Optimization of shoot cultures and bioactive compound accumulation in Rosa rugosa during acclimatization

Jang, Hae-Rim;Park, Byung-Jun;Park, Seung-A;Pee, Ok-Ja;Park, So-Young;Paek, Kee-Yoeup

  • 투고 : 2016.03.15
  • 심사 : 2016.03.22
  • 발행 : 2016.03.31


Rosa rugosa is a medicinal, ornamental, and edible plant native to Eastern Asian countries, including Korea, Japan, and China. The aim of this study was to establish a system for biomass production and secondary metabolite accumulation during in vitro culture and acclimatization of Rosa rugosa. The highest rate of multiple shoot proliferation was achieved with $8.8{\mu}M$ benzyladenine (BA) (83.3%). However, the number of shoots (14.4 per explant) at $4.4{\mu}M$ BA was higher than that at $8.8{\mu}M$ BA. Compared to BA, a combination of thidiazuron (TDZ) and indole butyric acid (IBA) exhibited significantly lower shoot induction, with only 50.0~79.2% and 4.2~16.7% relative shoot formation, respectively. During acclimatization, shoots were sampled every week and their total phenolic contents were analyzed. Among various growth factors, fresh weight showed the most dramatic increase from the 3rd week (88.0 mg/plant) to 4th week (132.7 mg/plant). Total phenolics and flavonoids contents were the highest at $1^{st}$ week of acclimatization. Depending on developmental stages, total phenolics and flavonoids contents were higher in 1-yr-old shoots grown ex vitro than in those of older field-grown or in vitro-grown plants. Amongst different ages of field grown plants, 6-year-old plants, the oldest in this study, showed the lowest content in total phenolics.


Total phenolics;Flavonoids;Acclimatization;Thidiazuron;Multiple shoot


  1. Alfermann AW, Petersen M. 1995. Natural product formation by plant cell biotechnology. Plant Cell Tiss Organ Cult 43:199-205
  2. Altiner D, Kilicgun H. 2008. The antioxidant effect of Rosa rugosa. Drug Metabol Drug Interact 23:323-327
  3. Benjamin BD, Roja P, Heble MR, Chadha MS. 1987. Multiple shoot cultures of Atropa belladonna: effect of physicochemical factors on growth and alkaloid formation. J Plant Nutr 129:129-135
  4. Dai J, Mumper RJ. 2010. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7313-7352
  5. Dandin VS, Murthy HN. 2012. Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants. Acta Physiol Plant 34:1381-1386
  6. Debnath SC. 2009. Zeatin and TDZ-induced shoot proliferation and use of bioreactor in clonal propagation of medicinal herb, rose root (Rhodiola rosea L). J Plant Biochem Biotechnol 18:245-248
  7. Distabanjong K, Geneve RL. 1997. Multiple shoot formation from cotyledonary node segments of Eastern redbud. Plant Cell Tiss Org Cult 47:247-254
  8. Folin O, Ciocalteu V. 1927. On trysonic and tryptophane determination in proteins. J Bio Chem 27:627-650
  9. Hagendoorn MJM, van der Plas LHW, Segers GJ. 1999. Accumulation of antraquinones in Morinda citrifolia cell suspensions. A model system for the study of the interaction between secondary and primary metabolism, Plant Cell Tiss Org Cult 38: 227-234
  10. Hashidoko Y. 1996. The phytochemistry of Rosa rugosa. Phytochemistry 43:535-549
  11. Ibrahim R, Debergh PC. 2001. Factors controlling high efficiency adventitious bud formation and plant regeneration from in vitro leaf explants of roses (Rosa hybrida L.). Scientia Hort 88:41-57
  12. Jain N, Sharma V, Ramawat KG. 2012. Shoot culture of Bacopa monnieri: standardization of explant, vessels and bioreactor for growth and antioxidant capacity. Physiol Mol Biol Plants 18:185-190
  13. Khan MY, Aliabbas A, Kumar V, Rajkumar S. 2009. Recent advances in medicinal plant biotechnology. Indian J Biotechnol 8:9-22
  14. Lal N, Ahuja PS. 1996. Plantlet regeneration from callus in Picrorhiza kurroa Royle ex Benth. - an endangered medicinal plant. Plant Tiss Cult 6:127-134
  15. Luczkiewicz M, Cisowski W. 2001. Optimisation of the second phase of a two phase growth system for anthocyanin accumulation in callus cultures of Rudbeckia hirta. Plant Cell Tiss Org Cult 65:57-68
  16. Neelakandan AK, Wang K. 2012. Recent progress in the understanding of tissue cultureinduced genome level changes in plants and potential applications. Plant Cell Rep 31:597-620
  17. Paek KY, Chakrabarty D, Hahn EJ. 2005. Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tiss Org Cult 81:287-300
  18. Park SY, Ahn JK, Lee WY, Murthy HN, Paek KY. 2005. Mass production of Eleutherococcus koreanum plantlets via somatic embryogenesis from root cultures and accumulation of eleutherosides in regenerants. Plant Sci 168:1221-1225
  19. Park SY, Bae EK, Moon HK, Lee H, Kim YW. 2011. Physiological changes and gene expression dynamic during somatic embryogenesis of Kalopanax septemlobus. Hort Environ Biotechnol 52:74-82
  20. Praveen N, Naik PM, Manohar SH, Nayeem A, Murthy HN. 2009. In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A. Acta Physiol Plant 31:723-728
  21. Santos-Gomes PC, Seabra RM, Andrade PB, Fernandes-Ferreira M. 2002. Phenolic antioxidant compounds produced by in vitro shoots of sage (Salvia officinalis L.). Plant Sci 162:981-987
  22. Siddique I, Anis M. 2007. In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.):a medicinal plant. Acta Physiol Plant 29:233-238
  23. Takayama S, Misawa M. 1981. Mass propagation of Begonia hiemalis plantlets by shake culture. Plant Cell Physiol 22:461-467
  24. Wu CH, Dewir YH, Hahn EJ, Paek KY. 2006. Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193-199
  25. Youwei Z, Yonghong P. 2007. Changes in antioxidant activity in Rosa rugosa flowers at different stages of development. NZ J Crop Hortic Sci 35:397-401

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연구 과제 주관 기관 : Chungbuk National University