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Analgesic Effects of ChondroT in Collagenase-induced Osteoarthritis Rat Model

Collagenase로 유발된 동물모델에서 ChondroT의 진통효과에 관한 연구

  • Won, Jeong-Yoon (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Dongshin University) ;
  • Jeong, Ji-Won (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Dongshin University) ;
  • Na, Chang-Su (Department of Meridian and Acupoint, College of Korean Medicine, Dongshin University) ;
  • Kim, Seon-Jong (Department of Korean Rehabilitation Medicine, College of Korean Medicine, Dongshin University)
  • 원정윤 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 정지원 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 나창수 (동신대학교 한의과대학 경락경혈학회) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학과교실)
  • Received : 2016.06.16
  • Accepted : 2016.07.02
  • Published : 2016.07.31

Abstract

Objectives The purpose of this study was to find out the effects of ChondroT on arthralgia of the Collagenase-induced osteoarthritis in rats. Methods Osteoarthritis was induced into rat by injecting Collagenase in its knee joint. Rats are divided into a total of 8 groups (n=6). Normal group was not induced for osteoarthritis whereas control groups were induced for osteoarthritis by Collagenase. Positive-A (Indomethacin) was injected with Collagenase and after 8 days, 2 mg/kg of Indomethacin was medicated. Positive-B (JOINS TAB) was injected with Collagenase and after 8 days, 20 mg/kg of JOINS TAB was medicated. Experimental groups (Chondro T) at three dose levels (50, 100 and 200 mg/kg) were injected with Collagenase and after 8days they were medicated with 10 ml/kg. Indomethacin, JOINS TAB and ChondroT were medicated each substances once a day for 10 days. Thereafter, the changes in plantar withdrawal response of osteoarthritis rats by dynamic plantar aesthesiometer were observed and then RT-PCR analysis was done to investigate the expression of related proteins. Results 1. ChondroT significantly decreased withdrawal response of mechanical allodynia compared with control group in all of the experimental groups (ChondroT-A, ChondroT-B, ChondroT-C). 2. ChondroT significantly reduced Bax/Bcl-2 ratio in all of the experimental groups (ChondroT-A, ChondroT-B, ChondroT-C). 3. ChondroT significantly reduced the expression of INF-${\gamma}$ compared with control group in group ChondroT-B, ChondroT-C. Conclusions This results suggest that ChondroT may be meaningful for suppressing the pain of osteoarthritis. Further study is needed to conduct a rigorous clinical research.

Acknowledgement

Supported by : 보건복지부

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