자외선B 노출로 인해 손상된 피부세포에 대한 돌배나무잎 추출물의 보호효과

DOI QR코드

DOI QR Code

고아라;최송이;김용웅;박건혁
Koh, Ara;Choi, Songie;Kim, Yong-ung;Park, Gunhyuk

  • 투고 : 2016.01.08
  • 심사 : 2016.03.14
  • 발행 : 2016.03.30

초록

피부 손상은 주로 자외선, 열, 담배 등과 같은 환경적 요인으로부터 초래되는데, 이는 활성산소종의 과생성으로 인한 피부노화와 연관이 있는 것으로 알려져 있다. 돌배(Pyrus pyrifolia NAKAI)는 전 세계적으로 많이 소비되는 과일로써 항암, 항산화, 항염증효과가 알려져 있다. 본 연구에서는 돌배나무잎 추출물(Pyrus pyrifolia leaf extract, PPE)의 ultraviolet B (UVB)스트레스에 대한 피부 섬유아세포 보호효과를 검증하였다. Lactate dehydrogenase assay와 DCF-DA를 이용한 정성분석 실험은 PPE가 인간의 섬유아세포에서 UVB 스트레스에 의해 유발된 세포독성 및 과생성된 활성산소종을 농도 의존적으로 억제할 뿐만 아니라, 미토콘드리아 기능저하, 막전위 저하, 그리고 세포사멸과정의 핵심 인자인 caspase-3 활성도 유의하게 억제함을 보여주었다. 결론적으로, PPE는 UVB스트레스에 의해 과생성된 활성산소종을 억제시켰으며, 이로 인해 생기는 피부세포 사멸을 효과적으로 저해함을 확인하였다.

키워드

Pyrus pyrifolia leaf;dermal fibroblasts cell;antioxidant;mitochondria;antiapoptosis

참고문헌

  1. H. Masaki, Role of antioxidants in the skin: antiaging effects, J. Dermatol. Sci., 58(2), 85 (2010). https://doi.org/10.1016/j.jdermsci.2010.03.003
  2. I. P. Kaur, M. Kapila, and R. Agrawal, Role of novel delivery systems in developing topical antioxidants as therapeutics to combat photoageing, Ageing. Res. Rev., 6(4), 271 (2007). https://doi.org/10.1016/j.arr.2007.08.006
  3. J. Krutmann and P. Schroeder, Role of mitochondria in photoaging of human skin: the defective powerhouse model, J. Investig. Dermatol. Symp. Proc., 14(4), 301 (2009).
  4. M. Brennan, H. Bhatti, K. C. Nerusu, N. Bhagavathula, S. Kang, G. J. Fisher, J. Varani, and J. J. Voorhees, Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin, Photochem. Photobiol., 78(1), 43 (2003). https://doi.org/10.1562/0031-8655(2003)078<0043:MMITMC>2.0.CO;2
  5. H. J. Kim, K. S. Kim, and D. I. Kim, Inhibitory effects of Lespedeza cuneata ethanol extract on ultraviolet-induced photo aging, J. Korean Soc. Food Sci. Nutr., 41(11), 1540 (2012). https://doi.org/10.3746/jkfn.2012.41.11.1540
  6. I. G. Hwang, H. Y. Kim, K. S. Woo, S. H. Lee, J. Lee, and H. S. Jeong, Isolation and identification of the antioxidant DDMP from heated pear (Pyrus pyrifolia NAKAI), Prev. Nutr. Food. Sci., 18(1), 76 (2013). https://doi.org/10.3746/pnf.2013.18.1.076
  7. C. E. Lee, Y. H. Kim, B. G. Lee, and D. H. Lee, Anti-cancer and anti-microbial effect of the fraction isolated from Pyrus ussuriensis Leaves, J. Korean For. Soc., 100(2), 136 (2011).
  8. C. E. Lee, Y. H. Kim, B. G. Lee, and D. H. Lee, Antioxidant effect of the fraction isolated from Pyrus ussuriensis Leaves, J. Korean For. Soc., 99(6), 885 (2010).
  9. J. Y. Cho, C. M. Kim, H. J. Lee, S. H. Lee, J. A. Cho, W. S. Kim, K. H. Park, and J. H. Moon, Caffeoyl triterpenes from pear (Pyrus pyrifolia NAKAI) fruit peels and their antioxidative activities against oxidation of rat blood plasma, J. Agric. Food Chem., 61(19), 4563 (2013). https://doi.org/10.1021/jf400524b
  10. H. W. Kim, K. Y. Kim, B. J. Kim, S. Y. Chiang, Y. H. Do, N. L. Nam, H. W. Jeong, J. S. Choi, and S. J. Cho, Effects of alcoholic fermentation extracts from ovary and rind of pear on human Skin, Korea J. Herbol., 24(1), 133 (2009).
  11. H. S. Lee, Effects of the Pears (Pyrus pyrifolia NAKAI) on alcohol detoxification, Sookmyung Women's Univ., Seoul, Korea (2009).
  12. K. H. Lee, J. Y. Cho, H. J. Lee, Y. K. Ma, J. Kwon, S. H. Park, S. H. Lee, J. A. Cho, W. S. Kim, K. H. Park, and J. H. Moon, Hydroxycinnamoylmalic acids and their methyl esters from pear (Pyrus pyrifolia NAKAI) fruit peel, J. Agric. Food Chem., 59(18), 10124 (2011). https://doi.org/10.1021/jf2022868
  13. W. Liu, J. Liu, M. Xie, C. Liu, W. Liu, and J. Wan, Characterization and high-pressure microfluidization-induced activation of polyphenoloxidase from Chinese pear (Pyrus pyrifolia NAKAI), J. Agric. Food Chem., 57(12), 2376 (2009).
  14. G. Park and M. S. Oh, Acceleration of heat shock-induced collagen breakdown in human dermal fibroblasts with knockdown of NF-E2-related factor 2, BMB Rep., 48(2), 467 (2015). https://doi.org/10.5483/BMBRep.2015.48.8.215
  15. G. Park, H. G. Kim, Y. Sim, S. H. Sung, and M. S. Oh, Sauchinone, A lignan from Saururus chinensis, protects human skin keratinocytes against ultraviolet B-induced photoaging by regulating the oxidative defense system, Biol. Pharm. Bull., 36(7), 1134 (2013). https://doi.org/10.1248/bpb.b13-00101
  16. G. Park, H. G. Kim, Y. O. Kim, S. H. Park, S. Y. Kim, and M. S. Oh, Coriandrum sativum L. protects human keratinocytes from oxidative stress by regulating oxidative defense systems, Skin Pharmacol. Physiol., 25(2), 93 (2012). https://doi.org/10.1159/000335257
  17. G. Park, H. G. Kim, S. P. Hong, S. Y. Kim, and M. S. Oh, Walnuts (seeds of Juglandis sinensis L.) protect human epidermal keratinocytes against UVB-induced mitochondria-mediated apoptosis through upregulation of ROS elimination pathways, Skin Pharmacol. Physiol., 27(3), 132 (2014). https://doi.org/10.1159/000354917
  18. S. H. Seo, G. S. Bae, S. B. Choi, I. J. Jo, D. G. Kim, J. Y. Shin, H. J. Song, S. J. Park, and M. O. Choi, The antioxidative and cytoprotective effect of Lonicerae japonicae FLOS water extracts on the ultraviolet(UV)B-induced human HaCaT keratinocytes, Kor. J. Herbology, 29(6), 63 (2014). https://doi.org/10.6116/kjh.2014.29.6.63.
  19. G. Park, D. S. Jang, and M. S. Oh, Juglans mandshurica leaf extract protects skin fibroblasts from damage by regulating the oxidative defense system, Biochem. Biophys. Res. Commun., 421(2), 343 (2013).
  20. I. B. Afanas'ev, Signaling by reactive oxygen and nitrogen species in skin diseases, Curr. Drug. Metab., 11(5), 409 (2010). https://doi.org/10.2174/138920010791526060
  21. J. Krutmann and P. Schroeder, Role of mitochondria in photoaging of human skin: the defective powerhouse model, J. Investig. Dermatol. Symp. Proc., 14(1), 44 (2009). https://doi.org/10.1038/jidsymp.2009.1
  22. G. Park, T. M. Kim, J. H. Kim, and M. S. Oh, Antioxidant effects of the sarsaparilla via scavenging of reactive oxygen species and induction of antioxidant enzymes in human dermal fibroblasts, Environ. Toxicol. Pharmacol., 38(1), 305 (2014). https://doi.org/10.1016/j.etap.2014.06.009
  23. S. Shalini, L. Dorstyn, S. Dawar, and S. Kumar, Old, new and emerging functions of caspases, Cell. Death. Differ., 22(4), 526 (2015). https://doi.org/10.1038/cdd.2014.216
  24. G. Park, Y. Sim, W. Lee, S. H. Sung, and M. S. Oh, Protection on skin aging mediated by antiapoptosis effects of the water lily (Nymphaea Tetragona GEORGI) via reactive oxygen species scavenging in human epidermal keratinocytes, Pharmacology, 97(5-6), 282 (2016). https://doi.org/10.1159/000444022

과제정보

연구 과제 주관 기관 : 한국연구재단