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Antioxidant and digestive enzyme inhibitory effects of Eisenia bicyclis extracted by different methods

추출방법에 따른 대황 추출물의 항산화 및 소화효소 저해 효과

  • Kim, Jin-Hak (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Park, La Young (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Lee, Shin-Ho (Department of Food Science and Technology, Catholic University of Daegu)
  • 김진학 (대구가톨릭대학교 식품가공학과) ;
  • 박나영 (대구가톨릭대학교 식품가공학과) ;
  • 이신호 (대구가톨릭대학교 식품가공학과)
  • Received : 2015.10.17
  • Accepted : 2015.12.09
  • Published : 2016.02.28

Abstract

The antioxidant and digestive enzyme inhibitory effects of Eisenia bicyclis extracted by various extraction methods (RE, reflux extraction; SE, ultrasonification extraction; AE, autoclave extraction; LE, low-temperature high-pressure extraction) were investigated. The extraction yield (55.21%) and the laminarin (39.03%), fucoidan (24.75%), total polyphenol (115.68 mg GAE/g) and flavonoid (36.67 mg RHE/g) contents of AE were higher than those in other methods. The DPPH radical (86.60%, 500 mg%), ABTS radical (58.56%, 25 mg%), nitrite (86.38%, 100 mg%) scavenging activities of the Eisenia bicyclis extracted by AE were higher than those of Eisenia bicyclis extracted by other methods. The ABTS radical and nitrite scavenging activities were above 98% in all tested Eisenia bicyclis extracts and these activities were dependent on its concentration. The inhibitory effects of AE against amylase (50 mg%) and ${\alpha}$-glucosidase (5 mg%) were 64.76% and 86.71%, respectively. The AE showed the best inhibitory effect of Eisenia bicyclis extracts (50 mg%) against trypsin (24.37%) and ${\alpha}$-chymotrypsin (49.05%), respectively. These results suggest that Eisenia bicyclis extracted by AE can be used as a bioactive and functional material in the food industry.

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