Analysis of antibiotic residues in milk from healthy dairy cows treated with bovine mastitis ointment using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry

  • Park, Eun-Kee (Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Ryu, Yong-Jae (Food and Drug Analysis Section, Chungcheongbuk-do Institute of Health and Environment) ;
  • Cha, Chun-Nam (Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Yoo, Chang-Yeul (Department of Smart Information Convergence, Gyeongnam Provincial Namhae College) ;
  • Kim, Suk (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2016.07.31
  • Accepted : 2016.11.15
  • Published : 2016.12.31


This study was conducted to analyze penicillin G (PEG), streptomycin (STR) and neomycin (NEO) residues in milk of healthy lactating cows. Milk samples were collected from all four quarters of 12 dairy cows 2−7 days after intramammary infusions of an ointment containing PEG, STR and NEO once (n = 4; group I) or twice (n = 4, group II) daily. Ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry was used to determine the antibiotic residues in the samples. The correlation coefficient ($r^2$) of the calibration curves for all antibiotics was > 0.999 and the limits of detection and quantification were $0.002-0.005{\mu}g/mL$ and $0.007-0.02{\mu}g/mL$, respectively. Recovery rates were ranged from 75.5 to 92.3%. In group I, PEG, STR and NEO residues were detected in milk at 2, 3 and 2 days post-treatment, respectively, which were below the maximum residue limit (MRL). In group II, PEG, STR and NEO residues were detected in milk at 2, 3 and 3 days post-treatment, respectively, which were bellow the MRL. These results suggest that a 3-day for milk withdrawal period after the ointment treatment might be sufficient for reduction of the antibiotic residues below the MRL.


Supported by : Daehan New Pharm Co., Ltd.


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