Use of Lysozyme as a Feed Additive on In vitro Rumen Fermentation and Methane Emission

  • Biswas, Ashraf A. (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University) ;
  • Lee, Sung Sill (Division of Applied Life Science (BK21+, IALS), Gyeongsang National University) ;
  • Mamuad, Lovelia L. (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University) ;
  • Kim, Seon-Ho (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University) ;
  • Choi, Yeon-Jae (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University) ;
  • Bae, Gui-Seck (Department of Animal Science and Technology, Chung-Ang University) ;
  • Lee, Kichoon (Department of Animal Sciences, The Ohio State University) ;
  • Sung, Ha-Guyn (Department of Animal Science & Technology, Sangji University) ;
  • Lee, Sang-Suk (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University)
  • Received : 2016.07.28
  • Accepted : 2016.09.28
  • Published : 2016.11.01


This study was conducted to determine the effect of lysozyme addition on in vitro rumen fermentation and to identify the lysozyme inclusion rate for abating methane ($CH_4$) production. An in vitro ruminal fermentation technique was done using a commercial concentrate to rice straw ratio of 8:2 as substrate. The following treatments were applied wherein lysozyme was added into 1 mg dry matter substrate at different levels of inclusion: Without lysozyme, 2,000, 4,000, and 8,000 U lysozyme. Results revealed that, lysozyme addition had a significant effect on pH after 24 h of incubation, with the highest pH (p<0.01) observed in 8,000 U lysozyme, followed by the 4,000 U, 2,000 U, and without lysozyme. The highest amounts of acetic acid, propionic acid (p<0.01) and total volatile fatty acid (TVFA) (p<0.05) were found in 8,000 U after 24 h of incubation. The $CH_4$ concentration was the lowest in the 8,000 U and the highest in the without lysozyme addition after 24 h of incubation. There was no significant differences in general bacteria, methanogen, or protozoan DNA copy number. So far, addition of lysozyme increased the acetate, propionate, TVFA, and decreased $CH_4$ concentration. These results suggest that lysozyme supplementation may improve in vitro rumen fermentation and reduce $CH_4$ emission.


In vitro;Lysozyme;Methane;Ruminant;Volatile Fatty Acid


Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries


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