항균력을 지닌 Bacillus 균주들을 종균으로 사용한 청국장에서 Bacillus cereus 억제

DOI QR코드

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이재용;심재민;류샤오밍;야오좡;이강욱;조계만;김경민;신정혜;김정상;김정환
Lee, Jae Yong;Shim, Jae Min;Liu, Xiaoming;Yao, Zhuang;Lee, Kang Wook;Cho, Kye Man;Kim, Gyoung Min;Shin, Jung-Hye;Kim, Jong-Sang;Kim, Jeong Hwan

  • 투고 : 2016.01.11
  • 심사 : 2016.02.16
  • 발행 : 2016.05.31

초록

유해균인 Bacillus cereus와 독소 생성 곰팡이 증식 억제 능력을 지닌 Bacillus amyloliquefaciens EMD17(EMD17)과 Bacillus subtilis EMD4(EMD4)를 단독 혹은 1:1로 대두에 접종하여 청국장을 제조하였다. 대조구로는 나토균주로 알려진 Bacillus subtilis KACC16450 균을 접종한 청국장을 제조하였다. B. amyloliquefaciens EMD17 접종 청국장에서는 오염시킨 B. cereus 증식이 즉시 억제되어 $37^{\circ}C$ 발효 12시간에 검출되지 않았다. EMD4 접종 청국장에서도 B. cereus 증식 억제가 관찰되었으나 억제 정도는 약했다. 48시간 발효 후 청국장 시료들은 $4^{\circ}C$에서 10일간 저장하였고 B. cereus는 EMD4 접종 청국장에서 검출되지 않았다. 그러나 대조구에서는 상당수 B. cereus 균수가 유지되었다. 청국장의 pH 값은 시료 간 유의차가 없었고 혈전용해능은 발효 중에는 EMD17을 접종한 청국장이 그리고 저장 중에는 나토균주를 접종한 것이 높았다. 총 페놀성 화합물도 나토균주 청국장에서 높았다. 이상 결과들에서 B. amyloliquefaciens EMD17 같은 균주들을 종균으로 사용함으로써 청국장에서 B. cereus 억제가 가능함을 알 수 있다.

키워드

cheonggukjang;starter;Bacillus subtilis;Bacillus amyloliquefaciens

참고문헌

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피인용 문헌

  1. 1. Single Oral Dose Toxicity Test and Four Weeks Repeated Oral Dose Determination Test of Crude Antifungal Compounds Produced by Bacillus subtilis SN7 in Rats vol.27, pp.3, 2016, doi:10.3746/jkfn.2016.45.5.736

과제정보

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