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Effects of Seungseup-tang on MIA-induced Osteoarthritis Rats

승습탕(勝濕湯)이 MIA로 유발된 골관절염 흰쥐에 미치는 영향

  • Lee, Jong-Hoon (Department of Rehabilitaion Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Woo, Chang-Hoon (Department of Rehabilitaion Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Young-Jun (Department of Rehabilitaion Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Ahn, Hee-Duk (Department of Rehabilitaion Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University)
  • 이종훈 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 김영준 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 안희덕 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Received : 2016.06.16
  • Accepted : 2016.07.08
  • Published : 2016.07.31

Abstract

Objectives This study was designed to evaluate the anti-inflammatory effects of Seungseup-tang (SST) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods Osteoarthritis was induced by injection of MIA ($50{\mu}l$ with 80 mg/ml) into knee joint cavity of rats. Rats were divided into 4 groups (normal group, control group, indomethacin treated group, SST treated group, each n=6). Normal group was not injected with MIA and taken normal diet. Control group was injected with MIA and taken with distilled water. Indomethacin treated group was injected with MIA and taken indomethacin 5 mg/kg by oral administration. SST treated group was injected with MIA and taken SST 200 mg/kg by oral administration. We examined the weight-bearing ability of hind paw, biomarkers related to oxidative stress in serum, inflammatory proteins and inflammatory mediators and cytokines. Moreover, histopathological examination of knee joint structure was also performed by Hematoxylin & Eosin (H&E), Safranin-O staining method. Results In the present study, SST treated group showed a similar inhibitory effects alike indomethacin treated group, in most of the studied parameters of inflammation. The increased oxidative stress biomarker such as reactive oxygen species (ROS) and peroxy nitrite ($ONOO^-$) in the serum were reduced with SST. Especially, the level of $ONOO^-$ compared with control group significantly suppressed. Also, the expression of inflammatory mediators and cytokines induced by nuclear factor-kappa B (NF-${\kappa}B$) activation was modulated through inhibition of IkBa phosphorlation. In addition, histological analysis revealed that cartilage damage by MIA repaired markedly in SST treated group. Conclusions According to the results, Seungseup-tang may be effective for preventing the progression of osteoarthritis.

References

  1. The Society of Korean Medicine Rehabilitation. Korean Medicine Rehabilitation 3rd edition. Seoul:Koonja-publishers. 2012:84-98.
  2. The Korean Orthopaedic Association. Orthopedics 7th edition. Seoul:Choesinuihaksa. 2013:317-27.
  3. Heo J. Treasured Mirror of Eastern Medicine. Seoul: Bubin-publishers. 2007:1159.
  4. Kim SH, Park YK. Effects of Atractylodis Rhizoma Alba extract on collagen-induced arthritis in mice. Kor. J. Herbology. 2012;27(3):1-6. https://doi.org/10.6116/KJH.2012.27.3.1
  5. Park EK, Shin YW, Lee HU, Kim SS, Lee YC, Lee BY, et al. Inhibitory effect of ginsenoside Rb1 and compound K on NO and prostaglandin E2 biosynthesis of RAW 264.7 cells induced bylipopolysaccharide. Biol Pharm Bull. 2005;28:652-6. https://doi.org/10.1248/bpb.28.652
  6. Jung TY. Inhibitory effect of Korean ginseng extract on type II collagen-induced arthritis. Seoul:Graduate school, Yonsei Univ. 2009.
  7. Ryu HS, Kim HS. Effect of Zingiber officinale roscoe extracts on mice immune cell activation. The Korean Journal of Nutrition Korean. 2004;37(1):23-30.
  8. Kim BW. Anti-inflammatory effect of Jakyakgamcho-tang. Korean J. Orient.Int. Med. 2010;31(2):365-71.
  9. Kim YH, Yim YK, Lee H. The effects of herbal-acupuncture with Aconiti Iateralis Preparata Radix solution on collagen-induced arthritis. 2006;23(2):137-54.
  10. Kim SJ, Park SM, Kang H, Shim BS, Kim SH, Choi SH, Ahn KS. Anti-inflammatory Effects of Cinnamomi Ramulus on Collagen Induced Arthritis - a Model for Rheumatoid Arthritis in DBA/1J Mice and Cytokine Production in Raw264.7 Cells. Korean J. Oriental Physiology & Pathology. 2008;22(3):542-7.
  11. Rios J.L. Chemical constituents and pharmacological properties of Poria cocos. PlantaMed. 2011;77(7):681-91.
  12. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med. 1994;16(2):149-56. https://doi.org/10.1016/0891-5849(94)90138-4
  13. Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978;86(1):271-8. https://doi.org/10.1016/0003-2697(78)90342-1
  14. Seidel MF, Herguijuela M, Forkert R & Otten U. Nerve growth factor in rheumatic diseases. Semin Arthritis Rheum. 2010;40(2):109-26. https://doi.org/10.1016/j.semarthrit.2009.03.002
  15. Bondeson J, Blom AB, Wainwright S, Hughes C, Caterson B, van den Berg WB. The role of synovial macrophages and macrophage-produced mediators in driving inflammatory and destructive responses in osteoarthritis. Arthritis Rheum. 2010;62(3):647-57. https://doi.org/10.1002/art.27290
  16. Frisch SM, Screaton RA. Anoikis mechanisms. Curr Opin Cell Biol. 2001;13(5):555-62. https://doi.org/10.1016/S0955-0674(00)00251-9
  17. Yun JH. Osteoarthritis update. The Korean Journal of Medicine. 2012;82(2):170-4. https://doi.org/10.3904/kjm.2012.82.2.170
  18. Goldring MB, Otero M, Plumb DA, Draqomir C, Favero M, El Hachem K, et al. Roles of inflammatory and anabolic cytokines in cartilage metabolism:signals and multiple effectors converge upon MMP-13 regulation in osteoarthritis. Eur Cell Mater 2011;21:202-20. https://doi.org/10.22203/eCM.v021a16
  19. Yoh SB, Sul JU, Shin MS. Research trends on the treatment of knee Osteoarthritis in Korean medicine. The Korean Journal of Acupuncture. 2011;28(1):139-55.
  20. Hwang DY. Compilation of Formulas and Medicinals. Seoul:Yeongnimsa. 2010:130-1.
  21. National College of Korean Medicine. Herbal medicine. Seoul:Yeongnimsa. 2004:156-7, 348-50, 375-7, 378-80, 575-8, 581-2, 586-8, 637-8.
  22. Kalbhen DA, Blum U. Theoretisches Koncept and experimentelle bestatigung fur ein neues arthos modell amversuchstier. Arzneimittelforschung. 1977;27(3):527-31.
  23. Combe R, Bramwell S, Field MJ. The monosodium iodoacetate model of osteoarthritis: a model of chronic nociceptive pain in rats? Neurosci Lett. 2004;370(2-3):236-40. https://doi.org/10.1016/j.neulet.2004.08.023
  24. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicol Pathol. 2003;31(6):619-24. https://doi.org/10.1080/01926230390241800
  25. Zhu CZ, Hsieh G, Ei-Kouhen O, Wilson SG, Mikusa JP, Hollingsworth PR, Chang R, Moreland RB, Brioni J, Decker MW, Honore P. Role of central and peripheral mGluR5 receptors in post-operative pain in rats. Pain. 2005;114(1-2):195-202. https://doi.org/10.1016/j.pain.2004.12.016
  26. McCord JM. The evolution of free radicals and oxidative stress. Am J Med. 2000;108(8):652-9. https://doi.org/10.1016/S0002-9343(00)00412-5
  27. Radi R, Beckman JS, Bush KM and Freeman BA. 1991. Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Archives of Biochemistry and Biophysics. 1991;288(2):481-7. https://doi.org/10.1016/0003-9861(91)90224-7
  28. Cho YS, Choi JH, Zhang SY, Lee JH, Hwang KK, Kim HS, Oh BH, Lee MM, Park YB, Choi YS. Relationship of Polymorphisms in the Oxidative Stress Related Genes - Paraoxonase and $p22^{phox}$ - to Variant Angina and Coronary Artery Stenosis in Korean. Korean Circulation J. 2003;33(2):104-112. https://doi.org/10.4070/kcj.2003.33.2.104
  29. K. A. Radermacher, K. Wingler, P. Kleikers, S. Altenhofer, J. J. Hermans, C. Kleinschnitz, S. H. Hhw, The 1027th target candidate in stroke: Will NADPH oxidase hold up?. Exp Transl Stroke Med. 2012;4(1):1-11. https://doi.org/10.1186/2040-7378-4-1
  30. Park SH, Lee HJ, Ryu JH, Lee SY, Shin HD, Hong JH, Seok JH, Lee CJ. Effects of Silibinin and Resveratrol on Degradation of $I{\kappa}B$ and Translocation of NF-${\kappa}B$ p65 Induced by Tumor Necrosis Factor-${\alpha}$ in Cultured Airway Epithelial Cells. J Pharm. 2014;58(1):1-6.
  31. Asagiri M, Takayanagi H. The molecular understanding of osteoclast differentiation. Bone. 2007;40(2):251-264. https://doi.org/10.1016/j.bone.2006.09.023
  32. Mastbergen SC, Marijnissen AC, Vianen ME, Zoer B, van Roermund PM, Bijlsma JW, Lafeber FP. Inhibition of COX-2 by celecoxib in the canine groove model of osteoarthritis. Rheumatology. 2006;45(4):405-13. https://doi.org/10.1093/rheumatology/kei187
  33. Abramson SB. Osteoarthritis and nitric oxide. Osteoarthritis Cartilage. 2008;16:15-20.
  34. Sakaguchi Y, Shirahase H, Ichikawa A, Kanda M, Nozaki Y, Uehara Y. Effects of selective iNOS inhibition on type II collagen-induced arthritis in mice. Life Sci. 2004;75(19):2257-67. https://doi.org/10.1016/j.lfs.2004.02.037
  35. Brune B, Zhou J, von Knethen A. Nitric oxide, oxidative stress, and apoptosis. Kidney Int Suppl. 2003;(84):S22-4.
  36. Smolen JS, Aletaha D, Koeller M, Weisman MH, Emery P. New therapies for treatment of rheumatoid arthritis. Lancet. 2007;370(9602):1861-74. https://doi.org/10.1016/S0140-6736(07)60784-3
  37. Iking-Konert C, Bartz-Bazzanella P, Falagan D, Hofman MW, Schwarting A, Dorner T. Interleukin-6 inhibition as a potential therapeutic target in rheumatic diseases. Z Rheumatol. 2014;73(3):269-76. https://doi.org/10.1007/s00393-013-1268-9
  38. Yao X, Huang J, Zhong H, Shen N, Faggioni R, Fung M, Yao Y. Targeting interleukin-6 in inflammatory autoimmune diseases and cancers. Pharmacol Ther. 2014;141(2):125-39. https://doi.org/10.1016/j.pharmthera.2013.09.004
  39. Lee SH, Kwon KD, Lee SW, Cho SH, Ahn HS. Acetabular degeneration in osteonecrosis of the femoral head. J. of Korean Orthop.Assoc. 2004;39(3):239-46. https://doi.org/10.4055/jkoa.2004.39.3.239
  40. Kim NY, Song IS, Kim JR, Kim YS. Toxic effect of NSAID on IEC18 cells and its mechanism. Korean Journal of Medicine. 1993;45(6):759-69.
  41. Song YJ, Ha CW. The use of COX-2 selective nonsteroidal anti-inflammatory drugs for the treatent of osteoarthritis. J Korean Knee Soc. 2009;21(2):84-92.
  42. Cho YJ, Moon SL, Park KH, Cho NC, Song YW. Comparison of side effects of non-steroidal antiinflammatory drugs(NSAIDs) in rheumatoid arthritis patients. J. Kor. Soc. Hosp. Pharm. 1998;15(2):186-92.