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Enhancing Enzymatic Digestibility of Miscanthus sinensis using Steam Explosion Coupled with Chemicals

  • Jung, Ji Young ;
  • Yang, Jae-Kyung
  • Received : 2015.10.14
  • Accepted : 2015.12.25
  • Published : 2016.03.25

Abstract

The effect of steam explosion coupled with alkali (1% sodium hydroxide, 1% potassium hydroxide and 15% sodium carbonate) or organosolv solvent (85% methanol, 70% ethanol and dioxane) on the production of sugar, changes in the chemical composition of M. sinensis were evaluated. The steam explosion coupled with 1% potassium hydroxide and dioxane were better as compared with other treatments based on the removals of acid insoluble lignin, and about 89.0% and 85.4%. Enzymatic hydrolysis of steam explosion with 1% potassium hydroxide and dioxane treated M. sinensis, gave a 98.0% and 96.5% of glucose conversion, respectively. These results suggested that pretreatment of M. sinensis with either potassium hydroxide or dioxane could be a promising pretreatment method for glucose production.

Keywords

Miscanthus sinensis;steam explosion;pretreatment;enzymatic hydrolysis;potassium hydroxide;dioxane

References

  1. Ballesteros, M., Negro, Oliva, J.M., Cabanas, A., Manzanares, P., Ballesteros, M. 2006. Ethanol production from steam explosion pretreated wheat straw. Applied Biochemical Biotechnology 130: 278-288.
  2. Cara, C., Ruiz, E., Ballesteros, I., Negro, M.J., Castro, E. 2006. Enhanced enzymatic hydrolysis of olive tree wood by steam exolosion and alkaline peroxide delignification. Process Biochemistry 41: 423-429. https://doi.org/10.1016/j.procbio.2005.07.007
  3. Chandra, R., Takeuchi, H., Hasegawa, T., Kumar, R. 2012. Improving biodegradability and biogas production of wheat straw substrates using sodium hydroxide and hydrothermal pretreatments. Energy 43: 273-282. https://doi.org/10.1016/j.energy.2012.04.029
  4. Chen, W.H., Pen, B.L., Yu, C.T., Hwang, W.S. 2011. Pretreatment efficiency and structural characterization of rice straw by an integrated process of dilute-acid and steam explosion for bioethanol production. Bioresource Technology 102: 2916-2924. https://doi.org/10.1016/j.biortech.2010.11.052
  5. de Vrije, T., de Haas, G.G., Tan, G.B., Keijsers, E.R.P., Claassen, P.A.M. 2002. Pretreatment of Miscanthus for hydrogen production by Thermotoga elfii. International Journal of Hydrogen Energy 27: 1381-1390. https://doi.org/10.1016/S0360-3199(02)00124-6
  6. Fernandez-Bolanos, J., Felizon, B., Heredia, A., Rodriguez, R., Guillen, R., Jimenez, A. 2001. Steam-explosion of olive stones: hemicellulose solubilization and enhancement of enzymatic hydrolysis of cellulose. Bioresource Technology 79: 53-61. https://doi.org/10.1016/S0960-8524(01)00015-3
  7. Guo, G., Li, S., Wang, L., Ren, S., Fang, G. 2013. Separation and characterization of lignin from bio-ethanol production residue. Bioresource Technology 135: 738-741. https://doi.org/10.1016/j.biortech.2012.10.041
  8. Hongzhang, C., Liying, L. 2007. Unpolluted fractionation of wheat straw by steam explosion and ethanol extraction. Bioresource Technology 98(3): 666-676. https://doi.org/10.1016/j.biortech.2006.02.029
  9. Huang, Y., Wei, X., Zhou, S., Liua, M., Tu, Y., Li, A., Chen, P., Wang, Y., Zhang, X., Tai, H. Peng, L., Xia, T. 2015. Steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in G. barbadense and G. hirsutum. Bioresource Technology 181: 224-230. https://doi.org/10.1016/j.biortech.2015.01.020
  10. Kabel, M.A., van den Borne H., Vincken, J.P., Voragen, A.G.J., Schols, H.A. 2007. Structural differences of xylans affect their interaction with cellulose. Carbohydrate Polymer 69: 94-105. https://doi.org/10.1016/j.carbpol.2006.09.006
  11. Kim, Y., Ximenesa, E., Mosiera, N.S., Ladisch, M.R. 2011. Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass. Enzyme and Microbial Technology 48: 408-415. https://doi.org/10.1016/j.enzmictec.2011.01.007
  12. Kumar, R., Mago, G., Balan, V., Wyman, C.E. 2009. Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies. Bioresource Technology 100: 3948-3962. https://doi.org/10.1016/j.biortech.2009.01.075
  13. Li, C., Knierim, B., Manisseri, C., Arora, R., Scheller, H.V., Auer, M., Vogel, K.P., Simmons, B.A., Singh, S. 2010a. Comparison of dilute acid and ionic liquid pretreatment of switchgrass: biomass recalcitrance, delignification and enzymatic saccharification. Bioresource Technology 101: 4900-4906. https://doi.org/10.1016/j.biortech.2009.10.066
  14. Li, X., Tabil, L.G. Panigrahi, S. 2007. Chemical Treatments of Natural Fiber for Use in Natural Fiber-Reinforced Composites: A Review. Journal of Polymers and the Environment 15: 25-33. https://doi.org/10.1007/s10924-006-0042-3
  15. Li, X., Kim, T.H., Nghiem. N.P., 2010b. Bioethanol production from corn stover using aqueous ammonia pretreatment and two-phase simultaneous saccharification and fermentation (TPSSF). Bioresource Technology 101: 5910-5916. https://doi.org/10.1016/j.biortech.2010.03.015
  16. Li, B.Z., Balan, V., Yuan, Y.J., Dale, B.E. 2010c. Process optimization to convert forage and sweet sorghum bagasse to ethanol basedon ammonia fiber expansion (AFEX) pretreatment. Bioresource Technology 101: 1285-1292. https://doi.org/10.1016/j.biortech.2009.09.044
  17. Liu, C., Wyman, C.E. 2003. The effect of flow rate of compressed hot water on xylan, lignin and total mass removal from corn stover. Industrial & Engineering Chemistry Research 42: 5409-5416. https://doi.org/10.1021/ie030458k
  18. Martin, C., Galbe, M., Nilvebrant, N., Jonsson, L.J. 2002. Comparison of the fermentability of enzymatic hydrolyzates of sugarcane bagasse pretreated by steam explosion using different impregnating agents. Applied Biochemistry and Biotechnology 98(1): 699-716.
  19. Montane, D., Farriol, X., Salvado, J., Jollez, P., Chornet. E. 1998. Fractionation of Wheat Straw by Steam-Explosion Pretreatment and Alkali Delignification. Cellulose Pulp and Byproducts from Hemicellulose and Lignin. Journal of Wood Chemistry and Technology 18(2): 171-191. https://doi.org/10.1080/02773819809349575
  20. Pang, Y.Z., Liu, Y.P., Li, X.J., Wang, K.S., Yuan, H.R. 2008. Improving biodegradability and biogas production of corn stover through sodium hydroxide solid state pretreatment. Energy and Fuels 22: 2761-2766. https://doi.org/10.1021/ef800001n
  21. Petersen, M.O., Larsen, J., Thomsen, M.H. 2009. Optimization of hydrothermal pretreatment of wheat straw for production of bioethanol at low water consumption without addition of chemicals. Biomass and Bioenergy 33: 834-840. https://doi.org/10.1016/j.biombioe.2009.01.004
  22. Sasaki, C., Okumura, R., Asada, C., Nakamura, Y. 2014. Steam explosion treatment for ethanol production from branches pruned from pear trees by simultaneous saccharification and fermentation. Bioscience, Biotechnology and Biochemistry 78(1): 160-166. https://doi.org/10.1080/09168451.2014.877818
  23. Sheehan, J., Himmel, M.E. 1999. Enzymes, energy, and the environment: Cellulose development in the emerging bioethanol industry. Biotechnology Progress 15 : 817-827. https://doi.org/10.1021/bp990110d
  24. Shevchenko, S.M., Chang, K., Robinson, J., Saddler, J.N. 2000. Optimization of monosaccharide recovery by post-hydrolysis of the water-soluble hemicellulose component after steam explosion of softwood chips. Bioresource Technology 72: 207-211. https://doi.org/10.1016/S0960-8524(99)00125-X
  25. Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D. National Renewable Energy Laboratory, Standard Biomass Analytical Procedures. Available from: www.nrel.gov/biomass/analytical procedures.html (2005).
  26. Sorensen, A., Teller, P.J., Hilstrom, T., Ahring, B.K. 2008. Hydrolysis of Miscanthus for bioethanol production using dilute acid presoaking combined with wet explosion pre-treatment and enzymatic treatment. Bioresource Technology 99: 6602-6607. https://doi.org/10.1016/j.biortech.2007.09.091
  27. Sun, R., Hughes, S. 1998. Fractional extraction and physico-chemical characterization of hemicelluloses and cellulose from sugar beet pulp. Carbohydrate Polymers 36: 293-299. https://doi.org/10.1016/S0144-8617(97)00255-5
  28. Sun, S., Cao, X., Zhang, X., Xu, F., Sun, R., Jones, G. 2014. Characteristics and enzymatic hydrolysis of cellulose-rich fractions from steam exploded and sequentially alkali delignified bamboo (Phyllostachys pubescens). Bioresource Technology 163: 377-380. https://doi.org/10.1016/j.biortech.2014.04.082
  29. Sun, Y.G., Ma, Y.L., Wang, L.Q., Wang, F.Z., Wu, Q.Q., Pan, G.Y. 2015. Physicochemical properties of corn stalk after treatment using steam explosion coupled with acid or alkali. Carbohydrate Polymers 117: 486-493. https://doi.org/10.1016/j.carbpol.2014.09.066
  30. Timilsenaa, Y.P., Abeywickramaa, C.J., Rakshitb, S.K., Brosse, N. 2013. Effect of different pretreatments on delignification pattern and enzymatic hydrolysability of miscanthus, oil palm biomass and typha grass. Bioresource Technology 135: 82-88. https://doi.org/10.1016/j.biortech.2012.09.010
  31. Vanderghem, C., Brostaux, Y., Jacquet, N., Blecker, C., Paquot., M. 2012. Optimization of formic/acetic acid delignification of Miscanthus ${\times}$ giganteus for enzymatic hydrolysis using response surface methodology. Industrial Crops and Products 35: 280-286. https://doi.org/10.1016/j.indcrop.2011.07.014
  32. Yang, B., Boussaid, A., Mansfield, S.D., Gregg, D.J., Saddler, J.N. 2002. Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates. Biotechnology and Bioengineering 77: 678-684. https://doi.org/10.1002/bit.10159
  33. Zhao, X., Cheng, K., Liu, D. 2009. Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis. Applied Microbiology and Biotechnology 82: 815-827. https://doi.org/10.1007/s00253-009-1883-1

Acknowledgement

Grant : Forest Science & Technology Projects

Supported by : Korea Forest Service