- Volume 32 Issue 1
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Safety evaluation of cricket(Gryllus bimaculatus) extract in Sprague-Dawley rats
- Lee, Somin ;
- Ahn, Kyu Sup ;
- Ryu, Hyeon Yeol ;
- Kim, Hye Jin ;
- Lee, Jin Kyu ;
- Cho, Myung-Haing ;
- Ahn, Mi Young ;
- Song, Kyung Seuk
- Received : 2016.01.04
- Accepted : 2016.03.26
- Published : 2016.03.31
Recently, research investment in the improvement of food safety as a food source and specializing of nutritional source of edible insects is being actively conducted. Cricket especially has been attracting considerable interest in entomophagy; however, research on the safety assessment of cricket is limited. This study investigated the effects of cricket ethanol extract when orally administrated in Sprague-Dawley rats. Here, we performed a 4 wk repeated oral dose toxicity test in Sprague-Dawley rats following the Organization for Economic Cooperation and Development test guidelines 407 under Good Laboratory Practice regulation. Rats were randomly allocated 4 groups: vehicle control, 250, 500, 1,000 mg/kg test groups and administrated based on body weight for 28 d. The animals were observed for mortalities and clinical signs, body weight changes, food and water consumption. At the end of treatment period, blood and urine were collected and analyzed. Subsequently, the animals were sacrificed and subjected to gross pathological examination and organ weight measurement. The organs were preserved for histopathological examination. The results showed that there were no systemic toxicological effects related with the cricket ethanol extract in the 4 wk oral repeated dose toxicity study. It is considered that NOAEL of cricket ethanol extract is greater than 1,000 mg/kg/d and there was no target organ detected.
cricket;cricket ethanol extract;Gryllus bimaculatus;NOAEL
- Adebowale YA, Adebowale KO, Oguntokun MO (2005) Evaluation of Nutritive Properties of the Large African Cricket (Gryllidae sp.). Pak J Sci Industrial Res 48(4), 274.
- Ahn MY, Ryu KS, Park BY, Kim DW, Kim I, Kim SH (2000) Effects of cricket supplements on the chicken meats and its eggs. Korean J Poult Sci 27(3), 197-202.
- Ahn MY, Bae HJ, Kim IS, Yoo EJ, Kwack SJ, Kim HS et al. (2005) Genotoxic evaluation of the biocomponents of the cricket, Gryllus bimaculatus, using three mutagenicity tests. J Toxicol Environ Health A 68, 2111-2118. https://doi.org/10.1080/15287390500182537
- Ahn MY, Han JW, Kim SJ, Hwang JS, Yun EY (2011) Thirteen-week oral dose toxicity study of G. bimaculatus in Sprague-Dawley rats. Toxicol Res 27(4), 231. https://doi.org/10.5487/TR.2011.27.4.231
- Belluco S, Losasso C, Maggioletti M, Alonzi CC, Paoletti MG, Ricci A (2013) Edible insects in a food safety and nutritional perspective: a critical review. Compr Rev Food Sci Food Saf 12(3), 296-313. https://doi.org/10.1111/1541-4337.12014
- Ahn MY, Han JW, Hwang JS, Yun EY, Lee BM (2014) Antiinflammatory effect of glycosaminoglycan derived from gryllus bimaculatus (A Type of Cricket, Insect) on adjuvant-treated chronic arthritis rat model. J Toxicol Environ Health A 77(22-24), 1332-1345. https://doi.org/10.1080/15287394.2014.951591
- Ahn MY, Hwang JS, Yun EY, Kim MJ, Park KK (2015a) Anti-aging effect and gene expression profiling of aged rats treated with G. bimaculatus extract. Toxicol Res 31(2), 173. https://doi.org/10.5487/TR.2015.31.2.173
- Ahn MY, Kim MJ, Kwon RH, Hwang JS, Park KK (2015b) Gene expression profiling and inhibition of adipose tissue accumulation of G. bimaculatus extract in rats on high fat diet. Lipids Health Dis 14(1), 116. https://doi.org/10.1186/s12944-015-0113-3
- Belluco S, Losasso C, Maggioletti M, Alonzi C, Ricci A, Paoletti MG (2015) Edible insects: a food security solution or a food safety concern. Anim Front 5(2), 25-30. https://doi.org/10.2527/af.2015-0005
- FAO (2011) Codex alimentarius commission E. development of regional standard for edible crickets and their products. FAO, Rome, Italy.
- Finke MD (2002) Complete nutrient composition of commercially raised invertebrates used as food for insectivores. Zoo Biol 21(3), 269-285. https://doi.org/10.1002/zoo.10031
- Hamerman EJ (2015) Cooking and disgust sensitivity influence preference for attending insect-based food events. Appetite 96, 319-326. https://doi.org/10.1016/j.appet.2015.09.029
- Ismasyahir AR, Yusof HA, Engku AEA (2012) Nutritional evaluation of house cricket (Brachytrupes portentosus) meal for poultry. Pak J Sci Res 48(4), 274-278.
- OECD (2008) OECD Guidelines for the Testing of Chemicals, Section 4, Test no. 407 [Internet] Available from http://www.oecd-ilibrary.org/environment/oecd-guidelines-for-the-testing-of-chemicals_chem_guide_pkg-en. [accessed on 27 October 2014]
- Jiang Q (2014) Natural forms of vitamin E: metabolism, antioxidant, and anti-inflammatory activities and their role in disease prevention and therapy. Free Radic Biol Med 72, 76-90. https://doi.org/10.1016/j.freeradbiomed.2014.03.035
- Kim EM, Lim JH, Chang YJ, Ah SH, Ahn MY (2015) Changes in the quality characteristics of cricket (Gryllus bimaculatus) under various processing conditions. Korean J Food Preserv 22(2), 218-224. https://doi.org/10.11002/kjfp.2015.22.2.218
- Kim HS, Jung CU (2013) Nutritional Characteristics of Edible Insects as Potential Food Materials. Korean J Apic 28(1), 1-8.
- Oonincx DGAB, van Itterbeeck J, Heetkamp MJ, van den Brand H, van Loon JJ, van Huis A (2010) An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption. PLoS One 5(12). https://doi.org/10.1371/journal.pone.0014445
- Park KT (2001) Insect resources. p 202, World Science publishing company, Seoul, Korea.
- Premalatha M, Abbasi T, Abbasi SA (2011) Energy-efficient food production to reduce global warming and ecodegradation: The use of edible insects. Renew Sustain Energy Rev 15(9), 4357-4360. https://doi.org/10.1016/j.rser.2011.07.115
- Ross AC (2011) The 2011 report on dietary reference intakes for calcium and vitamin D. Public Health Nutr 14(5), 938-939. https://doi.org/10.1017/S1368980011000565
- Rumpold BA, Schlüter O (2015) Insect-based protein sources and their potential for human consumption: Nutritional composition and processing. Anim Front 5(2), 20-24.
- Sampath H, Ntambi JM (2005) Polyunsaturated fatty acid regulation of genes of lipid metabolism. Annu Rev Nutr 25, 317-340. https://doi.org/10.1146/annurev.nutr.25.051804.101917
- Sung JH, Park SJ, Jeong MS, Song KS, Ahn KS, Ryu HR et al. (2014) Physicochemical analysis and repeated-dose 90-days oral toxicity study of nanocalcium carbonate in Sprague-Dawley rats. Nanotoxicology. 9(5), 603-12. https://doi.org/10.3109/17435390.2014.958587
- UN (2013) World population prospects: The 2012 Revision. UN, New York, NY, USA.
- van Huis VA, Itterbeeck VJ, Klunder H, Mertens E, Halloran A, Muir G et al. (2013) Edible insects: future prospects for food and feed security. pp. 67-68, FAO, Rome, Italy.
- van Huis A, Dicke M, van Loon JJA (2015) Insects to feed the world. J Insect Food Feed 1(1), 3-5. https://doi.org/10.3920/JIFF2015.x002
- Wang D, Bai YY, Li JH, Zhang CX (2004) Nutritional value of the field cricket (Gryllus testaceus walker). Insect Sci 11(4), 275-283. https://doi.org/10.1111/j.1744-7917.2004.tb00424.x
- Hepatoprotective Effects of Insect Extracts in an Animal Model of Nonalcoholic Fatty Liver Disease vol.10, pp.6, 2018, https://doi.org/10.3390/nu10060735