Effect of Simulated Heat Stress on Digestibility, Methane Emission and Metabolic Adaptability in Crossbred Cattle

  • Yadav, Brijesh (Division of Physiology and Climatology, Indian Veterinary Research Institute) ;
  • Singh, Gyanendra (Division of Physiology and Climatology, Indian Veterinary Research Institute) ;
  • Wankar, Alok (Division of Physiology and Climatology, Indian Veterinary Research Institute) ;
  • Dutta, N. (Division of Animal Nutrition, Indian Veterinary Research Institute) ;
  • Chaturvedi, V.B. (Division of Animal Nutrition, Indian Veterinary Research Institute) ;
  • Verma, Med Ram (Division of Statistics, Indian Veterinary Research Institute)
  • 투고 : 2015.08.24
  • 심사 : 2016.01.05
  • 발행 : 2016.11.01


The present experiment was conducted to evaluate the effect of simulated heat stress on digestibility and methane ($CH_4$) emission. Four non-lactating crossbred cattle were exposed to $25^{\circ}C$, $30^{\circ}C$, $35^{\circ}C$, and $40^{\circ}C$ temperature with a relative humidity of 40% to 50% in a climatic chamber from 10:00 hours to 15:00 hours every day for 27 days. The physiological responses were recorded at 15:00 hours every day. The blood samples were collected at 15:00 hours on 1st, 6th, 11th, 16th, and 21st days and serum was collected for biochemical analysis. After 21 days, fecal and feed samples were collected continuously for six days for the estimation of digestibility. In the last 48 hours gas samples were collected continuously to estimate $CH_4$ emission. Heat stress in experimental animals at $35^{\circ}C$ and $40^{\circ}C$ was evident from an alteration (p<0.05) in rectal temperature, respiratory rate, pulse rate, water intake and serum thyroxin levels. The serum lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activity and protein, urea, creatinine and triglyceride concentration changed (p<0.05), and body weight of the animals decreased (p<0.05) after temperature exposure at $40^{\circ}C$. The dry matter intake (DMI) was lower (p<0.05) at $40^{\circ}C$ exposure. The dry matter and neutral detergent fibre digestibilities were higher (p<0.05) at $35^{\circ}C$ compared to $25^{\circ}C$ and $30^{\circ}C$ exposure whereas, organic matter (OM) and acid detergent fibre digestibilities were higher (p<0.05) at $35^{\circ}C$ than $40^{\circ}C$ thermal exposure. The $CH_4$ emission/kg DMI and organic matter intake (OMI) declined (p<0.05) with increase in exposure temperature and reached its lowest levels at $40^{\circ}C$. It can be concluded from the present study that the digestibility and $CH_4$ emission were affected by intensity of heat stress. Further studies are necessary with respect to ruminal microbial changes to justify the variation in the digestibility and $CH_4$ emission during differential heat stress.


  1. AOAC (Association of Official Analytical Chemist). 1995. Official Methods of Analysis. 16th edn. Association of Official Analytical Chemists, Arlington, VA, USA.
  2. Banerjee, D. and Ashutosh. 2011. Effect of thermal exposure on diurnal rhythms of physiological parameters and feed, water intake in Tharparkar and Karan Fries heifers. Biol. Rhythm Res. 42:39-51.
  3. Barnett, M. C., J. P. Goopy, J. R. McFarlane, I. R. Godwin, J. V. Nolan, and R. S. Hegarty. 2012. Triiodothyronine influences digesta kinetics and methane yield in sheep. Anim. Prod. Sci. 52:572-577.
  4. Barnett, M. C., J. R. McFarlane, and R. S. Hegarty. 2015. Low ambient temperature elevates plasma triiodothyronine concentrations while reducing digesta mean retention time and methane yield in sheep. J. Anim. Physiol. Anim. Nutr. 99:483-491.
  5. Beatty, D. T., A. Barnes, E. Taylor, and S. K. Maloney. 2008. Do changes in feed intake or ambient temperature cause changes in cattle rumen temperature relative to core temperature? J. Therm. Biol. 33:12-19.
  6. Benchaar, C., C. Pomar, and J. Chiquette. 2001. Evaluation of dietary strategies to reduce methane production in ruminants: A modeling approach. Can. J. Anim. Sci. 81:563-574.
  7. Bernabucci, U., P. Bani, B. Ronchi, N. Lacetera, and A. Nardone. 1999. Influence of short- and long-term exposure to hot environment on rumen passage rate and diet digestibility by Friesian heifers. J. Dairy Sci. 82:967-973.
  8. Bernabucci, U., N. Lacetera, P. P. Danieli, P. Bani, A. Nardone, and B. Ronchi. 2009. Influence of different periods of exposure to hot environment on rumen function and diet digestibility in sheep. Int. J. Biometeorol. 53:387-395.
  9. Gorniak, T., U. Meyer, K. H. Sudekum, and S. Danicke. 2014. Effect of ambient temperature on nutrient digestibility and nitrogen balance in sheep fed brown-midrib maize silage. Arch. Anim. Nutr. 68:336-344.
  10. IPCC (Intergovernmental Panel on Climate Change). 2007. The Intergovernmental Panel on Climate Change. 4th Assessment Report, IPCC, Geneva, Switzerland.
  11. Kittelmann, S., C. S. Pinares-Patino, H. Seedorf, M. R. Kirk, S. Ganesh, J. C. McEwan, and P. H. Janssen. 2014. Two different bacterial community types are linked with the low-methane emission trait in sheep. PLoS One9:e103171.
  12. Lourenco, A. L., J. W. Cone, P. Fontes, and A. A. Dias-da-Silva. 2010. Effects of ambient temperature and soybean meal supplementation on intake and digestion of two sheep breeds differing in mature size. J. Anim. Physiol. Anim. Nutr. 94:571-583.
  13. Marai, I. F. M., A. A. El-Darawany, E. I. Abou-Fandoud, and M. A. M. Abdel-Hafez. 2009. Reproductive and physiological traits of Egyptian Suffolk rams as affected by selenium dietary supplementation during the sub-tropical environment of Egypt.Livest. Res. Rural Dev. 21:Article #157.
  14. McDowell, R. E., E. G. Moody, P. J. Van Soest, and R. P. Lehmann. 1969. Effect of heat stress on energy and water utilization of lactating cows. J. Dairy Sci. 52:188-194.
  15. McManus, C., G. R. Paludo, H. Louvandini, R. Gugel, L. C. B. Sasaki, and S. R. Paiva. 2009. Heat tolerance in Brazilian sheep: physiological and blood parameters. Trop. Anim. Health Prod. 41:95-101.
  16. Monteny, G. J., C. M. Groenestein, and M. A. Hilhorst. 2001. Interactions and coupling between emissions of methane and nitrous oxide from animal husbandry. Nutrient Cycling in Agroecosystems 60:123-132.
  17. NRC (National Research Council). 1989. Nutrient Requirements of Dairy Cattle. 6th revised edn. National Academy Press, Washington, DC, USA.
  18. Ngwabie, N. M., K. H. Jeppsson, G. Gustafsson, and S. Nimmermark. 2011. Effects of animal activity and air temperature onmethane and ammonia emissions from a naturally ventilated building for dairy cows. Atmos. Environ. 45:6760-6768.
  19. Nkrumah, J. D., E. K. Okine, G. D. Mathison, K. Schmid, C. Li, J. A. Basarab, M. A. Price, Z. Wang, and S. S. Moore. 2006. Relationship of feedlot feed efficiency, performance, and feeding behavior with metabolic rate, methane production, and energy partitioning in beef cattle. J. Anim. Sci. 84:145-153.
  20. Nonaka, I., N. Takusari, K. Tajima, T. Suzuki, K. Higuchi, and M. Kurihara. 2008. Effects of high environmental temperatures on physiological and nutritional status of prepubertal Holstein heifers. Livest. Sci. 113:14-23.
  21. Ravagnolo, O., I. Misztal, and G. Hoogenboom. 2000. Genetic component of heat stress in dairy cattle, development of heat index function. J. Dairy Sci. 83:2120-2125.
  22. Ronchi, B., U. Bernabucci, N. Lacetera, A. Verini Supplizi, and A. Nardone. 1999. Distinct and common effects of heat stress and restricted feeding on metabolic status in Holstein heifers. Zoot. Nutr. Anim. 25:11-20.
  23. SAS. 2014. Base SAS 9.4 Procedures Guide: Statistical Procedures. SAS Institute Inc., Cary, NC, USA.
  24. Seguin, B. 2008. The consequences of global warming for agriculture and food production. In: Proceedings of the Livestock and Global Climate Change (Eds. P. Rowlinson, M. Steele, and A. Nefzaoui). Cambridge University Press, Hammamet, Tunisia. pp. 9-11.
  25. Sejian, V., A. K. Singh, A. Sahoo, and S. M. K. Naqvi. 2014. Effect of mineral mixture and antioxidant supplementation on growth, reproductive performance and adaptive capability of Malpura ewes subjected to heat stress. J. Anim. Physiol. Anim. Nutr. (Berl) 98:72-83.
  26. Srikandakumar, A., E. H. Johnson, and O. Mahgoub. 2003. Effect of heat stress on respiratory rate, rectal temperature and blood chemistry in Omani and Australian Merino sheep. Small Rumin. Res. 49:193-198.
  27. Thornton, P. K. 2010. Livestock production: Recent trends, future prospects. Philos. Trans. R. Soc. Lond., B., Biol. Sci. 365:2853-2867.
  28. Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods of dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3587.
  29. West, J. W. 2003. Effects of heat-stress on production in dairy cattle. J. Dairy Sci. 86:2131-2144.
  30. Wheelock, J. B., R. P. Rhoads, M. J. VanBaale, S. R. Sanders, and L. H. Baumgard. 2010. Effects of heat stress on energetic metabolism in lactating Holstein cows. J. Dairy Sci. 93:644-655.
  31. Yadav, B., G. Singh, and A. Wankar. 2015. Adaptive capability as indicated by redox status and endocrine responses in crossbred cattle exposed to thermal stress. J. Anim. Res. 5:67-73.
  32. Yadav, B., G. Singh, A. K. Verma, N. Dutta, and V. Sejian. 2013. Impact of heat stress on rumen functions. Vet. World 6:992-996.

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