고구마 소주 주박에 의한 RAW 264.7 세포주에서 lipopolysaccharide로 유도된 염증 반응의 억제 효과

DOI QR코드

DOI QR Code

이승훈;권민정;김순영;손호용;신우창;김종식
Lee, Seung-Hoon;Kwon, Min-Jeong;Kim, Soon Young;Sohn, Ho-Yong;Shin, Woo-Chang;Kim, Jong-Sik

  • 투고 : 2015.09.11
  • 심사 : 2015.11.07
  • 발행 : 2016.01.30

초록

본 연구에서는 고구마 소주로부터 획득한 에탄올 추출물 및 순차적 유기용매 분획물을 제조하고, 이들 시료가 LPS로 활성화된 마우스 대식세포 RAW 264.7 세포주에서 nitric oxide (NO) 생산과 세포생존율에 미치는 영향을 연구하였다. 처리한 시료 중 ethyl acetate 분획물(KSD-E8-3) 처리군에서 LPS로 염증 유도된 RAW 264.7 세포주의 생존율에는 영향 없이 NO 생성을 현저하게 감소 시킴을 확인하였다. 또한 분획물 KSD-E8-3 처리에 의해서 염증매개 인자인 COX-2, iNOS 그리고 TNF-alpha의 유전자의 발현이 감소되었으며, iNOS 단백질의 발현이 현저히 감소됨을 Western blot으로 확인하였다. 또한, 분획물 KSD-E8-3 처리에 의해 세포내 대표적인 신호 전달 단백질인 3가지의 MAPK 단백질(p38, JNK, ERK1/2)의 인산화가 모두 억제됨을 확인하였다. 종합적으로, 이러한 연구결과는 고구마 소주 주박에 함유된 생리활성 물질에 의한 항염증 활성 및 작용 기전을 이해하는데 도움을 줄 것으로 기대된다.

키워드

Anti-inflammation;lees extracts;organic solvent fractions;RAW 264.7 cell;sweet potato soju

참고문헌

  1. Ju, A., Cho, Y. C. and Cho, S. 2015. Methanol extracts of Xanthium sibiricum roots inhibit inflammatory response via the inhibition of nuclear factor-κB (NF-κB) and signal transducer and activator of transctiption 3 (STAT3) in murine macrophage. J. Ethnopharmacol. DOI: 10.1016/j.jep.2015.07.038. https://doi.org/10.1016/j.jep.2015.07.038
  2. Akira, S. and Takeda, K. 2004. Toll-like receptor signaling. Nat. Rev. Immunol. 4, 499-511. https://doi.org/10.1038/nri1391
  3. Eisinger, A. L., Prescott, S. M., Jones, D. A. and Stafforini, D. M. 2007. The role of cyclooxygenase-2 and prostaglandins in colon cancer. Prostaglandins Other Lipid Mediat. 82, 147-154. https://doi.org/10.1016/j.prostaglandins.2006.05.026
  4. Jacobsen, M. C., Dusart, P. J., Korowicz, K., Bajaj-eliott, M., Hart, S. L., Klein, N. J. and Dixon, G. L. 2015. A critical role for ATF2 transcription factor in the regulation of E-selectin expression in response to non-endotoxin components of Neisseria meningitides. Cell. Microbiol. DOI: 10.1111/cmi.12483. https://doi.org/10.1111/cmi.12483
  5. Jin, J. H., Kim, J. S., Kang, S. S., Son, K. H., Chang, H. W. and Kim, H. P. 2010. Anti-inflammatory and anti-arthritic activity of the roots of sophora flavescens. J. Ethnopharmacol. 127, 589-595. https://doi.org/10.1016/j.jep.2009.12.020
  6. Kang, H. T., Lee, S. H., Kim, S. Y., Kim, M. S., Shin, W. C., Sohn, H. Y. and Kim, J. S. 2014. Anti-proliferative activities of solvent fractions of lees extracts in human colorectal HCT 116 ells. J. Life Sci. 24, 967-972. https://doi.org/10.5352/JLS.2014.24.9.967
  7. Kim, H. G., Shi, C., Bode, A. M. and Dong, Z. 2015. p38α MAPK is required for arsenic-induced cell transformation. Mol. Carcinog. DOI: 10.1002/mc.22331. https://doi.org/10.1002/mc.22331
  8. Li, G., Wulan, H., Song, Z., Paik, P. A., Tsao, M. L., Goodman, G. M., MacEachern, P. T., Downey, R. S., Jankowska, A. J., Rabinowitz, Y. M., Learch, T. B., Song, D. Z., Yuan, J. J., Zheng, S. and Zheng, Z. 2015. Regulatory B cell function is suprresed by smoking and obesity in H. pylori-infected subjects and is correlated with elevated risk of gastric cancer. Plos One 10, e0134591. https://doi.org/10.1371/journal.pone.0134591
  9. Kim, T. Y., Jeon, T. W., Yeo, S. H., Kim, S. B., Kim, J. S. and Kwak, J. S. 2010. Antimicrobial, antioxidant and SOD-like activity effect of Jubak extracts. Kor. J. Food Nutr. 23, 299-305.
  10. Kojda, G. and Harrison, D. 1999. Interactions between NO and reactive oxygen speices : pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc. Res. 43, 562-571. https://doi.org/10.1016/S0008-6363(99)00169-8
  11. Lee, E. S., Ju, H. K., Moon, T. C., Lee, E., Jahng, Y., Lee, S. H. and Chang, H. W. 2004. Inhibition of nitric oxide and tumor necrosis factor-alpha (TNF-alpha) production by prepenone compound through blockade of nuclear factor (NF)-κB activation in cultured murine macrophages. Biol. Pharm. Bull. 27, 617-620. https://doi.org/10.1248/bpb.27.617
  12. Lo, J. Y., Kamarudin, M. N., Hamdi, O. A., Awang, K. and Kadir, H. A. 2015. Curcumenol isolated from Curuma zedoaria suppresses Akt-mediated NF-κB activation and p38 MAPK signaling pathway in LPS-stimulated BV-2 microlial cells. Food Funct. 6, 3550-3559. https://doi.org/10.1039/C5FO00607D
  13. Anderson, N. and Borlak, J. 2008. Molecular mechanisms and therapeutic targets in steatosis and steatoheptitis. Pharmacol. Rev. 60, 311-357. https://doi.org/10.1124/pr.108.00001
  14. Chelsky, Z. L., Yue, P., Kondratyuk, T. P., Paladino, D., Pezzuto, J. M., Cushman, M. and Turkson, J. 2015. A resveratrol analogue promotes ERK MAPK-dependent STAT3 serine and tyrosine phosphorylation alterations and antitumor effects in vitro against human tumor cells. Mol. Pharmacol. 88, 524-533. https://doi.org/10.1124/mol.115.099093
  15. Sahu, B. D., Kumar, J. M. and Sistla, R. 2015. Baicalein, a bioflavonoid, prevents cisplatin-induced acute kidney injury by up-regulating antioxidant defense and down-regulating the MAPKs and NF-κB pathways. Plos One 10, e0134139. https://doi.org/10.1371/journal.pone.0134139
  16. Syed Hussein, S. S., Kamarudin, M. N. and Kadir, H. A. 2015. (+)-Catechin attenuates NF-κB activation through regulation of Akt, MAPK, and AMPK signaling pathways in LPS-induced BV-2 microglial cells. Am. J. Chin. Med. 43, 927-952. https://doi.org/10.1142/S0192415X15500548
  17. Vlkova, M., Ticha, O., Nechvatalova, J., Kalina, T., Litzman, J., Mauri, C. and Blair, P. A. 2015. Regulatory B cells in CVID patients fail to suppress multifunctional IFN-gamma+TNF-alpha+CD4+ T cells differentiation. Clin. Immunol. 29, 292-300.
  18. Xu, C., Chen, G., Yang, W., Xu, Y., Xu, Y., Huang, X., Liu, J., Feng, Y., Xu, Y. and Liu, B. 2015. Hyaluronan ameliorates LPS-induced acute lung injury in mice via Toll-like receptor (TLR) 4-dependent signaling pathway. Int. Immunopharmacol. 28, 1050-1058. https://doi.org/10.1016/j.intimp.2015.08.021
  19. Kim, M. S., Lee, Y, S., Kim, J. S., Shin, W. C. and Sohn, H. Y. 2014. Evaluation of in-vitro antithrombosis activity of lees of Korean traditional wine. J. Life Sci. 24, 865-872. https://doi.org/10.5352/JLS.2014.24.8.865
  20. Kindt, T. J., Goldsby, R. A. and Osborne, B. A. 2007. Innate immunity : Tenneys. Kuby Immunology. New York. Freeman Press 6, 52-73.
  21. Monick, M. M., Arter, A. B., Robeff, P. K., Flaherty, D. M., Peterson, M. W. and Hunninghake, G. W. 2001. Lipopolysaccharide activates Akt in human alveolar macrophages resulting in nuclear accumulation and transcriptional activity of beta-catenin. J. Immunol. 166, 4713-4720. https://doi.org/10.4049/jimmunol.166.7.4713
  22. Park, M. J., Kang, H. T., Kim, M. S., Shin, W. C., Sohn, H. Y. and Kim, J. S. 2014. Anti-inflammatory effects of extracts and their solvent fractions of rice wine lees. J. Life Sci. 24, 843-850. https://doi.org/10.5352/JLS.2014.24.8.843

과제정보

연구 과제번호 : 주박유래 유효성분의 분리 및 동정

연구 과제 주관 기관 : 안동대학교 산학협력단