Genetic variation of halophyte New Zealand spinach (Tetragonia tetragonioides) accessions collected in Korea using an AFLP marker

AFLP 마커를 이용한 국내수집 염생식물 번행초 유전다양성 평가

  • Received : 2016.04.29
  • Accepted : 2016.05.22
  • Published : 2016.06.30


This study was conducted to investigate the potential use of New Zealand spinach (Tetragonia tetragonioides) as a new vegetable crop which will be cultivated in salt-affected soils such as reclaimed areas. New Zealand spinach ecotypes native to Korea were collected across the Southern, Western and Eastern seashore regions of the Korean peninsula, among which fifty-five accessions were later further propagated and evaluated genetically by using an AFLP (amplified fragment length polymorphism) marker. Based on the AFLP analysis performed to uncover the genetic diversity of the collected ecotypes, enzymatic cleavage of the extracted DNA was implemented based on 12 EcoRI and MseI combinations. A total of 1,279 alleles (107 alleles per EcoRI and MseI enzyme combination) were successfully amplified, among which 62 alleles per enzyme combination were polymorphic (58%). The AFLP analysis indicated that the rate of genetic dissimilarity was 29% among the New Zealand spinach collections, which were clustered into the 7 genetic diversity group. This is the first report on the genetic variation in the genus Tetragonia, and the basic information can be applied to select parental lines for enhancing the segregation spectrum of the new halophytic vegetable plant grown in salt-affected areas.


Halophyte;Leafy vegetable;New Zealand spinach;Genetic diversity;PCR marker


  1. Rohlf FJ (1989) NTSYS-pc numerical taxonomy and multivariate analysis system, version 1.50. Exeter Publications. New York, USA
  2. Sampaio BL, Bara MTF, Ferri PH, Santos SC, Paula JR (2011) Influence of environmental factors on the concentration of phenolic compounds in leaves of Lafoensia pacari. Revista Brasileira de Farmacognosia 21:1127-1137
  3. Sathyanarayana N, Leelambika M, Mahesh S, Jaheer M (2011) AFLP assessment of genetic diversity among Indian Mucuna accessions. Physiol Mol Biol Plants 17:171-180
  4. Zhang L, Jeon YJ, Kang SY, Lee GJ (2012) Genetic diversity of natural and artificial populations of model grass Brachypodium species evaluated by AFLP markers. Hort. Environ. Biotechnol 53:143-150
  5. Aoki T, Takagi K, Hirata T, Suga T (1982) Two naturally occurring acyclicditerpene and norditerpene aldhydes from Tetragonia tetragonoides. Phytochemistry 21(6):1361-1363
  6. Arroyo-Garcia R, Martinez-Zapater JM, Fernandes Prieto JA (2001) AFLP evaluation of genetic similarity among laurel populations (Laurus L.). Euphytica 122:155-164
  7. Blears MJ, de Grandis SA, Lee H, Trevors JT (1998) Amplified fragment length polymorphism (AFLP): a review of the procedure and its applications. Indian J Microbiol Biotechnol 21:99-114
  8. Choi HJ, Kang JS, Jeong YK, Choi YW, Joo WH (2008) Inhibitory activity on the diabetes related enzymes of Tetragonia tetragonioides. Korean J Biotechnol Bioeng 23(5):419-424
  9. Grubben GJH, Denton OA (2004) Plant resources of tropical Africa 2. Vegetables. Backhuys Publishers, Wageningen, The Netherlands
  10. Kang SY, Lee GJ, Lim KB, Lee HJ, Park IS, Chung SJ, Kim JB, Kim DS, Rhee HK (2008) Genetic diversity among Korean bermudagrass ecotypes characterized by morphological and cytological and molecular approaches. Mol Cell 25:163-171
  11. Keim P, Olson TC, Shoemaker RC (1988) A rapid protocol for isolating soybean DNA. Soybean Genetics Newsletter 15:150-152
  12. Kim JH, SS Park, CK Song (2008) Cultivation limit of Vitex rotundifloia, Tetragonia tetragonoides and Glehnia littoralis at coastal area and physiological vitality of RAW 264.7 cell and HL-60 cell. Korean J Med Crop Sci 16(1):44-50
  13. Kim SK, Kim IK, Lee GJ (2011) Growth responses of New Zealand spinach [Tetragonia tetragonioides (Pall.) Kuntze] to different soil texture and salinity. CNU J Agric Sci 38:631-639
  14. Kim IK, Lee KY, Kim SK, Kim BW, Choi WY, Lee GJ (2012) Preliminary screening of leafy vegetable New Zealand spinaches (Tetragonia tetragonioides) native to Korea. CNU J Agric Sci 39:515-523
  15. Kumar S, Tamura K, Dudley J, Nei M (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-1599
  16. Kurukulasuriya P, Rosenthal S (2003) Climate change and agriculture. A review of impacts and adaptations. Climate change series No.91. The World Bank Environment Department
  17. Muse SV, Liu KJ (2005) Powermarker: An integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128-2129
  18. Myeong HH, Lee JS, Jeon JY, Song MS (2011) Study on creation method of green space for port ecosystem using the halophytes. Kor J Soc Coastal Ocean Engineers 23(1):50-56
  19. Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genet 89:583-590


Supported by : 충남대학교