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Screening of yeast for alcoholic fermentation of no sugar-added tomato concentrate

토마토 농축액을 이용한 무가당 알코올 발효를 위한 효모의 선발

  • Kim, Dong-Hwan (Department of Food and Food Science and Technology, Kyungpook National University) ;
  • Moon, Jae-Nam (School of Food Science and Biotechnology, Korea Food Research Institute, Kyungpook National University) ;
  • Lee, Seul (School of Food Science and Biotechnology, Korea Food Research Institute, Kyungpook National University) ;
  • Lee, Soo-Won (Department of Food and Food Science and Technology, Kyungpook National University) ;
  • Moon, Hey-Kyung (Center for Scientific Instruments, Kyungpook National University) ;
  • Kim, Gwi-Young (Department of Food and Food Science and Technology, Kyungpook National University)
  • 김동환 (경북대학교 식품외식산업학과) ;
  • 문재남 (경북대학교 식품공학부) ;
  • 이슬 (경북대학교 식품공학부) ;
  • 이수원 (경북대학교 식품외식산업학과) ;
  • 문혜경 (경북대학교 공동실험실습관) ;
  • 김귀영 (경북대학교 식품외식산업학과)
  • Received : 2016.05.31
  • Accepted : 2016.07.05
  • Published : 2016.08.30

Abstract

The purpose of this study was to screen desirable yeast strains for alcoholic fermentation of tomato paste without sugar addition. The moisture, crude protein, crude fat, ash, and soluble nitrogen contents of the tomato paste ($25^{\circ}Brix$) were found to be 67.33%, 1.90%, 0.03%, 0.02%, and 30.72%, respectively. Free sugars found in the paste were fructose and glucose. Most abundant free amino acids of the paste were glutamic, aspartic, and ${\gamma}$-aminobutyric acids. Total seven yeast strains (Saccharomyces cerevisiae KDH (TWA), S. cerevisiae Lalvin ICVD-47 (TWB), S. cerevisiae Lalvin RC-212 (TWC), S. cerevisiae Lalvin K1-V1116 (TWD), S. bayanus Lalvin EC-1118 (TWE), S. cerevisiae Enoferm (TWF), and S. cerevisiae DJ97(KCTC8842P) (TWG)) were tasted for alcohol fermentation of the tomato paste. The highest alcohol content (8.2%) and the lowest residual sugar content ($13.25^{\circ}Brix$) were observed in the tomato paste fermented using the S. cerevisiae Lalvin ICVD-47 strain (TWB) after 3 day and 4 day of fermentation, respectively. Sugar and reducing sugar contents, and pH of the tomato paste were not remarkably affected by the difference in yeast strains used, showing $13.25{\sim}13.45^{\circ}Brix$, 28.37~28.48 mg/mL, and 4.43~4.54, respectively, after 4 day of fermentation. Color and total acid content were significantly affected by the types of yeast strains and fermentation time, but the numerical changes were negligible. These results indicate that TWB would be the suitable strain for alcoholic fermentatiom of tomato paste based on its highest alcohol production and the lowest residual sugar content produced during fermentation.

Acknowledgement

Supported by : 경북대학교

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