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Investigation of Microbial Contamination Level during Production of Baby Leafy Vegetables

어린잎채소 생산 농장의 위생지표세균과 병원성미생물 오염도 조사

  • Lee, Eun-Sun (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAS, RDA) ;
  • Kwak, Min-Gyu (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAS, RDA) ;
  • Kim, Won-Il (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAS, RDA) ;
  • An, Hyun Mi (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAS, RDA) ;
  • Lee, Hyo-Sup (Chemical Safety Division, Agro-Food Safety and Crop Protection Department, NAS, RDA) ;
  • Ryu, Song-Hee (Chemical Safety Division, Agro-Food Safety and Crop Protection Department, NAS, RDA) ;
  • Kim, Hwang-Yong (Division for Korea Program on International Agriculture, Technology Cooperation Bureau, RDA) ;
  • Ryu, Jae-Gee (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAS, RDA) ;
  • Kim, Se-Ri (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, NAS, RDA)
  • 이은선 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 곽민규 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김원일 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 안현미 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 이효섭 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 류송희 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 김황용 (농촌진흥청 국외농업기술과) ;
  • 류재기 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김세리 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀)
  • Received : 2016.06.28
  • Accepted : 2016.08.10
  • Published : 2016.08.30

Abstract

The purpose of this study was to investigate contamination sources of baby leafy vegetables by assessing microbial loads on baby leafy vegetables and agricultural inputs contacted with the vegetables. To estimate microbial loads, fecal indicators (coliform and Escherichia coli) and foodborne pathogens (E. coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus) were examined. A total of 126 samples including eleven kinds of leafy vegetables, irrigation water, media, and tools were tested, resulting in coliform contamination observed from most of samples. For E. coli, 10.3% (13/126) of the samples were positive including irrigation water, knife, handler, media, tools, and three kinds of leafy vegetables. B. cereus was detected from 38% (48/126) of the samples including media, tools and three kinds of leafy vegetables. No E. coli O157:H7, Salmonella spp., and L. monocytogenes was detected. This result implies that contacting with agricultural inputs could explain microbial load of baby leafy vegetables.

본 연구는 어린잎 채소와 생산환경에서 미생물학적 오염도를 조사하고자 수행하였다. 이를 위하여 11종의 어린잎채소와 종자, 관개용수, 상토, 작업도구 및 작업자 장갑 등 총 126개의 시료를 채취하여 위생지표세균(대장균군, E. coli)과 병원성미생물(Escherichia coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus)을 조사하였다. 그 결과, 종자와 관개용수를 제외한 대부분의 시료에서 대장균군이 검출되었다. E. coli는 분석시료 중 10.3% (13/126)에서 검출되었으며 검출된 시료로는 관개용수, 칼, 작업자 장갑, 및 다채, 적무, 청경채였다. 또한 B. cereus는 상토, 작업도구 및 청경채, 비트, 적근대 등 총 38% (48/126)의 시료에서 검출되었다. 한편 E. coli O157:H7, Salmonella spp., L. monocytogenes는 검출되지 않았다. 본연구의 결과로 미루어 볼 때 어린잎채소는 미생물 안전성면에서는 크게 우려할 수준은 아니지만 어린잎채소의 미생물오염을 사전 예방하는 차원에서 농업현장에서 쉽게 실천할 수 있는 위생관리 기술 개발과 보급이 필요하다고 판단된다.

Keywords

Acknowledgement

Supported by : 국립농업과학원

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