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초분광 영상기술을 이용한 수박종자 발아여부 비파괴 선별기술 개발

배형진;서영욱;김대용;;박은수;조병관
Bae, Hyungjin;Seo, Young-Wook;Kim, Dae-Yong;Lohumi, Santosh;Park, Eunsoo;Cho, Byoung-Kwan

  • 투고 : 2015.11.26
  • 심사 : 2016.01.20
  • 발행 : 2016.02.28

초록

본 연구에서는 발아여부를 고속 대량으로 측정 가능한 초분광 영상 시스템 기반의 비파괴 선별기술을 개발하고자 하였다. 수박의 건전종자 96립과 퇴화종자 96립을 초분광 단파적외선 시스템을 이용하여 측정하였으며, 종자발아검증은 국제종자검정협회(ISTA)규정에 맞추어 5~14일 동안 $25^{\circ}C$에서 BP(between paper)법을 이용하여 실시하였다. 스펙트럼 데이터는 초분광 영상 시스템을 이용하여 데이터를 획득하였으며, 종자 판별모델 개발에는 PLS-DA(partial least square - discriminant analysis)를 적용하였다. PLS-DA분석법을 이용한 종자의 발아여부 판별 결과는 mean normalization을 이용하여 데이터 전처리를 이용할 경우가 가장 우수했으며, calibration의 경우 94.7%의 분류 정확도를 보였으며, validation의 경우 84.2%의 분류 정확도를 나타내어 평균적으로 90.8%의 정확도를 보였다. 이러한 결과는 종자가 퇴화하면서 야기되는 구성성분물질의 차이로 인한 것으로 사료되며, 두 그룹의 분류에 주요한 영향을 끼친 요인의 파악 및 적용을 통해 종자선별기의 개발이 가능함을 보였다.

키워드

수박종자;종자 발아율;부분최소자승판별분석;초분광 영상시스템

참고문헌

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피인용 문헌

  1. Assessment of seed quality using non-destructive measurement techniques: a review vol.26, pp.04, 2016, https://doi.org/10.1017/S0960258516000234

과제정보

연구 과제 주관 기관 : 농림축산식품부