DOI QR코드

DOI QR Code

Expression, Purification and Characterization of the BLM binding region of human Fanconi Anemia Group J Protein

Yeom, Kyuho;Park, Chin-Ju

  • 투고 : 2015.12.18
  • 심사 : 2016.02.19
  • 발행 : 2016.03.20

초록

FANCJ is a DNA helicase which contributes genome stability by resolving G-quadruplex DNA from 5' to 3' direction. In addition to main ATPase helicase core, FANCJ has the protein binding region at its C-terminal part. BRCA1 and BLM are the binding partner of FANCJ and these protein-protein interactions contribute genomic stability and the proper response to replication stress. As the first attempt for studying FANCJ-BLM interaction, we prepared BLM binding region of FANCJ and characterized with CD and NMR spectroscopy. FANCJ (881-941) with N-ter 6xHis was purified as the oligomer. Secondary structure prediction based on CD data revealed that FANCJ (881-941) composed with ${\beta}$ sheet, turn and coils.$^1H-^{15}N$ HSQC spectra showed nonhomogeneous peak intensities with less number of peaks comparing than the number of amino acids in the construct. It indicated that optimization should be necessary for detailed further structural studies.

키워드

Helicase;Protein-Protein interaction;CD;NMR

참고문헌

  1. W. Wang. Nat. Rev. Genet. 8, 735 (2007) https://doi.org/10.1038/nrg2159
  2. P. Castillo Bosch, et al. EMBO J. 33, 2521 (2014) https://doi.org/10.15252/embj.201488663
  3. H. J. Lipps and D. Rhodes. Trends Cell Biol. 19, 414 (2009) https://doi.org/10.1016/j.tcb.2009.05.002
  4. Y. Wu, S. Y. Kazuo, and Jr. R. M. Brosh. Mol. Cell. Biol. 28, 4116 (2008) https://doi.org/10.1128/MCB.02210-07
  5. R. M. Brosh and S. B. Cantor. Front. Genet. 5, 1 (2014)
  6. A. N. Suhasini, et al. Embo J. 30, 692 (2011) https://doi.org/10.1038/emboj.2010.362
  7. R. M. Brosh. Nat. Rev. Cancer. 13, 542 (2013) https://doi.org/10.1038/nrc3560
  8. A. Kamath-Loeb, L. A. Loeb, and M. Fry. PLoS One. 7, (2012)
  9. R. J. Monnat. Semin. Cancer Biol. 20, 329 (2010) https://doi.org/10.1016/j.semcancer.2010.10.002
  10. H.-H. Kim, H.-K. Song, B.-J. Lee, and S. J. Park. J. Korean Magn. Reson. Soc. 19, 88 (2015) https://doi.org/10.6564/JKMRS.2015.19.2.088
  11. M.-Y. Kwon, Y.-J. Seo, Y.-M. Lee, A.-R. And Lee, J.-H. Lee. J. Korean Magn. Reson. Soc. 19, 95 (2015) https://doi.org/10.6564/JKMRS.2015.19.2.095
  12. A. Micsonai, et al. Proc. Natl. Acad. Sci. U. S. A. 112, E3095 (2015) https://doi.org/10.1073/pnas.1500851112
  13. J. S. Richardson and D. C. Richardson. Proc. Natl. Acad. Sci. U. S. A. 99, 2754 (2002) https://doi.org/10.1073/pnas.052706099

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea