Induction of Growth Inhibition and Apoptosis in Human Cancer Cells by a Brown Algae Extract

갈조류 추출물에 의한 인간 암세포 성장 억제 및 세포 사멸 유도

Choo, Kang-Sik;Lee, Hae-Nim;Shin, Seong-Ah;Kim, Hyeong-Jin;Park, Young-Seok;Kim, Sang-Ki;Jung, Ji-Youn

  • Received : 2015.12.24
  • Accepted : 2016.04.27
  • Published : 2016.05.30


In this study, we investigated the effects of Undaria pinnatifida (UP), Petalonia binghamiae (PB) and Punctaria latifolia (PL) extracts on the inhibition of proliferation and apoptosis in human gastric and breast cancer cells. AGS, MDA-MB-231 and SK-BR-3 cells were treated with 0, 50, 100, and 200 μg/ml concentrations of the extracts to determine their anti-proliferative effects, using the MTT assay. The UP, PB and PL extracts inhibited proliferation of AGS, MDA-MB-231 and SK-BR-3 cells in a dose-dependent manner, and the PL extract was found to be the most effective. DAPI staining was also performed to determine changes in the cell nucleus. Further, the AGS, MDA-MB-231 and SK-BR-3 cells were treated with 0, 50, 100, and 200 μg/ml of only the PL extract. DAPI staining showed increased chromatin condensation, which is indicative of apoptosis, in the 200 μg/ml group. The expression of the Bax, Bcl-2, and PARP proteins in AGS, MDA-MB-231 and SK-BR-3 cells treated with the PL extract was also determined by western blot analysis. The expression of Bax (a pro-apoptotic protein) and cleaved-PARP was increased, whereas the expression of Bcl-2 (an anti-apoptotic protein) was decreased compared with the control. These findings indicate that the PL extract may have potential as an alternative anticancer drug and nutraceutical.


Apoptosis;Bcl-2 family;Petalonia binghamiae;Punctaria latifol;Undaria pinnatifida


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