Isolation and Characterization of the Plant Growth Promoting Rhizobacterium, Arthrobacter scleromae SYE-3 on the Yam Growth

식물성장촉진근권미생물 Arthrobacter scleromae SYE-3의 분리 및 Yam (Dioscorea japonica Thunb.) 성장에 미치는 영향 연구

  • Hong, Sun Hwa (Department of Environmental and Energy Engineering, The University of Suwon) ;
  • Kim, Ji Seul (Department of Environmental and Energy Engineering, The University of Suwon) ;
  • Sim, Jun Gyu (Department of Environmental and Energy Engineering, The University of Suwon) ;
  • Lee, Eun Young (Department of Environmental and Energy Engineering, The University of Suwon)
  • 홍선화 (수원대학교 환경에너지공학과) ;
  • 김지슬 (수원대학교 환경에너지공학과) ;
  • 심준규 (수원대학교 환경에너지공학과) ;
  • 이은영 (수원대학교 환경에너지공학과)
  • Received : 2015.12.14
  • Accepted : 2016.03.03
  • Published : 2016.03.31


In this study, Arthrobacter scleromae SYE-3, which was isolated from indigenous plant in a subtropical region, Neigeria, with plant growth promoting activity was evaluated to determine the optimal culture condition. A bacterial strain SYE-3 had the IAA productivity ($89.15{\pm}0.36mg/L$) and ACC deaminase activity ($0.20{\pm}0.06$ at 72 hours). Also, optimal culture conditions such as temperature and pH of strain SYE-3 were $20^{\circ}C$ and 10 in LB medium, respectively. Strain SYE-3 had up to 3% salt tolerance in the LB medium. Plant growth promoting ability of strain SYE-3 using yam (Dioscorea japonica Thunb.) was evaluated. As a result, strain SYE-3 had showed very powerful effect on the increase of the shoot length and root biomass of yam (190.0% and 282.41% increase for 112 days, respectively). These results indicated that Arthrobacter scleromae SYE-3 can serve as a promising microbial resource for the biofertilizers of subtropical crops.


ACC;IAA;Plant growth promoting rhizobacteria;Subtropical crops;Yam


Supported by : Korea Ministry of Environment (MOE)


  1. Kloepper, J. W., A. Gutierrez-Estrada, and J. A. Mclnroy (2007) Photoperiod regulates elicitation of growth promotion but not induced resistance by plant growth-promoting rhizobacteria. Can. J. Microbiol. 53: 159-167.
  2. Lugtenberg, B. and F. Kamilova (2009) Plant-growth-promoting rhizobacteria, Annu. Rev. Microbiol. 63: 541-556.
  3. Shaharoona, B., M. Arshad, and A. Khalid (2007) Differential response of etiolated pea seedlings to inoculation with rhizobacteria capable of utilizing 1-aminocyclopropane-1-carboxylate or L-methionine. J. Microbiol. 45: 15-20.
  4. Kohler, J., F. Caravaca, L. Carrasco, and A. Roldan (2007) Interactions between a plant growth-promoting rhizobacterium, an AM fungus and a phosphate-solubilising fungus in the rhizosphere of Lactuca sativa. Appl. Soil Ecol. 35: 480-487.
  5. Chen, Q., H. Y. Hu, M. Gao, J. Xu, Y. Q. Zhou, and J. G. Sun (2011) Screening and identification of a nitrogen fixing bacteria with 1-aminocyclopropane-1-carboxylate deaminase activity. Plant Nutr. Fert. Sci. 17: 1515-1521.
  6. Upadhyay, S. K., D. P. Singh, and R. Saikia (2009) Genetic diversity of plant growth promoting rhizobacteria isolated from rhizospheric soil of wheat under saline condition. Curr. Microbiol. 59: 489-496.
  7. Upadhyay, S. K. and D. P. Singh (2014) Effect of salt-tolerant plant growth-promoting rhizobacteria on wheat plants and soil health in a saline environment. Plant Biology 17: 288-293.
  8. Sachdev, D., V. Agarwal, P. Verma, Y. Shouche, P. Dhakephalkar, and B. Chopade (2008) Assessment of microbial biota associated with rhizosphere of wheat (Triticum aestivum) during flowering stage and their plant growth promoting traits. The Internet Journal of Microbiology 7: 10.5580/21a7.
  9. Khan, A. G. (2005) Role of soil microbes in the rhizospheres of plants growing on trace metal contaminated soils in phytoremediation. J. Trace Elem. Med. Biol. 18: 355-364.
  10. Koo, S. Y. and K. S. Cho (2006) Interaction between plants and rhizobacteria in phytoremediation of heavy metal-contaminated soil. Kor. J. Microbiol. Biotechnol. 2: 83-93.
  11. Nehl, D. B., S. J. Allen, and J. F. Brown (1996) Deleterious rhizosphere bacteria an intergrating perspective (review). Appl. Soil Ecol. 5: 1-20.
  12. Glick, B. R. (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol. 41: 109-117.
  13. Johnson, D. L., D. R. Anderson, and S. P. McGrath (2005) Soil microbial response during the phytoremediation of a PAH contaminated soil. Soil Biol. Biochem. 37: 2334-2336.
  14. Golubev, S. N., A. V. Schelud’ko, A. Y. Muratova, O. E. Makarov, and O. V. Turkovskaya (2009) Assessing the potential of rhizobacteria to survive under phenanthrene pollution. Water Air Soil Poll. 198: 5-16.
  15. So, J. H., D. J. Kim, J. H. Shin, C. B. Yu, and I. K. Rhee (2009) Isolation and characterization of Bacillus cereus A-139 producing auxin from east coast sand dunes. Kor. J. Environ. Agr. 28: 447-452.
  16. Schnoor, J. L. (1997) Phytoremediation: Technology Evaluation Report. TE-98-01. Groundwater remediation technologies analysis center, Iowa City, Iowa, USA.
  17. Ahn, J. H., K. H. Son, H. Y. Sohn, and S. T. Kwon (2005) In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. J. Plant Biotechnol. 32: 217-223.
  18. Ahn, J. W. and J. Y. Yoon (2008) Quality characteristics of noodles added with Dioscorea japonica powder. Korean J. Food Sci. Technol. 40: 528-533.
  19. Ryu, H. Y., Y. S. Kim, S. J. Park, B. H. Lee, S. T. Kwon, and H. Y. Sohn (2006) Isolation and characterization of yam putrefactive psychrotrophic bacteria from rotted yam. Kor. J. Microbiol. Biotechnol. 34: 109-114.
  20. Kwon, C. S., H. Y. Sohn, S. H. Kim, J. H. Kim, K. H. Son, J. S. Lee, J. K. Lim, and J. S. Kim (2003) Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem. 67: 1451-1456.
  21. Ha, Y. D., S. P. Lee, and Y. G. Kwak (1998) Removal of heavy metal and ACE inhibition of Yam mucilage. J. Korean Soc. Food Sci. Nutr. 27: 751-755.
  22. Lee, J. G. (2014) Antioxidant activities and monacolin K production on solid-state fermentation of diverse Yam by Aspergillus species strain. Kor. J. Microbiol. 50: 53-59.
  23. Hong, S. H. and E. Y. Lee (2014) Vegetation restoration and prevention of coastal sand dunes erosion using ion exchange resins and the plant growth-promoting rhizobacteria Bacillus sp. SH1RP8 isolated from indigenous plants. Int. Biodeter. Biodegr. 95: 262-269.
  24. Dell’Amico, E., L. Cavalca, and V. Andreoni (2005) Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiol. Ecol. 52: 153-162.
  25. Hong, S. H., H. W. Ryu, J. Kim, and K. S. Cho (2011) Rhizoremediation of diesel-contaminated soil using the plant growth-promoting rhizobacterium Gordonia sp. S2RP-17. Biodegradation 22: 593-601.
  26. Ma, Y., M. Rajkumar, and H. Freitas (2009) Isolation and characterization of Ni mobilizing PGPB from serpentine soils and their potential in promoting plant growth and Ni accumulation by Brassica spp. Chemosphere 75: 719-725.
  27. Xie, H., J. J. Pasternak, and B. R. Glick (1996) Isolation and characterization of mutants of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 that overproduce indoleacetic acid. Curr. Microbiol. 32: 67-71.
  28. Arora, P. K. and H. Bae (2014) Identification of new metabolites of bacterial transformation of indole by gas chromatography-mass spectrometry and high performance liquid chromatography. Int. J. Anal. Chem. 2014: 1-5.
  29. Banerjee, S., R. Palit, C. Sengupta, and D. Standing (2010) Stress induced phosphate solubilization by Arthrobacter sp. and Bacillus sp. isolated from tomato rhizosphere. Aust. J. Crop Sci. 4: 378-383.
  30. Forni, C., J. Riov, M. G. Caiolai, and E. Tel-Or (1992) Indole-3-acetic acid (IAA) production by Arthrobacter species isolated from Azolla. J. Gen. Microbiol. 138: 377-381.
  31. Pennazio, S. and P. Roggero (1992) Effect of Cd and nickel on ethylene biosynthesis in soybean. Biol. Planta. 34: 345-349.
  32. Glick, B. R. (2003) Phytoremediation: synergistic use of plants and bacteria to clean up the environment. Biotechnol. Adv. 21: 383-393.
  33. Belimov, A. A., V. I. Safronova, T. A. Sergeyeva, T. N. Egorova, V. A. Matveyeva, V. E. Tsyganov, A. Y. Borisov, I. A. Tikhonovich, C. Kluge, A. Preisfeld, K.-J. Dietz, and V. V. Stepanok (2001) Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase. Can. J. Microbiol. 47: 642-652.
  34. Tiwari, S., P. Singh, R. Tiwari, K. K. Meena, M. Yandigeri, D. P. Singh, and D. K. Arora (2011) Salt-tolerant rhizobacteria-mediated induced tolerance in wheat (Triticum aestivum) and chemical diversity in rhizosphere enhance plant growth. Biol. Fertil. Soils 47: 907-916.
  35. Barnawal, D., N. Bharti, D. Maji, C. S. Chanotiya, and A. Kalra (2014) ACC deaminase-containing Arthrobacter protophormiae induces NaCl stress tolerance through reduced ACC oxidase activity and ethylene production resulting in improved nodulation and mycorrhization in Pisum sativum. J. Plant Physiol. 171: 884-894.
  36. Lee, M. J., T. H. Kim, B. Min, and S. J. Hwang (2012) Sodium ($Na^+$) concentration effects on metabolic pathway and estimation of ATP use in dark fermentation hydrogen production through stoichiometric analysis, J. Environ. Manage. 108: 22-26.
  37. Madigan, M. T., J. M. Martinko, P. V. Dunlap, and D. P. Clark (2009) Brock biology of microorganisms. 12th ed., pp. 41-45. Pearson Education, Benjamin Cummings, NY, USA.
  38. Lee, E. Y. and S. H. Hong (2013) Assessment of the changes in the microbial community in alkaline soils using biolog ecoplate and DGGE. KSBB J. 28: 275-281.
  39. Aslantas, R., R. Cakmakci, and F. Sahin (2007). Effect of plant growth promoting rhizobacteria on young apple tree growth and fruit yield under orchard conditions. Sci. Hortic. 111: 371-377.
  40. Nezarat, S. and A. Gholami (2009) Screening plant growth promoting rhizobacteria for improving seed germination, seedling growth and yield of maize, Pakistan. J. Biol. Sci. 12: 26-32.

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