Antioxidant and Anti-Melanogenic Activities of Hyssopus officinalis Extracts

히솝 추출물의 항산화 효과 및 멜라닌 생성 저해효과

  • Received : 2016.04.26
  • Accepted : 2016.06.24
  • Published : 2016.06.30


Hyssopus officinalis is a herbaceous plant of the genus Hyssopus. Due to its properties as an antiseptic, cough reliever and expectorant, it is commonly used as an aromatic herb and medicinal plant. This study was performed to investigate the anti-oxidative and anti-melanogenic properties of Hyssopus officinalis extracts (HE) using in vitro assays and cell culture systems. As a result, HE showed higher DPPH and ABTS radicals scavenging activity in a dose-dependent manner. Also, HE inhibited the prodution of intracellular ROS and melanin contents in B16F10 melanoma cell as well as tyrosinase activity. We also found that HE inhibit mRNA expression of MITF, tyrosinase and TRP-2 gene. These findings suggest that HE may be beneficial for preventing oxidative damage and melanogenesis of skin.


antioxidant;anti-melanogenesis;B16F10;Hysspus officinalis;tyrosinase


  1. C. A. Ferguson and S. H. Kidson, The regulation of tyrosinase gene transcription, Pigment Cell Res., 10(3), 127 (1997).
  2. D. Tobin, A. Quinn, S. Ito, and A. Thody, The presence of tyrosinase and related protein in human epidermis and their relationship in melanin type, Pigment Cell Res., 7(4), 204 (1994).
  3. K. N. Kim, H. M. Yang, S. M. Kang, D. Kim, C. Ahn, and Y. J. Jeon, Octaphlorethol a isolated from Ishige foliacea inhibits ${\alpha}$-MSH-stimulated induced melanogenesis via ERK pathway in B16F10 melanoma cells, Food Chem. Toxicol., 59, 521 (2013).
  4. S. Y. Choi, Y. C. Kim, and B. S. Chang, Inhibitory effect of black tea water extract on melanogenesis in melan-a cells and its action mechanism, Korean J. Microsc., 41(3), 169 (2011).
  5. H. C. Huang, W. Y. Hsieh, Y. L. Nin, and T. M. Chang, Inhibitory effects of adlay extract on melanin production and cellular oxygen stress in B16F10 melanoma cells, Int. J. Mol. Sci., 15(9), 16665 (2014).
  6. M. Tsatmali, J. Ancans, and A. J. Thody, Melanocyte fuction and its control by melanocortin peptides, J. Histochem. Cytochem., 50(2), 125 (2002).
  7. S. Alaluf, A. Heath, N. Carter, D. Atkins, H. Mahalingam, K. Barrett, R. Kolb, and N. smit, Variation in melanin content and composition in type V and VI photoexposed and photoprotected human skin: the dominant role of DHI, Pigment Cell Res., 14(5), 337 (2001).
  8. J. Cabanes, S. Chazarra, and F. Garcia-Carmona, Kojic acid, a cosmetic skin whitening agent, is aslow-binding inhibitor of catecholase activity of tyrosinase, J. Pharm. Pharmacol., 46(12), 982 (1997).
  9. V. Del Marmol and F. Beermann, Tyrosinase and related proteins in mammalian pigmentation, FEBS Lett., 381(3), 165 (1996).
  10. T. Kobayashi, K. Urabe, and A. J. Winder, Tyrosinase related protein (TRP-1) functions as a DHICA oxidase activity in melanin biosynthesis, EMBO J., 13(24), 5818 (1994).
  11. E. V. Curto, C. Kwong, H. Hermersdorfer, H. Glatt, C. Santis, V. Virador, V. J. Hearing, and P. Dooley, Inhibitors of mamalian melanocytes tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors, Biochem. Pharmacol., 57(6), 663 (1999).
  12. J. K. No, D. Y. Soung, Y. J. Kim, K. H. Shim, Y. S. Jun, S. H. Rhee, T. Yokizawa, and H. Y. Chung, Inhibition of tyrosinase by green tea components, Life Sci., 65(21), 241 (1999).
  13. G. Prota, Recent advances in the chemistry of melanogenesis in mammals, J. Invest. Dermatol., 75(122), 31 (1990).
  14. S. H. Lee, J. S. Park, S. Y. Kim, J. J. Kim, and S. R. Chung, Isolation of inhibitory components on tyrosinase activity from the bark of Paeonia moutan, J. Pharm. Soc. Korea, 42(4), 353 (1998).
  15. E. J. Seo, E. S. Hong, M. H. Choi, K. S. Kim, and S. J. Lee, Antioxidant and skin whitening effects of Rhamnus yoshinoi extracts, Korean J. Food Sci. Technol., 42(6), 750 (2010).
  16. J. Lopez, R. Tudela, F. Varon, G. Carmona, and F. G. Canovas, Analysis of a kinetic model for melanin biosynthesis pathway, J. Biol. Chem., 267(6), 381 (1992).
  17. R. Busca and R. Ballotti, Cyclic AMP a key messenger in the regulation of skin pigmentation, Pigment Cells Res., 13(2), 60 (2000).
  18. B. Saha, S. K. Singh, C. Sarkar, R. Bera, J. Ratha, D. J. Tobin, and R. Bhadra, Activation of the MITF promoter by lipid-stimulated activation of p38-stress signalling to CREB, Pigment Cells Res., 19(6), 595 (2006).
  19. I. Aksan and C. R. Goding, Targeting the microphthalmia basic helix-loop-helix-leucine zipper transcription factor to a subset of E-box elements in vitro and in vivo, Mol. Cell. Biol., 18(12), 6930 (1998).
  20. C. A. Hodgkinson, K. J. Moore, A. Nakayama, E. Steingrimsson, N. G. Copel, N. A. Jenkins, and H. Arnheiter, Mutations at the mouse microphthalmi a locus are associated with defects in a gene encoding a novel basic-helix-loop-helix-zipper protein, Cell, 74(2), 395 (1993).
  21. E. Steingrimsson, K. J. Moore, M. L. Lamoreux, A. R. Ferre-D'Amare, S. K. Burley, D. C. Zimring, L. C. Skow, C. A. Hodgkinson, H. Arnheiter, N. G. Copeland, and N. A. Jenkins, Molecular basis of mouse microphthalmia (mi) mutations helps explain their developmental and phenotypic consequences, Nat. Genet., 8(3), 256 (1994).
  22. H. R. Widlund and D. E. Fisher, Microphthalamia associated transcription factor: a critical regulator of pigment cell development and survival, Oncogene, 22(20), 3035 (2003).
  23. S. Mallick, S. K. Singh, C. Sarkar, B. Saha, and R. Bhadra, Human placental lipid induces melanogenesis by increasing the expression of tyrosinase and its related protein in vitro, Pigment Cell Res., 18(1), 25 (2005).
  24. J. P. Ortonne and T. Passeron, Melanin pigmentary disorders: treatment update, Dermatol. Clin., 23(2), 209 (2005).
  25. M. Nakagawa and K. Kawai, Contact allergy to kojic acid in skin care products, Contact Derm., 32(1), 9 (1995).
  26. E. K. Kwon, Y. E. Kim, C. H. Lee, and H. Y. Kim, Screening of nine herbs with biological activities on ACE inhibition HMG-CoA reductase inhibition, and fibrinolysis, Korean J. Food Sci. Technol., 38(5), 691 (2006).


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