DOI QR코드

DOI QR Code

Studies on Bioactive Substances and Antioxidant Activities of Marine Algae from Jeju Island

제주도 연안 서식 해조류에 대한 주요 활성성분 및 항산화효능 연구

Kim, Tae-Hee;Ko, Seok-Chun;Oh, Gun-Woo;Park, Hyeon-Ho;Lee, Dae-Sung;Yim, Mi-Jin;Lee, Jeong Min;Yoo, Jong Su;Kim, Chang-Soo;Choi, Il-Whan;Jung, Won-Kyo
김태희;고석천;오건우;박현호;이대성;임미진;이정민;유종수;김창수;최일환;정원교

  • Received : 2016.05.31
  • Accepted : 2016.06.24
  • Published : 2016.06.30

Abstract

The objective of this study was to investigate the useful components and potential antioxidant activities of 30 marine algae, collected from Jeju Island in Korea. The contents of bioacitve substance of extracts from marine algae, such total polyphenol and carbohydrate, were determined. The extract of Distromium decumbens had the highest amount of total polyphenol content (21.27%), and that of Gracilaria incurvata Okamura had the highest amount of total carbohydrate content (10.18%). The antioxidant activities of extracts obtained from algae were tested through the evaluation of DPPH radical and hydroxyl radical scavenging activity. The extracts of Distromium decumbens, Sargassum hemiphyllum (Turner) C.Agardh, Sargassum serratifolium (C. Agardh) C. Agardh and Acrosorium yendoi Yamada were found to have more than 80% DPPH radical scavenging activity and that of Dictyota okamurae (Dawson) $H{\ddot{o}}tning$, Schnetter, et Prud'homme van Reine, Myagropsis myagroides (Martens ex Turner) Fensholt, Sargassum serratifolium (C. Agardh) C. Agardh and Cladophora wrightiana Harvey showed more than 50% hydroxyl radical scavenging activity. These results suggest that algae collected from Jeju Island would be good raw materials for antioxidant.

Keywords

Algae;Antioxidant;Free radical scavenging

References

  1. Kim, B. M., Jun, J. Y., Park, Y. B. and Jeong, I. H. 2006. Antioxidative activity of methanolic extracts from seaweeds. J. Korean Soc. Food Sci. Nur. 35, 1097-1101. https://doi.org/10.3746/jkfn.2006.35.8.1097
  2. Yoon, J. A., Yu, K, W., Jun, W. J., Cho, H. Y., Son, Y. S. and Yang, H. C. 2000. Screening of anticoagulant activity in the extracts of edible seaweeds and optimization of extraction condition. J. Korean Soc. Food Sci. Nutr. 29, 1098-1106.
  3. Vinoth Kumar, R., Murugesan, S., Bhuvaneshwari, S. and Thennarasan, S. 2016. In vitro antibacterial effecs of red alga Champia parvula (C. Agardh) of various solvents against human pathogenic bacterial. Int. J. Pharm. 4, 111-116.
  4. Choi, J. H., Kim, D. I., Park, S. H., Kim, D. W. and Koo, J. G. 1999. Effects of Sea Tangle (Laminaria japonica) and Fucoidan Components on the Attack of Oxygen Rad icals in Kidney. J. Korean Fish. Soc. 32, 758-763.
  5. Kim, M. J., Choi, J. S., Song, E. J., Lee, S. Y., Kim, K. B. W. R., Lee, S. J., Kim, S. J., Yoon, S. Y., Jeon, Y. J. and Ahn, D. H. 2009. Effects of heat and pH treatments on antioxidant properties of Ishige okamurai extract. Korean J. Food Sci. Technol. 41, 50-56.
  6. Lee, S. H., Park, M. H., Heo, S. J., Kang, S. M., Ko, S. C., Han, J. S. and Jeon. Y. J. 2010. Dieckol isolated from Ecklonia cava inhibits ${\alpha}$-glucosidase and ${\alpha}$-amylase in vitro and alleviates postprandial hyperglycemia in streptozotocin-induced diabetic mice. Food. Chem. Toxicol. 48, 2633-2637. https://doi.org/10.1016/j.fct.2010.06.032
  7. Kang, M. C., Cha, S. H., Wijesinghe, W. A. J. P., Kang, S. M., Lee, S. H., Kim, E. A., Song, C. B. and Jeon, Y. J. 2013. Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo. Food Chem. 48, 950-955.
  8. Lee, B. B., Park, S. R., Han, C. S., Han, D. Y., Park, E., Park, H. R. and Lee, S. C. 2008. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Viola mandshurica extracts. J. Korean. Soc. Food Sci. Nutr. 37, 405-409. https://doi.org/10.3746/jkfn.2008.37.4.405
  9. Lee, M. H., Lee, J. W., Park, C., Han, M. H., Hong, S. H. and Choi, Y. H. 2015. Antioxidant, Antimicrobial and A nticancer Properties of Seven Traditional Herb-combined Remedies. J. Life Sci. 25, 406-415. https://doi.org/10.5352/JLS.2015.25.4.406
  10. Hyon, J. S., Kang, S. M., Han, S. W., Kang, M. C., Oh, M. C., Oh, C. K., Kim, D. W., Jeon, Y. J. and Kim, S. H. 2009. Flavonoid component changes and antioxidant activities of fermented Citrus grandis Osbeck peel. J. Korean Soc. Nutr. 38, 1310-1316. https://doi.org/10.3746/jkfn.2009.38.10.1310
  11. Lee, K. M., Jeong, G. T. and Park, D. H. 2004. Study of antimicrobial and DPPH scavenger activity of wood vinegar. Korean J. Boptechnol. Bioeng. 19, 381-384.
  12. Hyun, J. M., Park, K. J., Kim, S. S., Park, S. M., Lee, Y. J. and An, H. J. 2015. Antioxidant and Anti-inflammatory Effects of Solvent Fractions from the Peel of the Native Jeju Citrus 'Hongkyool'and 'Pyunkyool. J. Life Sci. 25, 1132-1138. https://doi.org/10.5352/JLS.2015.25.10.1132
  13. Kim, S. J., Lee, G. S., Moh, S. H., Park, J., Auh, C. K., Chung, Y., Ryu, T. K. and Lee, T. K. 2013. Phenolic Contents and Antioxidant Activities of Six Edible Seawe eds. J. Korea Acad. Indistr. Coop. Soc. 14, 3081-3088.
  14. Ko, Y. H., Moon, Y. S., Sohn, S. H., Jung, C. Y. and Jang, I. 2012. Effect of Dietary Supplementation of Plum or Omija on Growth Performance, Blood Biochemical Profiles and Antioxidant Defense System in Broiler Chickens. Korean J. Poult. Sci. 39, 121-131. https://doi.org/10.5536/KJPS.2012.39.2.121
  15. Wijesinghe, W. A. J. P., Lee, W. W., Kim, Y. M., Kim, Y. T., Kim, S. K., Jeon, B. T., Kim, J. S., Heu, M. S., Jung, W. K., Ahn, G., Lee, K. W. and Jeon, Y. J. 2011. Value-added fermentation of Ecklonia cava processing by-product and its antioxidant effect. J. Appl. Phycol. 24, 201-209.
  16. Folin, O. and Denis, W. 1912. On phosphotungastic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12, 239-243.
  17. Duboi, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350-356. https://doi.org/10.1021/ac60111a017
  18. Nanjo, H., Adachi, H., Aketa, M., Mizoguchi, T., Nishihara, T. and Terada, T. 1995. The role of cysteine in the alteration of bovine liver dihydrodiol dehydrogenase 3 activity. Biochem. J. 17, 233-238.
  19. Rosen, G. M. and Rauckman, E. J. 1980. Spin trapping of the primary radical involved in the activation of the carcinogen N-hydroxy-2-acetylaminofluorene by cumene hydroperoxide-hematin. Mol. Pharmacol. 17, 233-238.
  20. Bae, H. M., Cho, E. K., Kim, H. Y., Kand, S. H. and Choi, Y. J. 2012. Biological Analysis of Enzymatic Extracts from Capsosiphon Fulvescens Using the Microbulbifer sp. AJ-3 Marine Bacterium. J. Life Sci. 22, 627-633. https://doi.org/10.5352/JLS.2012.22.5.627
  21. Kim, S. J., Lee, G., Moh, S. H., Park, J., Auh, C. H., Chung, Y., Ryu, T. K. and Lee, T. K. 2013. Phenolic contents and antioxidant activities of six edible seaweeds. J. Korea Acad. Industr. Coop. Soc. 14, 3081-3088. https://doi.org/10.5762/KAIS.2013.14.6.3081
  22. Kim, E. J., Choi, J. Y., Yu, M., Kim, M. Y., Lee, S. and Lee, B. H. 2012. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Korean J. Food Sci. Technol. 44, 337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  23. Na, H. S., Kim, J. Y., Park, J. S., Choi, G. C., Yang, S. I., Lee, J. H., Cho, J. Y. and Ma, S. J. 2014. Characteristics of marine algae extracts using subcritical water extract method. Korean. J. Food Preserv. 21, 62-68. https://doi.org/10.11002/kjfp.2014.21.1.62
  24. Lee, W. W., Ahn, G. A., Kang, N., Kim, E. A., Jee, Y. and Jeon, Y. J. 2015. Radio-protective Effect of a Polysaccharide from Ishige okamurae against Gamma Ray-irradiated Mouse Immune Cells. J. Chitin Chitosan 20, 229-236. https://doi.org/10.17642/jcc.20.4.2
  25. Kim, J. H., Kim, Y. H., Kim, S. K., Kim, B. W. and Nam, S. W. 2011. Properties and industrial applications of seaweed polysaccharides-degrading enzymes from the marine microorganisms. Korean J. Microbiolo. Biotechnology 39, 189-199.
  26. Choi, S. Y., Kim, S. Y., Hur, J. M., Choi, H. G. and Sung, N. J. 2006. Antioxidant Activity of Solvent Extracts from Sargassum thunbergii. J. Korean. Soc. Food Sci. Nutr. 35, 139-144. https://doi.org/10.3746/jkfn.2006.35.2.139
  27. Joung, Y. M., Park, S. J., Lee, K. Y., Suh, J. K., Hwang, S. Y., Park, K. E. and Kang, M. H. 2007. Antioxidative and antimicrobial activities of Lilium Species extracts prepared from different aerial parts. J. Korean. Soc. Food Sci. Nutr. 41, 156-160.
  28. Heo, S J., Cha, S. H., Lee, K. W., Cho, S. and Jeon, Y. J. 2005. Antioxidant activities of chlorophyta and phaeophyta from Jeju Island. Jour. Fish. Mar. Sci. Edu. 20, 251.
  29. Shin, J. H., Choi, D. J., Lee, S. J., Cha, J. Y. and Sung, N. J. 2008. Antioxidant Activity of Black Garlic (Allium sativum L.). J. Korean Soc. Food Sci. Nutr. 37, 965-971. https://doi.org/10.3746/jkfn.2008.37.8.965
  30. Pereira, L. 2011. A review of the nutrient composition of selected edible seaweeds. In: Seaweed and Vitor H. Pomin(eds), Seaweed: Ecology, nutrient composition and medicinal uses, Nova Science Publishers, pp 15-47.
  31. Kim, K. N., Heo, S. J., Cha, S. H. and Jeon, Y. J. 2006. Evaluation of DPPH radical scavenging activity of Jeju seaweeds using high throughput screening (HTS) technique. J. Mar. Biosci. Biotechnol. 1,170-177.

Acknowledgement

Supported by : 해양수산부, 한국연구재단, 국립해양생물자원관