The difference of metabolic profile between male and female zebrafish

Yoon, Dahye;Choi, Jin;Choi, Hyeonsoo;Kim, Suhkmann

  • 투고 : 2016.01.12
  • 심사 : 2016.02.19
  • 발행 : 2016.03.20


Various experiments using zebrafish have been highlighted recently in the scientific community. Because it is possible to conduct practical experiment from various neurological research to area of genetic study or toxicity experiment. However, gender difference effects are nearly not considered. If the gender differences of zebrafish are considered it is possible to obtain more accurate data. In this study, zebrafish which have different genders were compared each other with NMR-based metabolomics. The extracts of male and female zebrafish were measured by 600 MHz NMR spectrometer. Statistical analysis and target profiling were conducted. As a result, muscle related metabolites were observed in male zebrafish and nerve related metabolites were observed in female zebrafish.


NMR spectroscopy;Metabolomics;Zebrafish;Pattern recognition;Target profiling


  1. P. McGrath, C. Q. Li, Drug Discovery Today 13, 394 (2008)
  2. H. Kawai, N. Arata, and H. Nakayasu, Glia 36, 406 (2001)
  3. E. R. Lusczek, J. A. Paulo, J. R. Saltzman, V. Kadiyala, P. A. Banks, G. Beilman, and D. L. Conwell, Journal of the Pancreas 14, 161 (2013)
  4. D. Jacob and C. Deborde, A. Moing, Analytical and Bioanalytical Chemistry 405, 5049 (2013)
  5. D. Yoon, M. Lee, S. Kim, and S. Kim, Journal of the Korean Magnetic Resonance Society 17, 1 (2013)
  6. S. J. Park, Journal of the Korean Magnetic Resonance Society 18, 47 (2014)
  7. E. G. Bligh, W. J. Dyer, Canadian Journal of Biochemistry and Physiology 37, 911 (1959)
  8. D. Pharmazie, An International Journal of Pharmaceutical Sciences 61, 218 (2006)
  9. M. F. M. van Stijna, M. A. R. Vermeulen, M. P. C. Siroen, L. N. Wong, M. P. van den Tolb, G. C. Ligthart-Melis, A. P. J. Houdijk, and P. A. M. van Leeuwen, Metabolism 61, 1036 (2012)
  10. J. Ghosn, M. Guiguet, C. Jardel, R. Benyaou, V. Zeller, A. Simon, M. A. Valantin, B. Amellal, L. Assoumo, J. Y. Hogrel, D. Costagliola, C. Katlama, and A. Lombes, Antiviral therapy 10, 543 (2005)
  11. S. Carmans, J. J. A. Hendriks, K. Thewissen, J. V. den Eynden, P. Stinissen, J. M. Rigo, and N. Hellings, Journal of Neuroscience Research 88, 2420 (2010)
  12. R. M. Abra and P. J. Quinn, Lipids and lipid metabolism 431, 631 (1976)

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연구 과제 주관 기관 : Ministry of Oceans and Fisheries