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Proteomic analysis of dehydroascorbate reductase transgenic potato plants

Dehydroascorbate reductase 과발현 형질전환 감자 식물체의 단백질체 분석

  • Received : 2016.05.11
  • Accepted : 2016.06.14
  • Published : 2016.06.30

Abstract

Ascorbic acid (AsA) is a strong antioxidant/reducing agent that can be converted to dehydroascorbate (DHA) by oxidation in plants. DHA, a very short-lived chemical, is recycled to AsA by dehydroascorbate reductase (DHAR). Previously, DHAR cDNA was isolated from the hairy roots of the sesame plant, and DHAR-overexpressing transgenic potato plants were generated under the control of the CaMV35S promoter (CaMV35S::DHAR). An increase in transgene expression and ascorbate levels were observed in the transgenic plants. In the present study, proteomic analysis revealed that transgenic plants not only accumulated DHAR in their cells, but also induced several other antioxidant enzyme-related proteins during plant growth. These results suggest that DHAR is important for stress tolerance via induction of antioxidant proteins, and could improve stress tolerance in transgenic potato plants.

Keywords

Potato;Dehydroascorbate reductase;Proteomics;Antioxidant enzyme;Ascorbate

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Acknowledgement

Supported by : 경남과학기술대학교