DOI QR코드

DOI QR Code

Comparative bioavailability of synthetic vitamin C and Nutra-C (calcium ascorbate) in Korean healthy volunteers

  • Choi, Kyung-Mi ;
  • Hoon, Kim Man ;
  • Won, Hwang Tae ;
  • Kim, Jong-Dae ;
  • Park, Keum duck ;
  • Kim, Mi-Young ;
  • Jung, Young-Rim ;
  • Shin, Hye-Seoung
  • Received : 2016.07.03
  • Accepted : 2016.08.24
  • Published : 2016.08.25

Abstract

The purpose of this study was to compare the relative bioavailability of synthetic Vitamin C and Nutra-C® (calcium ascorbate) using a randomized parallel pharmacokinetics study design. Under fasting conditions, 20 healthy volunteers were randomly allocated to receive a single oral dose (500 mg of ascorbic acid) of either synthetic Vitamin C or Nutra-C®. Fasting blood was collected pre-dose and 1, 2, 3, 4, 7 and 10 hr post-dose. The ascorbic acid content of human serum was determined using HPLC with ultraviolet detection. The fasting serum ascorbic acid concentrations of synthetic Vitamin C and Nutra-C® were 6.734 ± 2.09 ng/mL (n = 10) and 7.542 ± 2.96 ng/mL (n = 10), respectively. The bioavailability of Nutra-C® was significantly greater (128 %, p < 0.05) than that of the synthetic Vitamin C.

Keywords

Vitamin C;Nutra-C;Bioavailability;Human serum

References

  1. Y. Li and H. E. Schellhorn, Br. J. Nutr., 137, 2171-2184 (2007).
  2. A. C. Carr and B. Frei, Am. J. Clin. Nutr., 69, 1086-1107 (1999).
  3. A. R. Tricker and R. Preussmann, Mutat. Res., 259, 277-289 (1991). https://doi.org/10.1016/0165-1218(91)90123-4
  4. R. A. Scanlan, Nitrosamines and cancer. Linus Pauling Institute website. http://lpi.oregonstate.edu/f-w00/nitrosamine.html (2000).
  5. National Institutes of Health (NIH), Office of Dietary Supplements. Vitamin C. Dietary Supplement Fact Sheet. http://ods.od.nih.gov/factsheets/VitaminC-HealthProfessional/ (2013).
  6. K. Beck, C. A. Conlon, R. Kruger and J. Coad and W. Stonehouse, Br. J. Nutr., 105, 101-109 (2011). https://doi.org/10.1017/S0007114510003144
  7. L. Hallberg, M. Brune and L. Rossander, Hum. Nutr. Appl. Nutr., 40, 97-113 (1986).
  8. N. M. Scheers and A. S. Sandberg, Br. J. Nutr., 105, 1734-1740 (2011). https://doi.org/10.1017/S0007114510005672
  9. C. J. Bates, K. S. Jones and L. J. Bluck, Br. J. Nutr., 91, 699-705 (2004). https://doi.org/10.1079/BJN20041103
  10. A. R. Mangels, G. Block, C. M. Frey, B. H. Patterson, P. R. Taylor, E. P. Norkus and O. A. Levander, J. Nutr., 123, 1054-1061 (1993).
  11. F. R. Keltz, C. Kies and H. M. Fox, Am. J. Clin. Nutr., 31, 1167-1171 (1978).
  12. J. N. Hathcock, A. Azzi, J. Blumberg, T. Bray, A. Dickinson, B. Frei, I. Jialal, C. S. Johnston, F. J. Kelly and K. Kraemer, Am. J. Clin. Nutr., 81, 736-745 (2005).
  13. M. Levine, C. Conry-Cantilena, Y. Wang, R. W. Welch, P. W. Washko, K. R. Dhariwal, J. B. Park, A. Lazarev, J. F. Graumlich and J. King, Proc. Natl. Acad. Sci., 93, 3704-3709 (1996). https://doi.org/10.1073/pnas.93.8.3704
  14. S. Yung, M. Mayersohn and J. B. Robinson, J. Pharm. Sci., 71, 282-285 (1982). https://doi.org/10.1002/jps.2600710304
  15. H. N. Bhagavan and B. I. Wolkoff, Pharm. Res., 10, 239-242 (1993). https://doi.org/10.1023/A:1018938911420
  16. R. Sacharin, T. Taylor and L. F. Chasseaud, Int. J. Vitam. Nutr. Res., 47, 68-74 (1977).
  17. K. Nyyssonen, H. E. Poulsen, M. Hayn, P. Agerbo, E. Porkkala-Sarataho, J. Kaikkonen, R. Salonen and J. T. Salonen, Eur. J. Clin. Nutr., 51, 154-163 (1997). https://doi.org/10.1038/sj.ejcn.1600376
  18. M. J. Bush and A. J. Verlangieri, Res. Commun. Chem. Pathol. Pharmacol., 57, 137-140 (1987).
  19. A. J. Verlangieri, M. J. Fay and A. W. Bannon, Life Sci., 48, 2275-2281 (1991). https://doi.org/10.1016/0024-3205(91)90343-A
  20. C. S. Johnston and B. Luo, J. Am. Diet. Assoc., 94, 779-781 (1994). https://doi.org/10.1016/0002-8223(94)91950-X
  21. J. Gruenwald, H. J. Graubaum, R. Busch and C. Bentley, Adv. Ther., 23, 171-178 (2006). https://doi.org/10.1007/BF02850358
  22. M. S. Şerban, Gh. Câmpeanu and E. Ionescu, Metode de Laborator ín Biochimia Animală , Editura Didactica si Pedagogica, Bucuresti, Romania, 1993.
  23. C. Cofan and C. Radovan, Sensors, 8, 3952-3969 (2008). https://doi.org/10.3390/s8063952
  24. C. Papuc, A. Pop and M. Şerban, Metode Analitice in Biochimia Veterinara, Editura Printech, Bucuresti, Romania, 2001.
  25. D. Balan, M. Pele, M. Artimon and G. Luta, “Bioactive compounds in sea buckthorn fruits and in some products obtained by their processing”, Revue de Cytologie et de Biologie Végétales-Le Botaniste, 28, 364-368 (2005).
  26. N. Matei, V. Magearu, S. Birghilă , and S. Dobrinaş, Revista de Chimie, 55(5), 294-296 (2004).
  27. Y. Wang, H. Xu, J. M. Zhang and G. Li, Sensors, 8, 2043-2081 (2008). https://doi.org/10.3390/s8042043
  28. U. Yogeswaran and S. M. Chen, Sensors, 8, 290-313 (2008). https://doi.org/10.3390/s8010290
  29. J. Chen and Y. J. Fang, Sensors, 7, 448-458 (2007).
  30. L. G. Shaidarova, A. V. Gedmina, I. A. Chelnokova and G. K. Budnikov, J. Anal. Chem., 61, 601-608 (2006). https://doi.org/10.1134/S1061934806060165
  31. R. C. Matos, M. A. Augelli, C. L. Lago and L. Angnes, Anal. Chim. Acta, 404, 151-157 (2000). https://doi.org/10.1016/S0003-2670(99)00674-1
  32. P. Janda, J. Weber, L. Dunsch and A. B. P. Lever, Anal. Chem., 68, 960-965 (1996). https://doi.org/10.1021/ac950323r
  33. W. A. Behrens and R. Madere, Anal. Biochem., 165, 102-107 (1987). https://doi.org/10.1016/0003-2697(87)90206-5
  34. R. Shakya and D. A. Navarre, J. Agric. Food Chem., 54, 5253-5260 (2006). https://doi.org/10.1021/jf0605300
  35. A. J. Melendez-Martinez, I. M. Vicario and F. J. Heredia, Food Chem., 101, 177-184 (2007). https://doi.org/10.1016/j.foodchem.2006.01.023
  36. J. Wang, M. P. Chatrathi, B. M. Tian and R. Polsky, Anal. Chem., 72, 2514-2518 (2000). https://doi.org/10.1021/ac991489l
  37. M. W. Davey, G. Bauw and M. VanMontagu, J. Chromatogr. B, 697, 269-276 (1997). https://doi.org/10.1016/S0378-4347(96)00536-1
  38. M. A. Kall and C. Andersen, J. Chromatogr. B, 730, 101-111 (1999). https://doi.org/10.1016/S0378-4347(99)00193-0
  39. B. Klejdus, J. Petrlova, D. Potesil, V. Adam, R. Mikelova, J. Vacek, R. Kizek and V. Kuban, Anal. Chim. Acta, 520, 57-67 (2004). https://doi.org/10.1016/j.aca.2004.02.027
  40. T. Wu, Y. Q. Guan and J. N. Ye, Food Chem., 100, 1573-1579 (2007). https://doi.org/10.1016/j.foodchem.2005.12.042
  41. E. J. Oliveira and D. G. Watson, J. Chromatogr. B, 764, 3-25 (2001). https://doi.org/10.1016/S0378-4347(01)00401-7
  42. H. Iwase and I. Ono, J. Chromatogr A, 806(2), 361-364 (1998). https://doi.org/10.1016/S0021-9673(98)00062-4