- Volume 56 Issue 3
DOI QR Code
Isolation and characterization of Brucella abortus isolates from wildlife species in South Korea
- Truong, Quang Lam (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
- Kim, Kiju (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
- Kim, Jong-Taek (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
- Her, Moon (OIE Reference Laboratory for Brucellosis, Animal and Plant Quarantine Agency) ;
- Jung, Suk-Chan (OIE Reference Laboratory for Brucellosis, Animal and Plant Quarantine Agency) ;
- Hahn, Tae-Wook (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
- Received : 2016.05.07
- Accepted : 2016.06.29
- Published : 2016.09.30
A total of 782 blood and 465 tissue samples from 1,039 wild animals and 127 dairy goats were collected from January 2011 to December 2013 in 10 provinces of South Korea and tested for the presence of brucellosis. The Rose Bengal test revealed that 8.0% (52/650) of the serum samples were seropositive, while 4.2% (33/782) of the serum samples were positive for Brucella antibodies by competitive enzyme-linked immunosorbent assay. Of the 650 sera examined, only 16 (2.5%) were positive by both serological tests. Direct polymerase chain reaction (PCR) assay using B4/B5 primers for Brucella abortus (BCSP31) revealed the prevalence of Brucella to be 26.5% (129/487) in blood samples and 21% (98/465) in tissue samples while, 16S rRNA PCR detected Brucella DNA in 6.8% (33/487) and 2.6% (12/465) in blood and tissue samples, respectively. Of PCR-positive samples, only 6.2% (30/487) of blood samples and 2.4% (11/465) of tissue samples were found to be positive by both BCSP31 and 16S rRNA PCRs. However, Brucella strains were isolated by blood culture from only two out of 487 blood samples (0.4%). This characterization and identification of pathogenic Brucella isolates is the first to clearly indicate that the organisms were Brucella abortus biovar 1.
Supported by : Animal and Plant Quarantine Agency (QIA), Kangwon National University
- Ahmed YF, Sokkar SM, Desouky HM, Ghazi YA, Amin AS, Madboly AA. Pathological and molecular studies on mammary glands and supramammary lymph nodes of naturally Brucella infected buffalo-cows. J Reprod Infertil 2010, 1, 33-40.
- Al-Ajlan HH, Ibrahim ASS, Al-Salamah AA. Comparison of different PCR methods for detection of Brucella spp. in human blood samples. Pol J Microbiol 2011, 60, 27-33.
- Alton GG, Jones LM, Pietz DE. Laboratory techniques in brucellosis. Monogr Ser World Health Organ 1975, 1-163.
- Baddour MM, Alkhalifa DH. Evaluation of three polymerase chain reaction techniques for detection of Brucella DNA in peripheral human blood. Can J Microbiol 2008, 54, 352-357. https://doi.org/10.1139/W08-017
- Baek BK, Lim CW, Rahman MS, Kim CH, Oluoch A, Kakoma I. Brucella abortus infection in indigenous Korean dogs. Can J Vet Res 2003, 67, 312-314.
- Baily GG, Krahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 1992, 95, 271-275.
- Biancifiori F, Garrido F, Nielsen K, Moscati L, Duran M, Gall D. Assessment of a monoclonal antibody-based competitive enzyme linked immunosorbent assay (cELISA) for diagnosis of brucellosis in infected and Rev. 1 vaccinated sheep and goats. New Microbiol 2000, 23, 399-406.
- Da Costa M, Guillou JP, Garin-Bastuji B, Thiebaud M, Dubray G. Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol 1996, 81, 267-275. https://doi.org/10.1111/j.1365-2672.1996.tb04328.x
- Etter RP, Drew ML. Brucellosis in elk of eastern Idaho. J Wildl Dis 2006, 42, 271-278. https://doi.org/10.7589/0090-3558-42.2.271
- Ewalt DR. Comparison of three culture techniques for the isolation of Brucella abortus from bovine supramammary lymph nodes. J Vet Diagn Invest 1989, 1, 227-230. https://doi.org/10.1177/104063878900100306
- Gardner IA, Hietala S, Boyce WM. Validity of using serological tests for diagnosis of diseases in wild animals. Rev Sci Tech 1996, 15, 323-335. https://doi.org/10.20506/rst.15.1.926
- Godfroid J. Brucellosis in wildlife. Rev Sci Tech 2002, 21, 277-286. https://doi.org/10.20506/rst.21.2.1333
- Her M, Cho DH, Kang SI, Lim JS, Kim HJ, Cho YS, Hwang IY, Lee T, Jung SC, Yoo HS. Outbreak of brucellosis in domestic elk in Korea. Zoonoses Public Health 2010, 57, 155-161. https://doi.org/10.1111/j.1863-2378.2009.01287.x
- Hini V, Brodard I, Thomann A, Holub M, Miserez R, Abril C. IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology. BMC Vet Res 2009, 5, 22. https://doi.org/10.1186/1746-6148-5-22
- Kang SI, Her M, Kim JW, Kim JY, Ko KY, Ha YM, Jung SC. Advanced multiplex PCR assay for differentiation of Brucella species. Appl Environ Microbiol 2011, 77, 6726-6728. https://doi.org/10.1128/AEM.00581-11
- Kim JY, Her M, Kang SI, Lee K, Lee HK, Jung SC. Epidemiologic relatedness between Brucella abortus isolates from livestock and wildlife in South Korea. J Wildl Dis 2013, 49, 451-454. https://doi.org/10.7589/2012-07-188
- Leal-Klevezas DS, Martinez-Vazquez IO, Lopez-Merino A, Martinez-Soriano JP. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals. J Clin Microbiol 1995, 33, 3087-3090.
- Lopez-Goni I, Garcia-Yoldi D, Marin CM, de Miguel MJ, Munoz PM, Blasco JM, Jacques I, Grayon M, Cloeckaert A, Ferreira AC, Cardoso R, Correa de Sa MI, Walravens K, Albert D, Garin-Bastuji B. Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. J Clin Microbiol 2008, 46, 3484-3487. https://doi.org/10.1128/JCM.00837-08
- Mainar-Jaime RC, Munoz PM, de Miguel MJ, Grillo MJ, Marin CM, Moriyon I, Blasco JM. Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9. Can Vet J 2005, 46, 913-916.
- Mathias LA, Girio RJS, Duarte JMB. Serosurvey for antibodies against Brucella abortus and Leptospira interrogans in pampas deer from Brazil. J Wildl Dis 1999, 35, 112-114. https://doi.org/10.7589/0090-3558-35.1.112
- Mukherjee F, Jain J, Patel V, Nair M. Multiple genusspecific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals. J Med Microbiol 2007, 56, 1309-1316. https://doi.org/10.1099/jmm.0.47160-0
- Munoz PM, Marin CM, Monreal D, Gonzalez D, Garin- Bastuji B, Diaz R, Mainar-Jaime RC, Moriyon I, Blasco JM. Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9. Clin Diagn Lab Immunol 2005, 12, 141-151.
- Navarro E, Escribano J, Fernandez J, Solera J. Comparison of three different PCR methods for detection of Brucella spp. in human blood samples. FEMS Immunol Med Microbiol 2002, 34, 147-151. https://doi.org/10.1111/j.1574-695X.2002.tb00616.x
- Navarro E, Fernandez JA, Escribano J, Solera J. PCR assay for diagnosis of human brucellosis. J Clin Microbiol 1999, 37, 1654-1655.
- OIE. Bovine brucellosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chap. 2.4.3. pp. 624-659, World Orgarnisation for Animal Health, Paris, 2009.
- Perrett LL, McGiven JA, Brew SD, Stack JA. Evaluation of competitive ELISA for detection of antibodies to Brucella infection in domestic animals. Croat Med J 2010, 51, 314-319. https://doi.org/10.3325/cmj.2010.51.314
- Qureshi T, Stittmatter J, Turner K, Davis DS. Experimental infection of white-tailed deer with rangiferine brucellosis. J Wildl Dis 1999, 35, 388-391. https://doi.org/10.7589/0090-3558-35.2.388
- Romero C, Gamazo C, Pardo M, Lopez-Goni I. Specific detection of Brucella DNA by PCR. J Clin Microbiol 1995, 33, 615-617.
- Samartino L, Gall D, Gregoret R, Nielsen K. Validation of enzyme-linked immunosorbent assays for the diagnosis of bovine brucellosis. Vet Microbiol 1999, 70, 193-200. https://doi.org/10.1016/S0378-1135(99)00122-4
- Tiller RV, Gee JE, Frace MA, Taylor TK, Setubal JC, Hoffmaster AR, De BK. Characterization of novel Brucella strains originating from wild native rodent species in North Queensland, Australia. Appl Environ Microbiol 2010, 76, 5837-5845. https://doi.org/10.1128/AEM.00620-10
- Truong QL, Kim JT, Yoon BI, Her M, Jung SC, Hahn TW. Epidemiological survey for Brucella in wildlife and stray dogs, a cat and rodents captured on farms. J Vet Med Sci 2011, 73, 1597-1601. https://doi.org/10.1292/jvms.11-0222
- Wee SH, Nam HM, Kim CH. Emergence of brucellosis in cattle in the Republic of Korea. Vet Rec 2008, 162, 556-557. https://doi.org/10.1136/vr.162.17.556
- Weiner M, Iwaniak W, Szulowski K. Identification of Brucella DNA in lymph tissue from deer (Cervus elaphus) and wild boars (Sus scrofa) by the use of BCSP31 PCR and AMOSPCR. Bull Vet Inst Pulawy 2009, 53, 609-612.
- Yoon H, Moon OK, Lee SH, Lee WC, Her M, Jeong W, Jung SC, Kim DS. Epidemiology of brucellosis among cattle in Korea from 2001 to 2011. J Vet Sci 2014, 15, 537-543. https://doi.org/10.4142/jvs.2014.15.4.537
- Yu WL, Nielsen K. Review of detection of Brucella spp. by polymerase chain reaction. Croat Med J 2010, 51, 306-313. https://doi.org/10.3325/cmj.2010.51.306
- Zheludkov MM, Tsirelson LE. Reservoirs of Brucella infection in nature. Biol Bull 2010, 37, 709-715. https://doi.org/10.1134/S106235901007006X