-genistin works as a competitive inhibitor of HMG-CoA reductase and has potential for hypocholesterolemic action through direct regulation of HMG-CoA reductase.

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