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Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • Park, Mi-Ja (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Joh, Seong-Joon (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Choi, Kang-Seuk (Avian Diseases Division, Animal and Plant Quarantine Agency) ;
  • Kim, Aeran (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Seo, Min-Goo (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Song, Jae-Young (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency) ;
  • Yun, Seon-Jong (Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency)
  • Received : 2016.04.18
  • Accepted : 2016.07.01
  • Published : 2016.09.30

Abstract

The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

Acknowledgement

Supported by : Animal and Plant Quarantine Agency

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