Culture conditions for mycelial growth of Poria cocos

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Jo, Woo-Sik;Park, Ju-Ri;Oh, So-Ra;Kang, Min-Gu;Kim, Woo-Hyun;Park, Seung-Chun

  • 투고 : 2016.02.29
  • 심사 : 2016.03.24
  • 발행 : 2016.03.31

초록

This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and $35^{\circ}C$, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $FeSO_4{\cdot}7H_2O$ as mineral salts.

키워드

Culture condition;Medicinal mushroom;Poria cocos

참고문헌

  1. Hong IP, Lee MW. 1990. Studies on the cultural characteristics of Poria cocos. Kor. J. Mycol. 18(1): 42-49 (in Korean)
  2. Hong IP, Lee MW, Kim KP, Lee SS. 1991. Studies on the morphology and the mycelial cultivation of Poria cocos (Fr.) Wolf. Kor. J. Mycol. 19(1): 54-60 (in Korean)
  3. Jo WS. 2013. The cultivation methods of Poria cocos and its commercialization. Mushroom Newsletter. 17(2): 59-66 (in Korean)
  4. Jo WS, Yoo YB, Hong IP, Kim DG. 2013. Changes of the cultivation methods of Poria cocos and its commercialization. J Mushroom Sci Prod. 11(4): 303-307 (in Korean) https://doi.org/10.14480/JM.2013.11.4.303
  5. Kirk PM, Cannon PF, David JC and Stalpers JA. 2001. Ainsworth and Bisby's dictionary of the fungi. 9th edn., CAB International, Wallingford.
  6. Park JJ, Ham HB, Lee MW. 1980. Studies on artificial cultivation of Poria cocos. Kor. J. Mycol. 8(3): 133-142 (in Korean)
  7. Shim JO, Son SG, Kim YH, Lee YS, Lee JY, Lee TS, Lee SS and Lee MW. 1997. The culture conditions affecting the mycelial growth of Grifola umbellata. Kor. J. Mycol. 25(3): 209-218. (in Korean)
  8. Sung JM, Yoo YB, Cha DY. 2000. MUSHROOM SCIENCE. Kyo-Hak Publishing Co. 614p.

과제정보

연구 과제 주관 기관 : KOREA FOREST SERVICE