Culture conditions for mycelial growth of Poria cocos



Jo, Woo-Sik;Park, Ju-Ri;Oh, So-Ra;Kang, Min-Gu;Kim, Woo-Hyun;Park, Seung-Chun

  • 투고 : 2016.02.29
  • 심사 : 2016.03.24
  • 발행 : 2016.03.31


This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and $35^{\circ}C$, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $FeSO_4{\cdot}7H_2O$ as mineral salts.


Culture condition;Medicinal mushroom;Poria cocos


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