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Salt treatment for recovery of the mud loach, Misgurnus mizolepis from transport stress

  • Yu, Jin-Ha (Quarantine and Inspection Division, National Fishery Products Quality Management Service) ;
  • Kim, Dae-Hyun (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Han, Jung-Jo (Division of Fishery Safety, Gyeonggi Province Maritime and Fisheries Research Institute) ;
  • Park, Sung-Woo (Department of Aquatic Life Medicine, Kunsan National University)
  • Received : 2016.05.03
  • Accepted : 2016.08.29
  • Published : 2016.12.31

Abstract

Due to the shortage of the fingerling/juvenile mud loach, Misgurnus mizolepis in Korea, these fish have been imported from China. However, the mortality rate during and after their transportation is very high. In this study, we examined various physiological and histological parameters to evaluate the effect of salt treatment on the survival and recovery of mud loaches in holding farms during the quarantine process. Glucose, osmolality, $Na^+$, $Cl^-$, and histological changes were assessed for three different salinities. Non-treated fish (control 0.0%) exhibited lower levels of osmolality, and $Na^+$ and $Cl^-$ concentrations compared with those kept in solar salt solution (0.5% and 1.0%). Glucose levels in control fish were higher than those in fish exposed to 0.5% and 1.0% solar salt solution. Histologically, control fish showed thinner epidermis of skin, branchial hyperplasia and lamellar fusion with an abundance of eosinophilic granule cell-like cells. After solar salt solution treatment, damaged gill structures in the fish almost recovered within 5 days. The present study demonstrates that mud loaches transported from China suffer from skin and gill damage and physiological dysfunction which may increase the mortality and morbidity. Moreover, saline treatment might alleviate the stress responses and ionic/osmotic imbalances, and help heal gill damage.

Acknowledgement

Supported by : National Fishery Products Quality Management Service (NFQS), Gyeonggi Province Maritime and Fisheries Research Institute, Fisheries Science Institute of Kunsan National University

References

  1. Assem H, Hanke W. Cortisol and osmotic adjustment of the euryhaline teleost, Sarotherodon mossambicus. Gen Comp Endocrinol 1981, 43, 370-380. https://doi.org/10.1016/0016-6480(81)90297-5
  2. Acerete L, Balasch JC, Espinosa E, Josa A, Tort L. Physiological responses in Euraisan perch (Perca fluviatilis, L.) subjected to stress by transport and handling. Aquaculture 2004, 237, 167-178. https://doi.org/10.1016/j.aquaculture.2004.03.018
  3. Ahn SJ, Sung JH, Kim NY, Lee AR, Jeon SJ, Lee JS, Kim JK, Chung JK, Lee HH. Molecular cloning, expression, and characterization of cathepsin L from mud loach (Misgurnus mizolepis). Appl Biochem Biotechnol 2010, 162, 1858-1871. https://doi.org/10.1007/s12010-010-8964-6
  4. Barton BA, Iwama GK. Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Ann Rev Fish Dis 1991, 1, 3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  5. Barton BA, Zitzow RE. Physiological responses of juvenile walleyes to handling stress with recovery in saline water. Prog Fish-Cult 1995, 57, 267-276. https://doi.org/10.1577/1548-8640(1995)057<0267:PROJWT>2.3.CO;2
  6. Bond CE. Biology of Fishes. 2nd ed. pp. 402-403, Brooks Cole, Pacific Grove, 1996.
  7. Baldisserotto B. Osmoregulatory adaptations of freshwater teleosts. In: Val AL, Kapoor BG. (eds.). Fish Adaptations. pp. 179-201, Science Publishers, Enfield, 2003.
  8. Carmichael GJ, Wedemeyer GA, McCraren JP, Millard JL. Physiological effects of handling and hauling stress on smallmouth bass. Prog Fish-Cult 1983, 45, 110-113. https://doi.org/10.1577/1548-8659(1983)45[110:PEOHAH]2.0.CO;2
  9. Carmichael GJ, Tomasso JR, Simco BA, Davis KB. Characterization and alleviation of stress associated with hauling largemouth bass. Trans Am Fish Soc 1984, 113, 778-785. https://doi.org/10.1577/1548-8659(1984)113<778:CAAOSA>2.0.CO;2
  10. Cech JJ Jr, Bartholow SD, Young PS, Hopkins TE. Striped bass exercise and handling stress in freshwater: physiological responses to recovery environment. Trans Am Fish Soc 1996, 125, 308-320. https://doi.org/10.1577/1548-8659(1996)125<0308:SBEAHS>2.3.CO;2
  11. Carneiro PCF, Urbinati EC. Salt as a stress response mitigator of matrinxa, Brycon cephalus (Gunther), during transport. Aquac Res 2001, 32, 297-304. https://doi.org/10.1046/j.1365-2109.2001.00558.x
  12. Chandroo KP, Cooke SJ, McKinley RS, Moccia RC. Use of electromyogram telemetry to assess the behavioural and energetic responses of rainbow trout, Oncorhynchus mykiss (Walbaum) to transportation stress. Aquac Res 2005, 36, 1226-1238. https://doi.org/10.1111/j.1365-2109.2005.01347.x
  13. de la Torre FR, Ferrari I, Salibian A. Biomarkers of a native fish species (Cnesterodon decemmaculatus) application to the water toxicity assessment of peri-urban polluted river of Argentina. Chemosphere 2005, 59, 577-583. https://doi.org/10.1016/j.chemosphere.2004.12.039
  14. Evans DH, Piermarini PM, Choe KP. The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 2005, 85, 97-177. https://doi.org/10.1152/physrev.00050.2003
  15. Gomes LC, Chagas EC, Brinn RP, Roubach R, Coppati CE, Baldisserotto B. Use of salt during transportation of air breathing pirarucu juveniles (Arapaima gigas) in plastic bags. Aquaculture 2006, 256, 521-528. https://doi.org/10.1016/j.aquaculture.2006.02.004
  16. Hoffmayer ER, Parsons GR. The physiological response to capture and handling stress in the Atlantic sharpnose shark, Rhizoprionodon terraenova. Fish Physiol Biochem 2001, 25, 277-285. https://doi.org/10.1023/A:1023210620904
  17. Iversen M, Finstad B, McKinley RS, Eliassen RA, Carlsen KT, Evjen T. Stress responses in Atlantic salmon (Salmo salar L.) smolts during commercial well boat transports, and effects on survival after transfer to sea. Aquaculture 2005, 243, 373-382. https://doi.org/10.1016/j.aquaculture.2004.10.019
  18. Kim DS, Nam YK, Park IS. Survival and karyological analysis of reciprocal diploid and triploid hybrids between mud loach (Misgurnus mizolepis) and cyprinid loach (Misgurnus anguillicaudatus). Aquaculture 1995, 135, 275-265.
  19. Lee YC, Chang YJ, Lee BK. Osomoregulation capability of juvenile grey mullets (Mugil cephalus) with the different salinities. Korean J Fish Aquat Sci 1997, 30, 216-224.
  20. Mazeaud MM, Mazeaud F, Donaldson EM. Primary and secondary effects of stress in fish: some new data with a general review. Trans Am Fish Soc 1977, 106, 201-212. https://doi.org/10.1577/1548-8659(1977)106<201:PASEOS>2.0.CO;2
  21. McDonald DG, Cavdek V, Ellis R. Gill design in freshwater fishes: interrelationships among gas exchange, ion regulation, and acid-base regulation. Physiol Zoo 1991, 64, 103-123. https://doi.org/10.1086/physzool.64.1.30158515
  22. National Fishery Products Quality Management Service. Statistics system for imported aquatic animal quarantine. NFQS, Busan, 2015.
  23. Noga EJ, Udomkusonsri P. Fluorescein: a rapid, sensitive, nonlethal method for detecting skin ulceration in fish. Vet Pathol 2002, 39, 726-731. https://doi.org/10.1354/vp.39-6-726
  24. Nigro M, Falleni A, Del Barga ID, Scarcelli V, Lucchesi P, Regoli R, Frenzilli G. Cellular biomarkers for monitoring estuarine environments: transplanted versus native mussels. Aquat Toxicol 2006, 77, 339-347. https://doi.org/10.1016/j.aquatox.2005.12.013
  25. Nascimento AA, Araújo FG, Gomes ID, Mendes RMM, Sales A. Fish gills alterations as potential biomarkers of environmental quality in a eutrophized tropical river in southeastern Brazil. Anat Histol Embryol 2012, 41, 209-216. https://doi.org/10.1111/j.1439-0264.2011.01125.x
  26. Pickering AD, Pottinger TG. Biochemical effects of stress. In: Hochachka PW, Mommsen TP (eds.). Biochemistry and Molecular Biology of Fishes. Vol. 5. pp. 349-379, Elsevier, Amsterdam, 1995.
  27. Schreck CB. Stress and compensation in teleostean fishes: response to social and physical factors. In: Pickering AD (ed.). Stress and Fish. pp. 295-321, Academic Press, London, 1981.
  28. Souza-Bastos LR, Freire CA. The handling of salt by the neopropical cultured freshwater catfish Rhamdia quelen. Aquaculture 2009, 289, 167-174. https://doi.org/10.1016/j.aquaculture.2009.01.007
  29. Tsuzuki MY, Ogawa K, Strussmann CA, Maita M, Takashima F. Physiological responses during stress and subsequent recovery at different salinities in adult pejerrey Odontesthes bonariensis. Aquaculture 2001, 200, 349-362. https://doi.org/10.1016/S0044-8486(00)00573-1
  30. Wells RMG, Davie PS. Oxygen binding by the blood and hematological effects of capture stress in two big game-fish: mako shark and striped marlin. Comp Biochem Physiol A Comp Physiol 1985, 81, 643-646. https://doi.org/10.1016/0300-9629(85)91041-2
  31. Wurts WA. Using salt to reduce handling stress in channel catfish. World Aquac 1996, 26, 80-81.
  32. Wendelaar Bonga SE. The stress response in fish. Physiol Rev 1997, 77, 591-625. https://doi.org/10.1152/physrev.1997.77.3.591

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