The stability comparison of purified bee venom and bee venom melittin in aqueous solution

수용액에서 정제봉독 (PBV)과 봉독멜리틴 (BVM)의 안정성 비교

  • Received : 2016.05.03
  • Accepted : 2016.08.24
  • Published : 2016.08.25


The stability comparison tests on purified bee venom (PBV) and bee venom melittin (BVM) in different conditions of temperature, solvent, and concentration were studied. High purity BVM (98.2 %) was separated from PBV by prep-HPLC (column, C4) and used to stability tests in aqueous phase. The stability of the PBV has been increased in the saline solution, while BVM was reduced. In distilled water, the stability of PBV has been reduced, while BVM showed an increasing result. As a result, the appropriate conditions for maintaining the long term stability of BVM were found to be the low temperature (4 ℃), distilled water, and concentration (1.0 mg/mL).


Stability;Quantitative analysis;Prep-HPLC;Purified bee venom (PBV);Bee venom melittin (BVM)


  1. S. M. Han, K. G. Lee, J. H. Yeo, H. Y. Kwon, H. J. Baek and K. K. Park, J. Med. Plants. Res., 4(6), 459-464 (2010).
  2. M. H. Kim, Education Plan of Korea, 67-141 (1992).
  3. S. T. Kim, J. Y. Hwang, M. S. Sung, S. Y. Je, D. R. Bae, S. M. Han and S. H. Lee, Korean J. Vet. Serv., 29(1), 19-26 (2006).
  4. H. K. Ko and K. R. Kwon, Publication of KyungHee University, 106-107 (2003).
  5. Z. J. Kokot and J. Matysiak, Chromatographia, 69, 1401-1405 (2009).
  6. R. V. Ameratunga, R. Hawkins, R. Prestidge and J. Marbrook, Pathology, 27(2), 157-160 (1995).
  7. H. R. Chmielewska and T. Szezesna, J. Apic. Sci., 48, 103-109 (2004).
  8. T. C. Terwilliger and D. Eisenberg, J. Biological Chem., 257(11), 6016-6022 (1982).
  9. J. D. Dunn and J. J. Killion, Acta Endocrinol., 119(3), 339-344 (1988).
  10. H. N. Kim and S. S. Nam, Korean Inst. of Herbal Acupunc., 3(1), 65-87 (2000).
  11. J. S. Choi, J. W. Park and M. S, Oh, Daejeon University Orient. Med. Symp., 15(1), 141-160 (2006).
  12. W. Neumann, E. Habermann and G. Amend, Naturwissenschaften, 39, 286-287 (1952).
  13. J. S. Lee, J. Y. Lee, K. R. Kwon and H. C. Lee, J. Pharmacopunct., 9(3), 61-77 (2006).
  14. J. D. Lee, J. Korea Orient. Med., 21(3), 3-8 (2000).
  15. M. Cho, S. Han, J. Kim, J. Yeo, I. Hong, S. Woo and K. Lee, J. Seric. Entomol. Sci., 52(1), 6-9 (2014).
  16. Y. Miura, Eur, Biophys. J., 41, 629-636 (2012).
  17. P. Venkatesu, M. J. Lee and H. M. Lin, Biochem. Eng. J., 32, 157-170 (2006).
  18. C. Liao, M. S. Selvan and J. Zhao, etc., J. Phys. Chem. B., 119(33), 10390-10398 (2015).