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Validation of Single Nucleotide Polymorphisms Associated with Carcass Traits in a Commercial Hanwoo Population

  • Sudrajad, Pita (Division of Animal and Dairy Science, Chungnam National University) ;
  • Sharma, Aditi (Animal Genomics and Bioinformatics Division, National Institute of Animal Science, RDA) ;
  • Dang, Chang Gwon (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Kim, Jong Joo (School of Biotechnology, Yeungnam University) ;
  • Kim, Kwan Suk (Department of Animal Science, Chungbuk National University) ;
  • Lee, Jun Heon (Division of Animal and Dairy Science, Chungnam National University) ;
  • Kim, Sidong (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Lee, Seung Hwan (Division of Animal and Dairy Science, Chungnam National University)
  • Received : 2015.10.12
  • Accepted : 2016.02.23
  • Published : 2016.11.01

Abstract

Four carcass traits, namely carcass weight (CW), eye muscle area (EMA), back fat thickness (BF), and marbling score (MS), are the main price decision parameters used for purchasing Hanwoo beef. The development of DNA markers for these carcass traits for use in a beef management system could result in substantial profit for beef producers in Korea. The objective of this study was to validate the association of highly significant single nucleotide polymorphisms (SNPs) identified in a previous genome-wide association study (GWAS) with the four carcass traits in a commercial Hanwoo population. We genotyped 83 SNPs distributed across all 29 autosomes in 867 steers from a Korean Hanwoo feedlot. Six SNPs, namely ARS-BFGL-NGS-22774 (Chr4, Pos:4889229), ARS-BFGL-NGS-100046 (Chr6, Pos:61917424), ARS-BFGL-NGS-39006 (Chr27, Pos:38059196), ARS-BFGL-NGS-18790 (Chr10, Pos:26489109), ARS-BFGL-NGS-43879 (Chr9, Pos:39964297), and BTB-00775794 (Chr20, Pos:20476265), were found to be associated with CW, EMA, BF, and MS. The ARS-BFGL-NGS-22774, BTB-00775794, and ARS-BFGL-NGS-39006 markers accounted for 1.80%, 1.72%, and 1.35% (p<0.01), respectively, of the phenotypic variance in the commercial Hanwoo population. Many genes located in close proximity to the significant SNPs identified in this study were previously reported to have roles in carcass traits. The results of this study could be useful for marker-assisted selection programs.

Keywords

Validation;Single Nucleotide Polymorphism;Carcass Traits;Hanwoo

Acknowledgement

Supported by : National Institute of Animal Science

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