Effect of Salvia plebeia Water Extract on Antioxidant Activity and Lipid Composition of Rats Fed a High Fat-High Cholesterol Diet

배암차즈기 열수 추출물 첨가가 고지방-고콜레스테롤 식이를 급여한 흰쥐의 항산화 활성과 지질조성에 미치는 영향

  • Received : 2016.03.26
  • Accepted : 2016.05.23
  • Published : 2016.05.31


This study examined the antioxidant activity and lipid composition of rats fed a high fat-high cholesterol diet containing Salvia plebeia water extract. Three levels (50 mg/kg, 200 mg/kg, 500 mg/kg) of Salvia plebeia water extract were administered to seven weeks old male Sprague Dawley rats for four weeks. The activity of glutathione peroxidase in serum was high in all groups that received the Salvia plebeia water extract (p<0.05). The glutathione reductase activity was high (p<0.05) in groups that received 50 mg/kg and 500 mg/kg of Salvia plebeia water extract. When rats received Salvia plebeia water extract, the content of MDA (malondialdehyde) in the serum and liver was low in all groups. In addition, the serum total lipid, triglyceride, total cholesterol and LDL-cholesterol contents decreased significantly (p<0.05). Total cholesterol and triglyceride levels in the liver were significantly lower in the group that received the Salvia plebeia water extract than in the control group (p<0.05). Salvia plebeia water extract improves lipid metabolism and it almost meets with the increase of antioxidant activity and peroxide formation reduction. Based on these results, supplemented of a high fat-hypercholesterol diet with Salvia plebeia can increase antioxidant activity and depress peroxide formation in serum, as well as liver cholesterol and triglycerides.


Supported by : Sangji University


  1. Brenot A, King KY, Janowiak B, Griffith O, Caparon MG(2004) Contribution of glutathione peroxidase to the virulence of Streptococcus pyogenes. Infect Immun 72, 408-413
  2. Byun BH, Seo BI(2003) Effect of Playtycodi radix powder on enzyme activities of obese rats serum fed high fat diet. Korean J Herbol 18(4), 135-139
  3. Bae MJ, Ye EJU, Kim SJ, Kim JM, Yee ST, Park EM(2007) The effects of Plebeiae Herba(Salvia plebeia R. Br.) on the anticancer(in vitro) and activation of immune cells. J Korean Soc Food Sci Nutr 36(4), 377-382
  4. Boo HO, Shin JH, Shin JS, Choung ES, Bang MA, Choi KM, Song WS(2012) Assessment on antioxidant potential and enzyme activity of some economic resource plants. Korean J Plant Res 25(3), 349-356
  5. Boo HO, Shin JW, Choung ES, Bang MA, Choi KM, Song WS(2013) Carbohydrate, lipid inhibitory avtivity and antioxidant activity of extracts from several economic resource plants in vitro. Korean J Plant Res 32(4), 297-301
  6. Bradford MM(1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248
  7. Cho SJ(2012) Local herbe with patent. Academy Book, pp 235-237
  8. Choi YG, Kim SH, Lim JP, Kim DK, Eom DO, Lee KB, Kim SY, Shin TY(2001) Inhibitory effect of Salvia plebeia on compound 48/80-induced immediated hypersensitivity reaction. Korean J Pharmacogn 32(4), 297-301
  9. Folch J, Lees M, Sloane-Stanley G(1957) A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem 226, 497-509
  10. Frings CS, Dunn RT(1970) A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. Am J Clin Pathol 53, 89-91
  11. Han EG, Cho SY(1997) Effect of Codonopsis lancedata water extract on the activities of antioxidative enzyme in carbon tetrachloride treated rats. J Korean Soc Food Sci Nutr 26(6), 1181-1186
  12. Han EG, Sung IS, Moon HK, Cho SY(1998) Effect of Codonopsis lancedata water extract on the level of lipid in rats fed high fat diet. J Korean Soc Food Sci Nutr 27(5), 940-944
  13. Han LK, Xu BJ, Kimura Y, Zheng Y, Okuda H(2000) Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. J Nutr 130(11), 2760-2764
  14. Han LK, Zheng YN, Okuda H, Kimura Y(2002) Saponins from platycodi radix ameliorate high fat diet-induced obesity in mice. J Nutr 132(8), 2241-2245
  15. Han S, Oh KS, Yoon Y, Park JS, Park YS, Han JH, Jeong AL, Lee S, Park M, Choi YA, Lim JS, Yang Y(2011) Herbal extract THI improves metabolic abnormality in mice fed a high-fat diet. Nutr Res Pract 5(3), 198-204
  16. Hirakawa Y, Shimokawa H(2001) Lipid–lowering drugs. Nippon Yakurigaku Zasshi 118(6), 389-395
  17. Jeon MH(2015) Antiobesity effect and antioxidant activities of Momordica charantia ethyl acetate extract. Master's thesis, Sangji University.
  18. Jeong HR, Sung MS Kim YH, Ham HM, Choi, YM, Lee JS(2012) Anti-infammatory activity of Salvia plebeia R. Br. Leaf through heme oxygenase-1 induction in LPS-stimulated RAW264.7 macrophages. J Korean Soc Food Sci Nutr 41(7), 888-894
  19. Jeong JS, Kim YJ, Choi BR, Go GB, Son BG, Gang SW, Moon SM(2014) Antioxidant and physicochemical changes in Salvia plebeia R. Br. after hot-air drying and blanching. J Korean Soc Food Sci Nutr 43(6), 893-900.
  20. Jin CY, Byun BH, Park JH, Lee ES, Choi HY, Lee BW, Seo BI(2002) Effects of Platycodon grandiflorum powder on body weight and serum biological composition of obese rats fed high fat diet. Korean J Herbol 17(2), 19-28
  21. Jo SY, Lee UY, Kim EY, Lee SJ, Her JW, Yoon TJ(2010) A study on the anti-inflammatory and anti-allergic effect of Salvia plebeia extracts. Korean J Pharmacogn 41(1), 31-37
  22. Kang HS, Chung HY, Son KH, Kang SS, Choi JS(2003) Scavenging effect of Korean medicinal plants on the peroxynitric and total ROS. Nat Product Sci 9(2), 73-79
  23. Kim JY, Jung KS, Jeong HG(2004) Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Lett 569, 321-326
  24. Kim SH, Kwon CS, LeeJS, Son KH, Lim JK, Kim JS(2002) Inhibition of carbohydrate-digesting enzyme and amelioration of glucose tolerance by Korean medicinal herbs. J Food Sci Nutr 7(1), 62-66
  25. Kim SO, Lee MY(2001) Effects of ethylacetate fraction of onion on lipid metabolism in high cholesterol-fed rats. J Korean Soc Food Nutr 30(4), 673-678
  26. Koh JB, Choi MA, Kim JY, Rho MH, Kim DJ(1999) Effects of tea fungus/kombucha beverage on serum protein levels and enzyme activity in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 28(5), 1137-1143
  27. Korea National Statistical Office(2014) 20013 Summary report of the cause of death statistics
  28. Lee HS, Choi JH, Kim YE, Kim IH, Kim BH, Lee CH(2013) Effects of the Cynanchum wilfordii ethanol extract on the serum lipid profile in hypercholesterolemic rats Pre Nutr Food Sci 18(3), 157-162
  29. Lim JA, Yun BW, Baek SH(2007) Antioxidative activity and nitrite scavenging ability of methanal extract from Salvia plebeia R. Br Korean J Med Crop Sci 15(3), 183-188
  30. Ministry of Health and Welfare, Korea Center and Prevention(2014) 2013 National health and nutrition examination survey: overview. Ministry of Health and Welfare, Seoul, Korea
  31. Park MH, Son GM, Bae MJ(1995) Effect of Ptatycodi radix and Platycodi radix saponin on liver lipid in rats on a fed high fat diet. Korean J Food Nutr 8(3), 222-229
  32. Park MN(2005) Effect of iron overload during pregnancy and lactation on iron metabolism and oxidative stress in maternal, fetal and neonatal rats. PhD Dissertation, Seoul National University
  33. Shin TY, Kim DK, Choi YK, Kim YJ, Choi DS, Kim SH, An NH(2000) Effect of Salvia plebeia on IgE antibody mediated allergic reaction in rats. Int J Oriental Med 1(2), 29-35
  34. Shin MK, Kim SK, Lee SK, Yang EY, Lee HO, Baek SH(2001) Cytotoxicity and amtimicrobial effect of the extract of Salvia plebeia. Korean J Pharmacogn 32(1), 55-60
  35. Sung TS, Son GM, Bae MJ(1994) Effect of Cnidi rhizoma water-extracted solution on fat contents in plasma, liver, and adipose, and fecal steroids of fatted rats induced by high fat dietary. Korean J Food Nutr 7(2), 100-107
  36. Um SH, Kim DK, Kwak JW, Lee KR, Rhee DK, Pyo SN, Zee OP(1995) Antiviral and antitumoral activities of domestic medicinal plants in macrophages. Korean J Pharmacogn 26(3), 259-264
  37. Um SH, Lee KR, Zee OP, Pyo SN(1996) The effect of Salvia plebeia on murine macrophage-mediated cytoxicity. Nat Product Sci 2(1), 43-47
  38. Won HR, Oh HS(2007) Antioxidative activity and lipid composition from different part and supplement of Codonopsis lanceolata in rat. J Korean Soc Food Sci Nutr 36(9), 1128-1133
  39. Won HR(2012) Effect of Acanthopanax senticosus water extract on lipid metabolism in rats fed hypercholesterol diet. Korean J Community Living Sci 23(4), 501-508
  40. Won HR(2013) Hypocholesterolemic effect of Platycodi radix saponin in rats on a fed hypercholesterol diet. Korean J Community Living Sci 24(1), 141-149
  41. Won HR(2015) Effect of the Officinale Makino water extract on the lipid metabolism of rats on fed a hypercholesterol diet. Korean J Community Living Sci 26(2), 351-360
  42. Won HR, Kim SH(2011) Antihyperlipidemic Effect of ethanol extract from portulaca oleracea high fat diet-induced obese mice. J Korean Soc Food Nutr 40(4), 538-543
  43. Zhao HL, Sim JS, Shim SH, Ha YW, Kang SS, Kim YS(2005) Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction. Int J Obes 29(8), 983-990

Cited by

  1. Effects of Salvia plebeia R. Br. on Antioxidative Enzyme Activities and Oxidative Damage in Rats Fed High-Fat and High-Cholesterol Diets vol.33, pp.4, 2018,