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Antioxidant and tyrosinase inhibitory activities of immature fruits of Malus pumila cv. Fuji

미성숙 사과의 항산화 및 tyrosinase 저해 활성 평가

  • Kwon, O Jun (Gyeongbuk Institute For Regional Program Evaluation)
  • 권오준 ((재)경북지역사업평가단 평가팀)
  • Received : 2016.06.27
  • Accepted : 2016.07.11
  • Published : 2016.08.30

Abstract

In this study, the antioxidant and tyrosinase inhibitory activities of aqueous ethanolic extract from the immature fruits of Malus pumila cv. Fuji were evaluated. The antioxidant capacities of the extract was investigated employing radical scavenging assays using DPPH and $ABTS^+$ radicals. The skin-whitening effect of M. pumila cv. Fuji extract was tested using mushroom tyrosinase assay. In addition, the total phenolic content was measured by a spectrophotometric analysis. All tested samples showed a dose-dependent radical scavenging and tyrosinase inhibitory activities. Among the tested samples, the ethyl acetate (EtOAc)-soluble portion from the immature fruits of M. pumila cv. Fuji was showed the significant DPPH and $ABTS^+$ radicals scavenging activities. Also, the tyrosinase inhibitory activity of the ethyl acetate (EtOAc)-soluble portion from immature apples was higher than other solvent-soluble portion. These results suggest that unripe fruits of M. pumila cv. Fuji could be considered as a new valuable source of natural antioxidant and skin-whitening agents. Systematic investigation of immature fruits of Malus pumila cv. Fuji will be performed for the further development of its biological properties.

References

  1. Videla LA, Fermandez V (1988) Biochemical aspects of cellular oxidative stress. Arch Biol Med Exp, 21, 85-92
  2. Halliwell B, Aruoma OJ (1991) DNA damage by oxygen-derived species. FEBS Lett, 281, 9-19 https://doi.org/10.1016/0014-5793(91)80347-6
  3. Jennings PE, Barnett AH (1988) New approaches to the pathogenesis and treatment of diabetic microangiopathy. Diabetic Med, 5, 111-117 https://doi.org/10.1111/j.1464-5491.1988.tb00955.x
  4. Shim JS, Kim SD, Kim TS, Kim KN (2005) Biological activities of flavonoid glycosides isolated from Angelica keiskei. Korean J Food Sci Technol 37, 78-83
  5. Farag RS, Badel AZMA, Hewedi FM, EL-baroty GSA (1989) Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. J Am Oil Chem Soc, 66 792-799
  6. Frei B (1994) National antioxidants in human health and disease. Academic Press, San Diego, CA, USA, p 44-55
  7. Branen AL (1975) Toxicology and biochemistry of butylated hydroxyanisole and bytylated hydroxytoluene. J AM Oil Chem Soc, 52, 59-63 https://doi.org/10.1007/BF02901825
  8. Masaki H, Sakaki S, Atsumi T, Sakurai H (1995) Active-oxygen scavenging activity of plant extracts. Biol Pharm Bull, 18, 162-166 https://doi.org/10.1248/bpb.18.162
  9. Prota G (1980) Recent advances in the chemistry of melanogenesis in mammals. J Invest Dermatol, 75, 122-127 https://doi.org/10.1111/1523-1747.ep12521344
  10. Pavel S, Muskiet FA (1983) Eumelanin (precursor) metabolites as markers for pigmented malignant melanoma, a preliminary report. Cancer Detect Prev, 6, 311-316
  11. Hearing VJ, Jimenez M (1987) Mammalian tyrosinase the critical regulatory control point in melanocyte pigmentation. Int J Biochem, 19, 1141-1147 https://doi.org/10.1016/0020-711X(87)90095-4
  12. Maeda K, Naitou T, Umishio K, Fukuhara T, Motoyama A (2007) A novel melanin inhibitor: hydroperoxy traxastane-type triterpene from flowers of Arnica montana. Biol Pharm Bull, 30, 873-879 https://doi.org/10.1248/bpb.30.873
  13. Badria FA, elGayyar MA (2001) A new type of tyrosinase inhibitors from natural products as potential treatments for hyperpigmentation. Boll Chim Farm, 140, 267-271
  14. Akiyama H, Sakushima J, Taniuchi S, Kanda T, Yanagida A, Kojima T, Teshima R, Kobayashi Y, Goda Y, Toyoda M (2000) Antiallergic effect of apple polyphenols on the allergic model mouse. Biol Pharm Bull, 23, 1370-1373 https://doi.org/10.1248/bpb.23.1370
  15. Tokura T, Nakano N, Ito T, Matsuda H, Nagasako- Akazome Y, Kanda T, Ikeda M, Okumura K, Ogawa H, Nishiyama C (2005) Inhibitory effect of polyphenolenriched apple extracts on mast cell degranulation in vitro targeting the binding between IgE and FcepsilonRI. Biosci Biotechnol Biochem, 69, 1974-1977 https://doi.org/10.1271/bbb.69.1974
  16. Sugiyama H, Akazome Y, Shoji T, Yamaguchi A, Yasue M, Kanda T, Ohtake Y (2007) Oligomeric procyanidins in apple polyphenol are main active components for inhibition of pancreatic lipase and triglyceride absorption. J Agric Food Chem, 55, 4604-4609 https://doi.org/10.1021/jf070569k
  17. Yoshioka Y, Akiyama H, Nakano M, Shoji T, Kanda T, Ohtake Y, Takita T, Matsuda R, Maitani T (2008) Orally administered apple procyanidins protect against experimental inflammatory bowel disease in mice. Int Immunopharmacol, 8, 1802-1807 https://doi.org/10.1016/j.intimp.2008.08.021
  18. Corder R, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A (2006) Oenology: Red wine procyanidins and vascular health. Nature, 444, 566 https://doi.org/10.1038/444566a
  19. Blois MS (1958) Antioxidant activity determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  20. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidnt activity applying and improved ABTS radical cation decolorization assay. Free Radic Biol Med, 26, 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  21. Kwon OJ, Lee HY, Kim TH, Kim SG (2014) Antioxidant and pancreatic lipase inhibitory activities of Anemarrhena asphodeloides. Korean J Food Preserv, 21, 421-426 https://doi.org/10.11002/kjfp.2014.21.3.421
  22. Kwon OJ, Bae JS, Lee HY, Hwang JY, Lee EW, Ito H, Kim TH (2013) Pancreatic lipase inhibitory gallotannins from Galla Rhois with inhibitory effects on adipocyte differentiation in 3T3-L1 cells. Molecules, 18, 10629-10638 https://doi.org/10.3390/molecules180910629
  23. Yagi A, Kanbara T, Morinobu N (1987) Inhibition of mushroom-tyrosinase by aloe extract. Planta Med, 53, 515-517 https://doi.org/10.1055/s-2006-962798
  24. Gao X, Bjor, L, Trajkovski V, Uggla M (2000) Evaluation of antioxidant activities of rosehip ethanol extracts in different test system. J Sci Food Agric, 80, 2021-2027 https://doi.org/10.1002/1097-0010(200011)80:14<2021::AID-JSFA745>3.0.CO;2-2
  25. Torel J, Cillard J, Cillard P (1986) Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry, 25, 383-385 https://doi.org/10.1016/S0031-9422(00)85485-0
  26. Wang SY, Chang HN, Lin KT, Lo CP, Yang NS, Shyur LF (2003) Antioxidant properties and phytochemical characteristics of extracts from Lactuca indica. J Agric Food Chem, 51, 1506-1512 https://doi.org/10.1021/jf0259415
  27. Chai WM, Wei MK, Deng RG, Zou ZR, Peng YY (2015) Avocado proanthocyanidins as a source of tyrosinase inhibitors: structure characterization, inhibitory activity, and mechanism. J Agric Food Chem, 63, 7381-7387 https://doi.org/10.1021/acs.jafc.5b03099
  28. Lall N, Kishore N, Momtaz S, Hussein A, Naidoo S, Nqephe M, Crampton B (2015) Extract from Ceratonia siliqua exhibits depigmentation properties. Phytother Res, 29, 1729-1736 https://doi.org/10.1002/ptr.5420
  29. Park EK, Ahn SR, Kim DH, Lee EW, Kwon HJ, Kim BW, Kim TH (2014) Effects of unripe apple polyphenols on the expression of matrix metalloproteinase-1 and type-1 procollagen in ultraviolet irradiated human skin fibroblasts. J Korean Soc Appl Biol Chem, 57, 449-455 https://doi.org/10.1007/s13765-014-4128-7
  30. Olszewska MA, Michel P (2012) Activity-guided isolation and identification of free radical-scavenging components from various leaf extracts of Sorbus aria (L.) Crantz. Nat Prod Res, 26, 243-254 https://doi.org/10.1080/14786419.2010.537271