DOI QR코드

DOI QR Code

Analysis of antibiotic residues in milk from healthy dairy cows treated with bovine mastitis ointment using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry

  • Park, Eun-Kee (Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Ryu, Yong-Jae (Food and Drug Analysis Section, Chungcheongbuk-do Institute of Health and Environment) ;
  • Cha, Chun-Nam (Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Yoo, Chang-Yeul (Department of Smart Information Convergence, Gyeongnam Provincial Namhae College) ;
  • Kim, Suk (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2016.07.31
  • Accepted : 2016.11.15
  • Published : 2016.12.31

Abstract

This study was conducted to analyze penicillin G (PEG), streptomycin (STR) and neomycin (NEO) residues in milk of healthy lactating cows. Milk samples were collected from all four quarters of 12 dairy cows 2−7 days after intramammary infusions of an ointment containing PEG, STR and NEO once (n = 4; group I) or twice (n = 4, group II) daily. Ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry was used to determine the antibiotic residues in the samples. The correlation coefficient ($r^2$) of the calibration curves for all antibiotics was > 0.999 and the limits of detection and quantification were $0.002-0.005{\mu}g/mL$ and $0.007-0.02{\mu}g/mL$, respectively. Recovery rates were ranged from 75.5 to 92.3%. In group I, PEG, STR and NEO residues were detected in milk at 2, 3 and 2 days post-treatment, respectively, which were below the maximum residue limit (MRL). In group II, PEG, STR and NEO residues were detected in milk at 2, 3 and 3 days post-treatment, respectively, which were bellow the MRL. These results suggest that a 3-day for milk withdrawal period after the ointment treatment might be sufficient for reduction of the antibiotic residues below the MRL.

Acknowledgement

Supported by : Daehan New Pharm Co., Ltd.

References

  1. Adetunji VO. Effects of processing on antibiotic residues (streptomycin, penicillin-G and tetracycline) in soft cheese and yoghurt processing lines. Pak J Nutr 2011, 10, 792-795. https://doi.org/10.3923/pjn.2011.792.795
  2. Babapour A, Azami L, Fartashmehr J. Overview of antibiotic residues in beef and mutton in Ardebil, North West of Iran. World Appl Sci J 2012, 19, 1417-1422.
  3. Bannerman DD. Pathogen-dependent induction of cytokines and other soluble inflammatory mediators during intramammary infection of dairy cows. J Anim Sci 2009, 87 (13 Suppl), 10-25. https://doi.org/10.2527/jas.2008-1187
  4. Blum SE, Heller ED, Leitner G. Long term effects of Escherichia coli mastitis. Vet J 2014, 201, 72-77. https://doi.org/10.1016/j.tvjl.2014.04.008
  5. Codex Alimentarius Commission. Maximum residue limits (MRLs) and risk management recommendations (RMRs) for residues of veterinary drugs in foods. Updated as at the 38th session of the Codex Alimentarius Commission (July 2015). pp. 6-28, Food and Agriculture Organization in the United Nations, Rome, 2015.
  6. Diez V, Perez JE, Olivera Angel M, Restrepo JG, Villar D. Evaluation of screening tests for antimicrobial residues in milk from individual cows treated with a combination of penicillins G and streptomycin. Rev CES Med Zootec 2013, 8, 52-60.
  7. Douglas D, Banaszewski K, Juskelis R, Al-Taher F, Chen Y, Cappozzo J, McRobbie L, Salter RS. Validation of a rapid lateral flow test for the simultaneous determination of ${\beta}$-lactam drugs and flunixin in raw milk. J Food Prot 2012, 75, 1270-1277. https://doi.org/10.4315/0362-028X.JFP-11-570
  8. European Union. Commission regulation (EU) No 37/2010 of 22 December 2009 on pharmacologically active substances and their cl.ication regarding maximum residue limits in foodstuffs of animal origin. OJEU 2010, 53, L 15/1-77.
  9. European Union. Decision (2002/657/EC) of 12 August 2002 implementing council directive 96/23/EC concerning the performance of analytical methods and inter- pretation of results. OJEU 2002, 45, L 221/8-36.
  10. Fletouris DJ, Psomas JE, Mantist AJ. Determination of some monobasic penicillins in milk by ion-pair liquid chromatography. J Agric Food Chem 1992, 40, 617-621. https://doi.org/10.1021/jf00016a018
  11. Food and Agriculture Organization of the United Nations. Residues of Some Veterinary Drugs in Animal and Foods. pp. 58-59, FAO, Geneva, 1994.
  12. Gomes F, Henriques M. Control of bovine mastitis: old and recent therapeutic approaches. Curr Microbiol 2016, 72, 377-382. https://doi.org/10.1007/s00284-015-0958-8
  13. Gong Q, Ding L, Zhu S, Jiao Y, Cheng J, Fu S, Wang L. Determination of ten aminoglycoside residues in milk and dairy products using high performance liquid chromatographytandem mass spectrometry. Se Pu 2012, 30, 1143-1147.
  14. Hady PJ, Lloyd JW, Kaneene JB. Antibacterial use in lactating dairy cattle. J Am Vet Med Assoc 1993, 203, 210-220.
  15. Hameed KGA, Sender G, Korwin-Kossakowska A. Public health hazard due to mastitis in dairy cows. Anim Sci Pap Rep 2006, 25, 73-85.
  16. Han RW, Zheng N, Yu ZN, Wang J, Xu XM, Qu XY, Li SL, Zhang YD, Wang JQ. Simultaneous determination of 38 veterinary antibiotic residues in raw milk by UPLC-MS/MS. Food Chem 2015, 181, 119-126. https://doi.org/10.1016/j.foodchem.2015.02.041
  17. Jank L, Hoff RB, Tarouco PC, Barreto F, Pizzolato TM. ${\beta}$-lactam antibiotics residues analysis in bovine milk by LCESI-MS/MS: a simple and fast liquid-liquid extraction method. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2012, 29, 497-507. https://doi.org/10.1080/19440049.2011.604044
  18. Kebede G, Zenebe T, Disassa H, Tolosa T. Review on detection of antimicrobial residues in raw bulk milk in dairy farms. Afr J Basic Appl Sci 2014, 6, 87-97.
  19. Krause KM, Serio AW, Kane TR, Connolly LE. Aminoglycosides: an overview. Cold Spring Harb Perspect Med 2016, 6, a027029. https://doi.org/10.1101/cshperspect.a027029
  20. Kukusamude C, Burakham R, Chailapakul O, Srijaranai S. High performance liquid chromatography for the simultaneous analysis of penicillin residues in beef and milk using ionpaired extraction and binary water-acetonitrile mixture. Talanta 2012, 92, 38-44. https://doi.org/10.1016/j.talanta.2012.01.020
  21. Lim CM, Cho BH, Chung GS, Son SW. Determination of aminoglycosides in milk by liquid chromatography with tandem mass spectrometry. J Prevent Vet Med 2012, 36, 121-130.
  22. Liu C, Wang H, Jiang Y, Du Z. Rapid and simultaneous determination of amoxicillin, penicillin G, and their major metabolites in bovine milk by ultra-high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2011, 879, 533-540. https://doi.org/10.1016/j.jchromb.2011.01.016
  23. Mahmoudi R, Asadpour R, Pajohi Alamoti MR, Golchin A, Kiyani R, Mohammad Pour R, Altafy K. Raw cow milk quality: relationship between antibiotic residue and somatic cell count. Int Food Res J 2013, 20, 3347-3350.
  24. Mesgari Abbasi M, Babaei H, Ansarin M, Nourdadgar AO, Nemati M. Simultaneous determination of tetracyclines residues in bovine milk samples by solid phase extraction and HPLC-FL method. Adv Pharm Bull 2011, 1, 34-39.
  25. Ministry of Food and Drug Safety (KR). Appendix 7. Maximum residue limits in veterinary drugs. In: Food Code. pp. 1-2, Ministry of Food and Drug Safety, Cheongju, 2015.
  26. Moretain JP, Boisseau J. Elimination of aminoglycoside antibiotics in milk following intramammary administration. Vet Q 1993, 15, 112-117. https://doi.org/10.1080/01652176.1993.9694386
  27. Vakulenko SB, Mobashery S. Versatility of aminoglycosides and prospects for their future. Clin Microbiol Rev 2003, 16, 430-450. https://doi.org/10.1128/CMR.16.3.430-450.2003
  28. White DG, McDermott PF. Emergence and transfer of antibiotic resistance. J Dairy Sci 2001, 84 (E Suppl), E151-155. https://doi.org/10.3168/jds.S0022-0302(01)70209-3
  29. Whittem T, Hanlon D. Dihydrostreptomycin or streptomycin in combination with penicillin G in dairy cattle therapeutics: a review and re-analysis of published data. Part 1: clinical pharmacology. N Z Vet J 1997, 45, 178-184. https://doi.org/10.1080/00480169.1997.36022
  30. Young MS, van Tran K, Goh E, Shia JC. A rapid SPEbased analytical method for UPLC/MS/MS determination of aminoglycoside antibiotic residues in bovine milk, muscle, and kidney. J AOAC Int 2014, 97, 1737-1741. https://doi.org/10.5740/jaoacint.13-153