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Evaluation of Bioremediation Efficiency of Crude Oil Degrading Microorganisms Depending on Temperature

온도에 따른 원유분해미생물의 생물학적 정화효율 평가

  • Received : 2015.11.27
  • Accepted : 2015.12.23
  • Published : 2016.02.28

Abstract

Bioremediation is one of the most effective ways to remediate TPH-contaminated sites. However, under actual field conditions that are not at the optimum temperature, degradation of microorganisms is generally reduced, which is why the efficiency of biodegradation is known to be significantly affected by the soil temperature. Therefore, in this study, the labscale experiment was conducted using indigenous crude oil degrading microorganisms isolated from crude oil contaminated site to evaluate the remediation efficiency. Crude oil degrading microorganisms were isolated from crude oil contaminated soil and temperature, which is a significant factor affecting the remediation efficiency of land farming, was adjusted to evaluate the microbial crude oil degrading ability, degradation time, and remediation efficiency. In order to assess the field applicability, the remediation efficiency was evaluated using crude oil contaminated soil (average TPH concentration of 10,000 mg/kg or more) from the OO premises. Followed by the application of microorganisms at 30℃, the bioremediation process reduced its initial TPH concentration of 10,812 mg/kg down to 1,890 mg/kg in 56 days, which was about an 83% remediation efficiency. By analyzing the correlation among the total number of cells, the number of effective cells, and TPH concentration, it was found that the number of effective microorganisms drastically increased during the period from 10 to 20 days while there was a sharp decrease in TPH concentration. Therefore, we confirmed the applicability of land farming with isolated microorganisms consortium to crude oil contaminated site, which is also expected to be applicable to bioremediation of other recalcitrant materials.

Keywords

Crude oil contamination;TPH;Bioremediation;Land farming;Crude oil degrading microorganisms

References

  1. Choi, H.J., Oh, B.Y., Han, Y.S., Hur, M.J., and Kim, J.G., 2014, Characterization study of crude oil degrading microbiology isolated from Incheon bay, J. Life Science, 24(6), 694-699. https://doi.org/10.5352/JLS.2014.24.6.694
  2. Chun, M.H., Son, H.J., and Kim, C., 2007, A study on the isolation of the oil-degradation microbes and treatment efficiency in the oil contaminated soil with peat moss, Kor. J. Env. Hlth., 33(5), 462-469.
  3. Environmental protection agency (EPA), 1994, Chapter V. Landfarming - How to evaluate alternative cleanup technologies for underground storage tank sites: A guide for corrective action plan reviewers, 10-11.
  4. Farahat, Laila A. and El-Gendy, Nour Sh., 2008, Biodegradation of baleym mix crude oil in soil microcosm by some locally isolated Egyptian bacterial strains, Soil Sediment Contam., 17, 150-162. https://doi.org/10.1080/15320380701872886
  5. Gao, Y.C., Guo, S.H., Wang, J.N., Li, D., Wang, H., and Zeng, D.H., 2014, Effects of different remediation treatments on crude oil contaminated saline soil, Chemosphere, 117, 486-493. https://doi.org/10.1016/j.chemosphere.2014.08.070
  6. Goodfellow, M., Manfio, G.P., and Chun, J., 1997, Towards a practical species concept for cultivable bacteria, In The Units of Biodiversity Species in Practice, 1997, Chapman and Hall, London, 25-59 p.
  7. Ma, J.J., Ko, H.S., Hwang, J.S., Jung, M.J., Choi, S.I., and Kim, K.J., 1999, The pilot-scale treatability studies of co-composting for the remediation of diesel contaminated soil during the winter, J. Soil Groundw. Environ., 4(2), 193-201.
  8. Kavitha, V., Mandal, A.B., and Gnanamani, A., 2014, Microbial biosurfactant mediated removal and/or solubilization of crude oil contamination from soil and aqueous phase: An approach with Bacillus licheniformis MTCC 5514, Int. Biodeter. Biodegr., 94, 24-30. https://doi.org/10.1016/j.ibiod.2014.04.028
  9. Kim, G.J., Oh, S.T., Lee, C.H., Seo, S.K., Kang, C.H., and Chang, Y.Y., 2008, Enhancement of biodegradation rate of petroleum hydrocarbons-contaminated soil with addition of organic composite nutrients and a chemical oxidation, J. Soil Groundw. Env., 13(3), 59-66.
  10. Lee, S.H., Kim, E.Y., and Choi, H.J., 2007, Effects of organic amendments on heavy mineral oil biodegradation, J. Soil Groundw. Environ., 12(5), 54-63.
  11. Roy, A.S., Baruah, R., Borah, M., Singh, A.K., Boruah, H.P.D., Saikia, N., Deka, M., Dutta, N., and Bora, T.C., 2014, Bioremediation potential of native hydrocarbon degrading bacterial strains in crude oil contaminated soil under microcosm study, Int. Biodeter. Biodegr., 94, 79-89. https://doi.org/10.1016/j.ibiod.2014.03.024
  12. Simons, K.L., Sheppard, P.J., Adetutu, E.M., Kadali, K., Juhasz, A.L., Manefield, M., Sarma, P.M., Lal, B., and Ball, A.S., 2013, Carrier mounted bacterial consortium facilitates oil remediation in the marine environment, Biresource Technol., 134, 107-116. https://doi.org/10.1016/j.biortech.2013.01.152
  13. Stackebrandt, E. and Goebel, B.M., 1994, A place for DNADNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology, Int. J. Syst. Bacteriol., 44, 846-849. https://doi.org/10.1099/00207713-44-4-846
  14. Yu, J.B., Kim, J.H., Kim, G.J., Oh, S.T., Lee, C.H., Park, I.K., and Chang, Y.Y., 2009, A Filed study on the enhancement of landfarming performance using oil-degradable microbes adapted to various temperature range, J. Soil Groundw. Env. 14(5), 10-17.
  15. Zhao Dongfeng, Liu Chunshuang, Liu Lihong, Zhang Yunbo, Liu Qiyou, and Wu Wei-Min, 2011, Selection of functional consortium for crude oil-contaminated soil remediation, Int. Biodeter. Biodegra., 65, 1244-1248. https://doi.org/10.1016/j.ibiod.2011.07.008