Evaluation of antibacterial and therapeutic effects of egg-white lysozyme against Salmonella Typhimurium in ICR mice infected with Salmonella Typhimurium

난백 유래 라이소자임의 마우스 살모넬라증에 대한 항균 및 치료 효과

  • Kim, Hee-Gyu (Uiryeong High School) ;
  • Hwang, Jae-Seung (Jinju High School) ;
  • Jae, Woo-Young (Jinju High School) ;
  • Son, Song-Ee (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University)
  • Received : 2016.04.01
  • Accepted : 2016.05.17
  • Published : 2016.06.30


Salmonellosis is a major bacterial zoonosis that causes self-limited enteritis in animals and foodborne disease and typhoid fever in humans. Recently, multi-drug-resistant strains of Salmonella spp. have increased and caused more serious problems in public health. The present study investigated the antibacterial effects of egg-white lysozyme (EWL) against Salmonella (S.) Typhimurium and the therapeutic effects of EWL for murine salmonellosis. Evaluation of the antibacterial effects of EWL against S. Typhimurium revealed a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EWL of 6.25 and $300{\mu}g/mL$, respectively. In the bacterial growth inhibition test, EWL at 300 (p < 0.05) and $600{\mu}g/mL$ (p < 0.01) significantly inhibited the growth of S. Typhimurium at 4 h post-incubation. EWL administration at MIC (LYS-1), MBC (LYS-2) and $2{\times}MBC$ (LYS-3) for 14 days resulted in mortality of mice infected with S. Typhimurium of 70, 40 and 10%, respectively, while that of control mice (CON) was 90%. Counts of S. Typhimurium in murine spleens were significantly lower in LYS-2 and LYS-3 than CON (p < 0.05). The results of this study indicate that EWL has the potential for treatment of ICR mice infected with S. Typhimurium.


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