DOI QR코드

DOI QR Code

Application of Ecklonia cava Kjellman by-product as a feed additive: enhancing weight gain, immunity and protection from Salmonella infection in chickens

양계 사료첨가제로서 감태 추출 부산물로 인한 체중증가 및 방어효능 평가

  • Park, Soyeon (Department of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Chung Yoh (Department of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Park, Bokyoung (Department of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Kim, Kiju (Department of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University) ;
  • Park, Keuntae (Milae Resources ML Co. Ltd.) ;
  • Han, Jong Kwon (Milae Resources ML Co. Ltd.) ;
  • Hahn, Tae-Wook (Department of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University)
  • 박소연 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 김정요 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 박보경 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 김기주 (강원대학교 수의과대학 및 동물의학종합연구소) ;
  • 박근태 ((주)미래자원 ML) ;
  • 한종권 ((주)미래자원 ML) ;
  • 한태욱 (강원대학교 수의과대학 및 동물의학종합연구소)
  • Received : 2016.04.27
  • Accepted : 2016.10.04
  • Published : 2016.12.31

Abstract

The Ecklonia cava Kjellman by-product (ECBP) as a feed additive was evaluated in improvement of productivity and immune enhancement against Salmonella Gallinarum (SG). Lohmann Brown chickens proved SG-free were randomly divided into 3 groups of 8 chickens each. Chickens were fed with the experimental diet treatment: T0, Non treatment-commercial feed; T1, commercial feed with 0.5% ECBP; T2, commercial feed with 0.1% Lactobacillus plantarum. In this study, we evaluated the effect of T1 and T2 groups on the body weight and protective efficacy against SG in chickens. The results demonstrated that treatment of T1 group as a feed additive affected significantly body weight gaining in chickens. In addition, T1 group showed a significant different colonization of SG when compared to T2 and T0 groups. We also studied that serum IgG and $interferon-{\gamma}$ levels were significantly different compared with other treatment groups. Therefore, we suggest that ECBP can be used as a good candidate of feed additives in chicken industry.

Acknowledgement

Supported by : 중소기업, 강원대학교 동물의학종합연구소

References

  1. Ahn G, Hwang I, Park E, Kim J, Jeon YJ, Lee J, Park JW, Jee Y. Immunomodulatory effects of an enzymatic extract from Ecklonia cava on murine splenocytes. Mar Biotechnol (NY) 2008, 10, 278-289. https://doi.org/10.1007/s10126-007-9062-9
  2. Ahn MJ, Yoon KD, Min SY, Lee JS, Kim JH, Kim TG, Kim SH, Kim NG, Hur H, Kim J. Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol Pharm Bull 2004, 27, 544-547. https://doi.org/10.1248/bpb.27.544
  3. Berchieri A Jr, Murphy CK, Marston K, Barrow PA. Observations on the persistence and vertical transmission of Salmonella enterica serovars Pullorum and Gallinarum in chickens: effect of bacterial and host genetic background. Avian Pathol 2001, 30, 221-231. https://doi.org/10.1080/03079450120054631
  4. Chang C, Kim S. Studies on avian infectious bronchitis: II. Standardization of an indirect enzyme-linked immunosorbent assay (ELISA) for antibody measurement. Korean J Vet Res 1989, 29, 503-515.
  5. Choi Y, Goel A, Hosseindoust A, Lee S, Kim K, Jeon S, Noh H, Kwon IK, Chae B. Effects of dietary supplementation of Ecklonia cava with or without probiotics on the growth performance, nutrient digestibility, immunity and intestinal health in weanling pigs. Ital J Anim Sci 2016, 15, 62-68. https://doi.org/10.1080/1828051X.2015.1128685
  6. Choi Y, Hosseindoust A, Goel A, Lee S, Kwon IK, Chae BJ. Effects of Ecklonia cava as fucoidan-rich algae on growth performance, nutrient digestibility, intestinal morphology and caecal microflora in weanling pigs. Asian-Australas J Anim Sci 2016. Epub ahead of print. doi: 10.5713/ajas.16.0102.
  7. de Jong HK, Parry CM, van der Poll T, Wiersinga WJ. Host–pathogen interaction in invasive salmonellosis. PLoS Pathog 2012, 8, e1002933. https://doi.org/10.1371/journal.ppat.1002933
  8. Gahan DA, Lynch MB, Callan JJ, O'Sullivan JT, O'Doherty JV. Performance of weanling piglets offered low-, medium- or high-lactose diets supplemented with a seaweed extract from Laminaria spp. Animal 2009, 3, 24-31. https://doi.org/10.1017/S1751731108003017
  9. Gusils C, Chaia AP, Gonzalez S, Oliver G. Lactobacilli isolated from chicken intestines: potential use as probiotics. J Food Prot 1999, 62, 252-256. https://doi.org/10.4315/0362-028X-62.3.252
  10. Jung EY, Lee SK. Anti-oxidant activities and regeneration effect in HaCaT cell line by Jeju Island aboriginal Eclonia cava. J Korean Soc Cosmetol 2007, 13, 1071-1077.
  11. Kim HJ, Lee BK, Seok HB. Production of multiple probiotics and the performance of laying hens by proper level of dietary supplementation. Korea J Poult Sci 2011, 38, 173-179. https://doi.org/10.5536/KJPS.2011.38.3.173
  12. Kim KS, Lee SK, Choi YS, Ha CH, Kim WH. Effects of dietary of by products for seaweed (Eucheuma spinosum) ethanol production process on growth performance, carcass characteristics and immune activity of broiler chicken. Korean J Poult Sci 2013, 40, 105-113. https://doi.org/10.5536/KJPS.2013.40.2.105
  13. Kim KS, Lee YJ, Kang MS, Han SU, Oh BK. Comparison of resistance to fowl typhoid among crossbreed chickens artificially infected with Samonella gallinarum. Korean J Poult Sci 2002, 29, 59-75.
  14. Kim SH, Park SY, Yu DJ, Na JC, Choi CH, Park YY, Lee SJ, Ryu KS. Effects of supplemental Lactobacillus spp. on performance and cecum microflora in broiler. Korean J Poult Sci 2000, 27, 37-41.
  15. Ko YH, Yang HY, Kang SY, Kim ES, Jang IS. Effects of a blend of Prunus mume extract as an alternative to antibiotics on growth performance, activity of digestive enzymes and microflora population in broiler chickens. J Anim Sci Technol 2007, 49, 611-620. https://doi.org/10.5187/JAST.2007.49.5.611
  16. Lee J, Shin Y, Kim K. Screening of bifidobacteria for the development of probiotics inhibiting intestinal pathogenic bacteria. Korean J Microbiol Biotechnol 2014, 42, 211-218. https://doi.org/10.4014/kjmb.1406.06001
  17. Lee JH, Kim SY, Lee JY, Ahammed M, Ohh SJ. Effect of dietary live or killed Kimchi lactic acid bacteria on growth performance, nutrient utilization, gut microbiota and meat characteristics in broiler chicken. Korean J Poult Sci 2013, 40, 57-65. https://doi.org/10.5536/KJPS.2013.40.1.057
  18. Lee JS, Lee SH, Jang YM, Lee JD, Lee BH, Jung JY. Macrophage and anticancer activities of feed additives on ${\beta}$-glucan from Schizophyllum commune in breast cancer cells. J Korean Soc Food Sci Nutr 2011, 40, 949-955. https://doi.org/10.3746/jkfn.2011.40.7.949
  19. Liu JN, Yoshida Y, Wang MQ, Okai Y, Yamashita Y. B cell stimulating activity of seaweed extracts. Int J Immunopharmacol 1997, 19, 135-142.
  20. Mabeau S, Fleurence J. Seaweed in food products: biochemical and nutritional aspects. Trends Food Sci Technol 1993, 4, 103-107. https://doi.org/10.1016/0924-2244(93)90091-N
  21. Park BS. Determination of optimal added-levels of inuloprebiotics for promotion of growing performance in broiler chickens. J Life Sci 2011, 21, 684-692. https://doi.org/10.5352/JLS.2011.21.5.684
  22. Park KT, Oh M, Nam J, Ji K, Han JK. Characterization of mutant strain, Lactobacillus paracasei ML-7 isolated from Kimchi, and its effect on the growth of broiler. Korean J Food Sci Technol 2014, 46, 148-152. https://doi.org/10.9721/KJFST.2014.46.2.148
  23. Park SY, Kim SH, Yu DJ, Lee SJ, Ryu KS. Effects of supplemental Lactobacillus on broiler performance. Korean J Poult Sci 2001, 28, 27-40.
  24. Schneid ADS, Rodrigues KL, Chemello D, Tondo EC, Ayub MAZ, Aleixo JAG. Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat. Braz J Microbiol 2006, 37, 350-355. https://doi.org/10.1590/S1517-83822006000300027
  25. Shivaprasad HL. Fowl typhoid and pullorum disease. Rev Sci Tech 2000, 19, 405-424. https://doi.org/10.20506/rst.19.2.1222
  26. Wang L, Liu C, Chen M, Ya T, Huang W, Gao P, Zhang H. A novel Lactobacillus plantarum strain P-8 activates beneficial immune response of broiler chickens. Int Immunopharmacol 2015, 29, 901-907. https://doi.org/10.1016/j.intimp.2015.07.024
  27. Williams JE, Dillard LH, Hall GO. The penetration patterns of Salmonella typhimurium through the outer structures of chicken eggs. Avian Dis 1968, 12, 445-466. https://doi.org/10.2307/1588161

Cited by

  1. Immunomodulation by Bioprocessed Polysaccharides from <i>Lentinus edodes</i> Mycelia Cultures with Rice Bran in the <i>Salmonella</i> Gallinarum-infected Chicken Macrophages vol.33, pp.5, 2018, https://doi.org/10.13103/JFHS.2018.33.5.383
  2. Influence of Hermetia illucens Larvae-Derived Functional Feed Additives on Immune Function of Broilers vol.27, pp.12, 2018, https://doi.org/10.5322/JESI.2018.27.12.1305