Bee Venom (Apis Mellifera) an Effective Potential Alternative to Gentamicin for Specific Bacteria Strains - Bee Venom an Effective Potential for Bacteria-

  • Zolfagharian, Hossein (Department of Venomous Animals and Antivenom Production, Razi Vaccine and Serum Research Institute) ;
  • Mohajeri, Mohammad (Department of Medical Biotechnology, School of Medicine, Mashhad University of medical Science) ;
  • Babaie, Mahdi (Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University)
  • Received : 2016.03.23
  • Accepted : 2016.07.31
  • Published : 2016.09.30


Objectives: Mellitine, a major component of bee venom (BV, Apis mellifera), is more active against gram positive than gram negative bacteria. Moreover, BV has been reported to have multiple effects, including antibacterial, antivirus, and anti-inflammation effects, in various types of cells. In addition, wasp venom has been reported to have antibacterial properties. The aim of this study was to evaluate the antibacterial activity of BV against selected gram positive and gram negative bacterial strains of medical importance. Methods: This investigation was set up to evaluate the antibacterial activity of BV against six grams positive and gram negative bacteria, including Staphylococcus aureus (S. aureus), Salmonella typhimurium, Escherichia coli (E. coli) O157:H7, Pseudomonas aeruginosa, Burkholderia mallei and Burkholderia pseudomallei. Three concentrations of crude BV and standard antibiotic (gentamicin) disks as positive controls were tested by using the disc diffusion method. Results: BV was found to have a significant antibacterial effect against E. coli, S. aureus, and Salmonella typhyimurium in all three concentrations tested. However, BV had no noticeable effect on other tested bacteria for any of the three doses tested. Conclusion: The results of the current study indicate that BV inhibits the growth and survival of bacterial strains and that BV can be used as a complementary antimicrobial agent against pathogenic bacteria. BV lacked the effective proteins necessary for it to exhibit antibacterial activity for some specific strains while being very effective against other specific strains. Thus, one may conclude, that Apis mellifera venom may have a specific mechanism that allows it to have an antibacterial effect on certain susceptible bacteria, but that mechanism is not well understood.


  1. Hider RC. Honeybee venom: a rich source of pharmacologically active peptides. Endeavour. 1988;12(2):60-5.
  2. Hegazi A, Abdou AM, Abd El-Moez SI, Abd F. Evaluation of the antibacterial activity of bee venom from different sources. World Appl Sci J. 2014;30(3):266-70.
  3. Jenssen H, Hamill P, Hancock RE. Peptide antimicrobial agents. Clin Microbiol Rev. 2006;19(3):491-511.
  4. Wenhua R, Shuangquan Z, Daxiang S, Kaiya Z, Guang Y. Induction, purification and characterization of an antibacterial peptide scolopendrin I from the venom of centipede Scolopendra subspinipes multilans. Indian J Biochem Biophys. 2006;43:88-93.
  5. Castro HJ, Mendez-Lnocencio JI, Omidvar B, Omidvar J, Santilli J, Nielsen HS Jr, et al. A phase I study of the safety of honeybee venom extract as a possible treatment for patients with progressive forms of multiple sclerosis. Allergy Asthma Proc. 2005;26(6):470-6.
  6. Fennell JF, Shipman WH, Cole LJ. Antibacterial action of bee venom fraction against a penicillin-resistant Staphylococcus and other microorganisms. Res Dev Tech Rep. 1967;5:1-13.
  7. Fennell JF, Shipman WH, Cole LJ. Antibacterial action of Melittin, polypeptide from bee venom. Proc Soc Exp Biol Med. 1968;127(3):707-10.
  8. Dani MP, Richards EH, Isaac RE, Edwards JP. Antibacterial proteolytic activity in venom from the endoparasitic wasp Pimple hypochondriaca (hymenoptera: Ichneumonidae). J Insect Physiol. 2003;49(10):945-54.
  9. Permual SR, Pachiappan A, Gopalakrishnakone P, Thwin MM, Hian YE, Chow VT, et al. In vitro antibacterial activity of natural toxins and animal venoms tested against Burkholderia Pseudomallei. BMC Infect Dis. 2006;6:100.
  10. Hegazi AG, EL-Feel MA, Abdel-Rahman EA, Al-Fattah A. Antibacterial activity of bee venom collected from apis mellifera carniolan pure and hybrid races by two collection methods. Int J Curr Microbiol App Sci. 2015;4(4):141-9.
  11. Bauer AW, Kirdy WM, Sherries JC, Turck M. Antibiotic susceptibility testing by standardized signal disk method. Am J Clin Pathol. 1966;45(4):493-6.
  12. Surendra NS, Jayaram GN, Reddy MS. Antimicrobial activity of crude venom extracts in honeybees (Apis cerana, Apis dorsata, Apis florea) tested against selected pathogens. Afr J Microbiol Res. 2011;5(18):2765-72.
  13. Park JW, Jeon JH, Yoon J, Jung TY, Kwon KR, Cho CK, et al. Effects of sweet bee venom pharmacopuncture treatment for chemotherapy-induced peripheral neuropathy: a case series. Integr Cancer Ther. 2012;11(2):166-71.
  14. Kim HW, Kwon YB, Ham TB, Roh DH, Yoon SY, Lee HJ. Acupoint stimulation using bee venom attenuates formalin-induced pain behavior and spinal cord fos expression. J Vet Med Sci. 2003;65(3):349-55.
  15. Kim KS, Choi US, Lee SD, Kim KH, Chung KH, Chang YC, et al. Effect of bee venom on aromatase expression and activity in leukaemic FLG 29.1 and primary osteoblastic cells. J Ethnopharmacol. 2005;99(2):245-52.
  16. Leandro LF, Mendes CA, Casemiro LA, Vinholis AH, Cunha WR, de Almeida R, et al. Antimicrobial activity of apitoxin, melittin and phospholipase$A_2$ of honey bee (Apis mellifera) venom against oral pathogens. An Acad Bras Cienc. 2015;87(1):147-55.
  17. Cujova S, Bednarova L, Slaninova J, Straka J, Cerovsky V. Interaction of a novel antimicrobial peptide isolated from the venom of solitary bee colletes daviesanus with phospholipid vesicles and Escherichia coli cells. J Pept Sci. 2014;20(11):885-95.
  18. Ortel S, Markwardt F. Studies on the antibacterial properties of bee venom. Pharmazie. 1955;10:743-6.
  19. Lariviere WR, Melzack R. The bee venom test: a new tonic-pain test. Pain. 1996;66(2-3):271-7.
  20. Nunez V, Arce V, Gutierrez JM, Lomonte B. Structuer and functional characterization of myotoxin I, a LYS49 phospholipase A2 homologus from the snake bothrops atrox. Toxicon. 2004;44(1):91-101.
  21. Permual SR, Gopalakrishnakone P, Thwin MM, Chow TK, Bow H, Yap EH, et al. Antibacterial activity of snake, scorpion and bee venoms: a comparison with purified venom phospholipase A2 enzymes. J Appl Microbiol. 2007;102(3):650-9.
  22. Monk JD, Beuchat LR, Hathcox AK. Inhibitory effects of sucrose monolaurate, alone and in combination with organic acids, on Listeria monocytogenes and Staphylococcusaureus. J Appl Bacteriol. 1996;81(1):7-18.
  23. Kondo E, Kanai K. Bactericidal activity of the membrane fraction isolated form phagocytes of mice and its stimulation by melittin. Japan J Med Sci & Biol. 1986;39:9-20.
  24. Hegazi AG, Moharm NZ, Allah FA, Nour MS, Khair AM. Antibacterial activity of different Egyptian honeys in relation to some bee products. Egypt J Vet Sci. 2002;36:31-42.
  25. Rybak CH, Szczesna T, Rybak M, Pidek A. Some properties of honey bee venom. Pszczelnicze Zeszyty Naukowe. 1994;38:85-90.