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Determination of Pyribencarb and Its Metabolite KIE-9749 in Agricultural Commodities by High-Performance Liquid Chromatography

액체크로마토그래피를 이용한 농산물중 pyribencarb와 대사물 KIE-9749의 잔류 분석법

  • Kwon, Hyeyoung (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hong, Su-Myeong (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Taek-Kyum (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Hyung-Jin (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Moon, Byeong-Chul (Chemical Safety Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kyung, Kee-Sung (Department of Environmental and Biological Chemistry, College of Agricultural Life and Environmental Sciences, Chungbuk National University)
  • 권혜영 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 홍수명 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 김택겸 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 김형진 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 문병철 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 경기성 (충북대학교 환경생명화학과)
  • Received : 2016.04.18
  • Accepted : 2016.09.28
  • Published : 2016.09.30

Abstract

The aim of this study was to develop an analytical method for the determination of pyribencarb and its metabolite KIE-9749 in agricultural commodities. The experiment was performed with a range of concentrations $0.05{\sim}2.5{\mu}g/g$ in apple, green pepper, potato, hulled rice, soybean, pear, peach, grape and cucumber. Each samples were extracted with acetone and cleaned by dichloromethane/saline water partition and purified with Florisil solid phase extraction (SPE) cartridge and aminopropyl SPE cartridge. Pyribencarb and KIE-9749 were separated and quantified by HPLC/UVD at 265nm using acetonitrile and water as mobile phase. The recoveries of pyribencarb and KIE-9749 were within 78.3~108.4% and 73.9~113.7% with RSD below 12.2% and 15.0%, respectively. The limits of quantification (LOQ) were both $0.05{\mu}g/g$. LC/ESI-MS/MS was optimized for confirmation of residue identity.

Acknowledgement

Supported by : 국립농업과학원

References

  1. Abreu, S. M., P. Caboni, P. Cabras, V. L. Garau and A. Alves (2006) Validation and global uncertainty of a liquid chromatographic with diode array detection method for the screening of azoxystrobin, kresoxim-methyl, trifloxystrobin, famoxadone, pyraclostrobin and fenamidone in grapes and wine, Analytica Chimica Acta 573:291-297.
  2. Anastassiades, M., S. Lehotay, D. Štajnbaher and F. Schenck (2003) Fast and Easy Multiresidue Method Employing Acetonitrile Extraction/Partitioning and "Dispersive Solid-Phase Extraction" for the Determination of Pesticide Residues in Produce, AOAC International 86(2):412-431.
  3. CAC/GL 40-1993 Revised Guidelines on Good Laboratory Practice in Residue Analysis, Rev.1-2003, http://www.codexalimentarius.net/download/standards/378/cxg_040e.pdf
  4. Chonan, T. (2001) Determination of azoxystrobin in tea by HPLC, Shokuhin Eiseigaku Zasshi 42(4):249-251. https://doi.org/10.3358/shokueishi.42.249
  5. Grimalt, S. and P. Dehouck (2016) Review of analytical methods for the determination of pesticide residues in grapes, Journal of Chromatography A 1433:1-23. https://doi.org/10.1016/j.chroma.2015.12.076
  6. Kataoka, S., M. Takagaki, K. Kaku and T. Shimizu (2010) Mechanism of action and selectivity of a novel fungicide, pyribencarb, Journal of Pesticide Science 35(2):99-106. https://doi.org/10.1584/jpestics.G09-40
  7. Korea Crop Protection Association (2015) Agrochemicals Use Guide Book, pp. 86, 197, 390, Korea Crop Protection Association. Samjeong Publisher, Seoul, Korea.
  8. Li, J. Z., X. Wu and J. Y. Hu (2006) Determination of fungicide kresoxim-methyl residues in cucumber and soil by capillary gas chromatography with nitrogen-phosphorus detection, Journal of Environmental Science and Health Part B 41(4):427-436. https://doi.org/10.1080/03601230600616841
  9. Ministry of Food and Drug Safety (2016) Korea Food Code, Republic of Korea.
  10. Ministry of Food and Drug Safety (2013) Pesticide Analytical Residues Manual in Food Code (4th ed), National Institute of Food and Drug Safety Evaluation, Republic of Korea.
  11. Rural development administration (2011) Food composition table (8th revision), National Institute of Agriculutral Sciences, Republic of Korea.
  12. Rural development administration (2014) Toxicity assessment and safety standard for pyribencarb, National Institute of Agriculutral Sciences, Republic of Korea.
  13. Rural development administration (2015) Residue definition of pesticides for agricultural products, National Institute of Agriculutral Sciences, Republic of Korea.
  14. SANTE/11945/2015 Guidance document on analytical quality control and method validation procedures for pesticides residues analysis in food and feed, http://ec.europa.eu/food/plant/docs/plant_pesticides_mrl_guidelines_wrkdoc_11945_en.pdf
  15. Takagaki, M., M. Ozaki, S. Fujimoto and S. Fukumoto (2014) Development of a novel fungicide, pyribencarb, Journal of Pesticide Science 39(3):177-178. https://doi.org/10.1584/jpestics.J14-04
  16. Tomlin, C.D.S. (2012) The pesticide manual, 16th ed, British Crop Protection Council, UK.
  17. Tuzimski, T. and J. Sherma (2015) High Performance Liquid Chromatography in Pesticide Residue Analysis, CRC Press, USA.
  18. Rural development administration Government 3.0 open information board http://rda.go.kr/board/board.do?prgId=opp_openinfoboard Accessed at 15 April 2016.