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Development of Analytical Reference Material for Proficiency Test of Pesticide Multi-residue Analysis in Green-pepper

풋고추 농약다성분분석 정도관리용 분석표준물질 개발

  • Kim, Jong-Hwan (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Choi, Sung-Gil (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Oh, Young-Gon (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Kwon, Young-Sang (Environmental Toxicology Research Center, Korea Institute of Toxicology) ;
  • Hong, Su-Myeong (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Sung, Mun-Hyun (Namyangju-si Agricultural Technology Center) ;
  • Lee, Se-Ja (Wanju-Gun Agricultural Technology Center) ;
  • Hwang, Sun-Young (Wanju-Gun Agricultural Technology Center) ;
  • Seo, Jong-Su (Environmental Toxicology Research Center, Korea Institute of Toxicology)
  • 김종환 (안전성평가연구소 환경독성연구센터) ;
  • 최성길 (안전성평가연구소 환경독성연구센터) ;
  • 오영곤 (안전성평가연구소 환경독성연구센터) ;
  • 권영상 (안전성평가연구소 환경독성연구센터) ;
  • 홍수명 (국립농업과학원 농산물안전성부 화학물질안전과) ;
  • 성문현 (남양주시농업기술센터) ;
  • 이세자 (완주군농업기술센터) ;
  • 황선영 (완주군농업기술센터) ;
  • 서종수 (안전성평가연구소 환경독성연구센터)
  • Received : 2016.07.20
  • Accepted : 2016.09.11
  • Published : 2016.09.30

Abstract

This study was to develop the analytical reference material of green-pepper for multi-residue analysis of pesticides. According to the ISO Guide 35, ISO Guide 13528 and EURL-PT protocol, the homogeneity, stability, assigned value and uncertainty were calculated to assess if it was suitable to be used as the proficiency test or quality control. The values of the within-bottle standard variation ($s_{wb}$) and the between-bottle standard variation ($s_{bb}$) were 1.7~3.7% of assigned value according to the requirement of the ISO guide 35. And, the uncertainty ($u^*{_{bb}}$) due to inhomogeneity was 0.8~1.1% for all pesticides. The storage stabilities of ten-pesticides at various conditions were assessed. For all target pesticides, the slop ($b_1$) values were smaller than the corresponding values of $[t_{0.95,n-2}{\times}s(b_1)]$ specified by the ISO guide 35, indicating that there were no statistically significant decreases in the concentration of the target pesticides when the analytical reference material was stored at room temperature ($20{\sim}30^{\circ}C$) for 7 days, freezing ($-20^{\circ}C$) for 30 days and deep freezer ($-80^{\circ}C$. except for bifenthrin, fenpropathrin) for 245 days. For proficiency test by using it developed by Korea Institute of Toxicology, inter-lab test was performed with eight organization performing the residual pesticide analysis. We found that there were some different results among them. Some were assessed as questionable or unacceptable for two pesticides and one organization didn't analyze the six pesticides. From these results, this green-pepper analytical reference material containing ten-pesticides could be used as a tool for the proficiency test to improve the reliability or consistency for pesticide residue's results.

Acknowledgement

Supported by : 농촌진흥청

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