Dose Effects of Bee Venom Pharmacopuncture for the Acute Ankle Sprain in Rats

발목염좌 급성기에 봉독 약침의 용량에 따른 진통 및 회복에 미치는 효과

  • Yang, Seung-Bum (Department of Medical Non-commissioned Officer, Wonkwang Health Science) ;
  • Kim, Jae-Hyo (Department of Meridian & Acupoint, College of Korean Medicine, Wonkwang University)
  • 양승범 (원광보건대학교 의무부사관과) ;
  • 김재효 (원광대학교 한의과대학 경혈학교실)
  • Received : 2016.11.18
  • Accepted : 2016.12.12
  • Published : 2016.12.27


Objectives : The aim of this study was to observe the dose-dependent effects of bee venom (BV) pharmacopuncture on the serious ankle sprain in rats. Methods : The grade III ankle sprain was produced by surgically damaging the lateral ligaments complex of Sprague-Dawley rats. BV pharmacopuncture with the different doses($5{\mu}g/kg$, $10{\mu}g/kg$, $50{\mu}g/kg$) were treated on the different acupoints(GB34, GB39 and GB42) of the affected hind limb, respectively. By measuring foot weight bearing force ratio(FWBFR), the pain levels by ankle sprain and the pain recovery for 7 days were observed under BV pharmacopuncture on each acupoint. Results : In the normal and ankle sprained rat, the BV single administration decreased FWBFR in a dose - dependent pattern. The higher the BV dose, the higher the pain resulted in the normal and the sprained ankles. Especially, the dose - dependent effects of BV resulted in the most pronounced decrease in FWBFR in GB34. The recovery of FWBFR was shown at the low dose($5{\mu}g/kg$) BV and the effect was most remarkable in the BV of GB34, but the others showed no recovery effect compared with the control group. Conclusions : BV pharmacopuncture does not exhibit analgesic effects in acute phase of ankle sprain. However, the recovery of ankle sprain was more effective than the natural recovery in the case of low dose of BV repeated over time. Considering this, it is presumed that it would be important to select appropriate clinic guidelines for acute phase of ankle sprain.


Supported by : Wonkwang University


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