In Vitro and In Vivo Antioxidant Activity of Aged Ginseng (Panax ginseng)

  • Chung, Soo Im (Department of Food Science and Nutrition, Brain Korea 21 Plus, Kyungpook National University) ;
  • Kang, Mi Young (Department of Food Science and Nutrition, Brain Korea 21 Plus, Kyungpook National University) ;
  • Lee, Sang Chul (Division of Plant Biosciences, Kyungpook National University)
  • Received : 2016.01.11
  • Accepted : 2016.02.05
  • Published : 2016.03.31


Fresh ginseng roots were aged in an oven at $80^{\circ}C$ for 14 d. The in vitro and in vivo antioxidant activities of this aged ginseng, in comparison with those of the white and red ginsengs, were evaluated. In in vitro antioxidant assays, the ethanolic extracts from aged ginseng showed significantly higher free radical scavenging activity and reducing power than those of the white and red ginsengs. In in vivo antioxidant assays, mice were fed a high fat diet supplemented with white, red, or aged ginseng powders. High fat feeding resulted in a significant increase in lipid peroxidation and a substantial decrease in antioxidant enzymes activities in the animals. However, diet supplementation of ginseng powders, particularly aged ginseng, markedly reduced lipid peroxidation and enhanced the antioxidant enzymes activities. The results illustrate that the aged ginseng has greater in vitro and in vivo antioxidant capacity than the white and red ginsengs. The aged ginseng also showed considerably higher total saponin, phenolic, and flavonoid contents, indicating that its antioxidant capacity may have been partly due to its high levels of antioxidant compounds. This new ginseng product may be useful as a functional food with strong antioxidant potential.


Supported by : Rural Development Administration, National Research Foundation of Korea


  1. Tang W, Eisenbrand G. 1992. Panax ginseng C.A. Mey. In Chinese Drugs of Plant Origin: Chemistry, Pharmacology, and Use in Traditional and Modern Medicine. Tang W, Eisenbrand G, eds. Springer-Verlag GmbH, Heidelberg, Germany. p 711-737.
  2. Li X, Yan YZ, Jin X, Kim YK, Uddin MR, Kim YB, Bae H, Kim YC, Lee SW, Park SU. 2012. Ginsenoside content in the leaves and roots of Panax ginseng at different ages. Life Sci J 9: 679-683.
  3. Soldati F, Tanaka O. 1984. Panax ginseng: relation between age of plant and content of ginsenosides. Planta Med 50: 351-352.
  4. Hong YJ, Kim N, Lee K, Sonn CH, Lee JE, Kim ST, Baeg IH, Lee KM. 2012. Korean red ginseng (Panax ginseng) ameliorates type 1 diabetes and restores immune cell compartments. J Ethnopharmacol 144: 225-233.
  5. Lee H, Park D, Yoon M. 2013. Korean red ginseng (Panax ginseng) prevents obesity by inhibiting angiogenesis in high fat diet-induced obese C57BL/6J mice. Food Chem Toxicol 53: 402-408.
  6. Cui X, Sakaguchi T, Ishizuka D, Tsukada K, Hatakeyama K. 1998. Orally administered ginseng extract reduces serum total cholesterol and triglycerides that induce fatty liver in 66% hepatectomized rats. J Int Med Res 26: 181-187.
  7. Hong SH, Suk KT, Choi SH, Lee JW, Sung HT, Kim CH, Kim EJ, Kim MJ, Han SH, Kim MY, Baik SK, Kim DJ, Lee GJ, Lee SK, Park SH, Ryu OH. 2013. Anti-oxidant and natural killer cell activity of Korean red ginseng (Panax ginseng) and urushiol (Rhus vernicifera Stokes) on non-alcoholic fatty liver disease of rat. Food Chem Toxicol 55: 586-591.
  8. Voces J, Alvarez AI, Vila L, Ferrando A, Cabral de Oliveira C, Prieto JG. 1999. Effects of administration of the standardized Panax ginseng extract G115 on hepatic antioxidant function after exhaustive exercise. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 123: 175-184.
  9. Chung IM, Kim JW, Seguin P, Jun YM, Kim SH. 2012. Ginsenosides and phenolics in fresh and processed Korean ginseng (Panax ginseng C.A. Meyer): effects of cultivation location, year, and storage period. Food Chem 130: 73-83.
  10. Sohn SH, Kim SK, Kim YO, Kim HD, Shin YS, Yang SO, Kim SY, Lee SW. 2013. A comparison of antioxidant activity of Korean white and red ginsengs on $H_2O_2$-induced oxidative stress in HepG2 hepatoma cells. J Ginseng Res 37: 442-450.
  11. Bae HJ, Chung SI, Lee SC, Kang MY. 2014. Influence of aging process on the bioactive components and antioxidant activity of ginseng (Panax ginseng L.). J Food Sci 79: H2127-H2131.
  12. Jia L, Zhao Y. 2009. Current evaluation of the millennium phytomedicine-ginseng (I): Etymology, pharmacognosy, phytochemistry, market and regulations. Curr Med Chem 16: 2475-2484.
  13. Lee MR, Yun BS, Sung CK. 2012. Comparative study of white and steamed black Panax ginseng, P. quinquefolium, and P. notoginseng on cholinesterase inhibitory and antioxidative activity. J Ginseng Res 36: 93-101.
  14. Kim MH, Hong HD, Kim YC, Rhee YK, Kim KT, Rho J. 2010. Ginsenoside changes in red ginseng manufactured by acid impregnation treatment. J Ginseng Res 34: 93-97.
  15. Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46: 4113-4117.
  16. Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64: 555-559.
  17. Hatano T, Kagawa H, Yasuhara T, Okuda T. 1988. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem Pharm Bull 36: 2090-2097.
  18. Oyaizu M. 1986. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr Diet 44: 307-315.
  19. American Institute of Nutrition. 1977. Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. J Nutr 107: 1340-1348.
  20. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358.
  21. Hulcher FH, Oleson WH. 1973. Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. J Lipid Res 14: 625-631.
  22. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
  23. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474.
  24. Aebi H. 1974. Catalase. In Method of Enzymatic Analysis. Bergmeyer HU, ed. Academic Press, New York, NY, USA. Vol 2, p 673-684.
  25. Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70: 158-169.
  26. Mize CE, Langdon RG. 1952. Hepatic glutathione reductase. I. Purification and general kinetic properties. J Biol Chem 237: 1589-1595.
  27. Mackness MI, Arrol S, Durrington PN. 1991. Paraoxonase prevents accumulation of lipoperoxides in low-density lipoprotein. FEBS Lett 286: 152-154.
  28. Hwang EY, Kong YH, Lee YC, Kim YC, Yoo KM, Jo YO, Choi SY. 2006. Comparison of phenolic compounds contents between white and red ginseng and their inhibitory effect on melanin biosynthesis. J Ginseng Res 30: 82-87.
  29. Lee SM, Bae BS, Park HW, Ahn NG, Cho BG, Cho YL, Kwak YS. 2015. Characterization of Korean red ginseng (Panax ginseng Meyer): history, preparation method, and chemical composition. J Ginseng Res 39: 384-391.
  30. MacDonald-Wicks LK, Wood LG, Garg ML. 2006. Methodology for the determination of biological antioxidant capacity in vitro: a review. J Sci Food Agric 86: 2046-2056.
  31. Moon JK, Shibamoto T. 2009. Antioxidant assays for plant and food components. J Agric Food Chem 57: 1655-1666.
  32. Niki E. 2010. Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med 49: 503-515.
  33. Kang KS, Kim HY, Pyo JS, Yokozawa T. 2006. Increase in the free radical scavenging activity of ginseng by heat-processing. Biol Pharm Bull 29: 750-754.
  34. Kang KS, Yamabe N, Kim HY, Okamoto T, Sei Y, Yokozawa T. 2007. Increase in the free radical scavenging activities of American ginseng by heat processing and its safety evaluation. J Ethnopharmacol 113: 225-232.
  35. Ibrahim W, Lee US, Yeh CC, Szabo J, Bruckner G, Chow CK. 1997. Oxidative stress and antioxidant status in mouse liver: effects of dietary lipid, vitamin E and iron. J Nutr 127: 1401-1406.
  36. Reiter RJ, Tan DX, Burkhardt S. 2002. Reactive oxygen and nitrogen species and cellular and organismal decline: amelioration with melatonin. Mech Ageing Dev 123: 1007-1019.
  37. Mullineaux PM, Creissen GP. 1997. Glutathione reductase: regulation and role in oxidative stress. In Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Scandalios JG, ed. Cold Spring Harbor Laboratory Press, New York, NY, USA. p 667-713.
  38. Ng CJ, Shih DM, Hama SY, Villa N, Navab M, Reddy ST. 2005. The paraoxonase gene family and atherosclerosis. Free Radic Biol Med 38: 153-163.