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Antioxidant Effects of Eriodictyol on Hydrogen Peroxide-Induced Oxidative Stress in HepG2 Cells

산화스트레스가 유도된 HepG2 세포에서 Eriodictyol의 항산화 효과

  • Joo, Tae-Woo (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University) ;
  • Hong, Sung-Hyun (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University) ;
  • Park, Sun-Young (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University) ;
  • Kim, Gur-Yoo (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University) ;
  • Jhoo, Jin-Woo (Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University)
  • 주태우 (강원대학교 동물생명과학대학 축산식품과학전공) ;
  • 홍성현 (강원대학교 동물생명과학대학 축산식품과학전공) ;
  • 박선영 (강원대학교 동물생명과학대학 축산식품과학전공) ;
  • 김거유 (강원대학교 동물생명과학대학 축산식품과학전공) ;
  • 주진우 (강원대학교 동물생명과학대학 축산식품과학전공)
  • Received : 2016.02.15
  • Accepted : 2016.04.04
  • Published : 2016.04.30

Abstract

This study was conducted to investigate the antioxidant and hepatoprotective effects of eriodictyol compound against hydrogen peroxide-induced oxidative stress in HepG2 cells by measuring expression levels of antioxidant enzymes, liver function index enzyme activities, and inhibitory effects against reactive oxygen species (ROS) production. HepG2 cell viability was assessed using 3-(4,5-dimethyl thiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. In the concentration range of $10{\sim}50{\mu}g/mL$, eriodictyol displayed over 98% cell viability in HepG2 cells. The effects of increased gene expression on hydrogen peroxide-induced oxidative stress were analyzed by monitoring antioxidant enzyme (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx) gene expression levels using real-time PCR. Eriodictyol compound significantly increased gene expression levels of SOD, CAT, and GPx in a dose-dependent manner ($10{\sim}50{\mu}g/mL$). Hepatoprotective effects against hydrogen peroxide-induced oxidative stress were analyzed by monitoring glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) activities in HepG2 cell culture medium using a biochemistry analyzer. Eriodictyol compound significantly reduced GOT, LDH, and GGT activities in a dose-dependent manner in HepG2 cells. ROS level in HepG2 cells was analyzed by 2',7'-dichlorofluorescein fluorescence diacetate assay, and eriodictyol compound effectively reduced the intracellular ROS level in HepG2 cells. The results reveal that eriodictyol compound can be useful for development of effective antioxidant and hepatoprotective agents.

Acknowledgement

Supported by : 강원대학교

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