천연제오라이트 세라믹볼을 이용한 곤충병원성 곰팡이 포자 생산 방법

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이중복;김범수;주우홍;권기석
Lee, Jung-Bok;Kim, Beaum-Soo;Joo, Woo-Hong;Kwon, Gi-Seok

  • 투고 : 2015.10.12
  • 심사 : 2016.03.26
  • 발행 : 2016.03.31

초록

본 곤충병원성곰팡이 B. bassiana M130의 포자를 대량생산하기 위한 방안으로 천연제오라이트 세라믹볼, 합성제오라이트 세라믹볼과 활성탄볼을 이용한 담체배양을 실시하였다. 그 중 천연제오라이트 세라믹볼 이용한 포자생산방법이 가장 우수 한 것으로 조사 되었으며, 배양 최적조건은 미강추출물(1:8, W/V) 3 mL, 전분 33 g, 포자현탁액 7 mL, pH3과 NaCl 5%로 배양하였을 때 B. bassiana M130 포자를 4.2×108 conidium/mL 생산하고, 사이클론 회수방법을 통해 순수포자만 63%의 회수율을 보였다. 향후 천연제오라이트 세라믹볼을 이용한 곤충병원성 곰팡이 B. bassiana M130 포자의 대량생산이 가능하며, 온실가루이제어용 친환경살충제로 제품생산 및 원제의 대량생산을 통해 국산화 가능성을 제안하고자 한다.

키워드

Biological control;Ceramic ball;Entomopathogenic fungi;Rice bran;Spore

참고문헌

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과제정보

연구 과제번호 : (온실/담배)가루이 방제를 위한 곤충병원성 곰팡이를 활용한 미생물살충제의 산업화 기술

연구 과제 주관 기관 : 안동대학교 산학협력단